2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) induces wide metabolic changes including attenuated mitochondrial function and enhanced glycolysis in PC12 cells

He, Hailang; Shi, Xiaojian; Lawrence, Alex; Hrovat, Jonathan; Turner, C. H.; Cui, Julia Yue; Gu, Haiwei

doi:10.1016/j.ecoenv.2020.110849

Cited by 31 publications

(19 citation statements)

References 81 publications

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“…Secondly, in metabolomics studies, the differences in viability between the control and treated cells would affect the accuracy of the results. The dose of IC50 has been used in the metabolomics research of toxicology in recent years ( Yu et al, 2019 ; He et al, 2020 ; Hou et al, 2020 ). To capture more responses on cell metabolism related to the anticancer effect of EGCG and explore the changes in metabolic processes with increasing EGCG concentration, the dosages of 40 and 80 μM were both used in our metabolomics experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolomics is an exciting tool to detect small metabolic compounds and monitor small global molecule endogenous metabolite changes induced by biochemical reactions in biological systems (Dunn et al, 2011;Griffin et al, 2011;Reaves and Rabinowitz, 2011;Gu et al, 2012;Carroll et al, 2015;Zampieri et al, 2017;McCartney et al, 2018;Yan and Xu, 2018;Yeung, 2018;Shi et al, 2019;Eghlimi et al, 2020;He et al, 2020;Lim et al, 2020;Wei et al, 2021). Metabolomics is a promising approach to searching potential biomarkers and novel therapeutic strategies for lung cancer (Luengo et al, 2017;Seijo et al, 2019;Noreldeen et al, 2020;Schmidt et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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LC–MS Based Metabolomics Study of the Effects of EGCG on A549 Cells

Pan

Han

et al. 2021

Front. Pharmacol.

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(−)-Epigallocatechin-3-gallate (EGCG) is the main bioactive catechin in green tea. The antitumor activity of EGCG has been confirmed in various types of cancer, including lung cancer. However, the precise underlying mechanisms are still largely unclear. In the present study, we investigated the metabolite changes in A549 cells induced by EGCG in vitro utilizing liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. The result revealed 33 differentially expressed metabolites between untreated and 80 μM EGCG-treated A549 cells. The altered metabolites were involved in the metabolism of glucose, amino acid, nucleotide, glutathione, and vitamin. Two markedly altered pathways, including glycine, serine and threonine metabolism and alanine, aspartate and glutamate metabolism, were identified by MetaboAnalyst 5.0 metabolic pathway analysis. These results may provide potential clues for the intramolecular mechanisms of EGCG’s effect on A549 cells. Our study may contribute to future molecular mechanistic studies of EGCG and the therapeutic application of EGCG in cancer management.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LC–MS Based Metabolomics Study of the Effects of EGCG on A549 Cells

Pan

Han

et al. 2021

Front. Pharmacol.

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“…The LC–MS/MS experimental procedures were well documented in our previous studies [ 20 , 80 – 85 ]. Briefly, all LC–MS/MS experiments were performed on a Waters Aquity I-Class UPLC-XenoTQ-S micro (Waters, Milford, Massachusetts, USA) system.…”

Section: Methodsmentioning

confidence: 99%

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis

et al. 2021

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Male germ cell (GC) production is a metabolically driven and apoptosis-prone process. Here, we show that the glucose-sensing transcription factor (TF) MAX-Like protein X (MLX) and its binding partner MondoA are both required for male fertility in the mouse, as well as survival of human tumor cells derived from the male germ line. Loss of Mlx results in altered metabolism as well as activation of multiple stress pathways and GC apoptosis in the testes. This is concomitant with dysregulation of the expression of male-specific GC transcripts and proteins. Our genomic and functional analyses identify loci directly bound by MLX involved in these processes, including metabolic targets, obligate components of male-specific GC development, and apoptotic effectors. These in vivo and in vitro studies implicate MLX and other members of the proximal MYC network, such as MNT, in regulation of metabolism and differentiation, as well as in suppression of intrinsic and extrinsic death signaling pathways in both spermatogenesis and male germ cell tumors (MGCTs).

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“…The LC-MS/MS experimental procedures were well documented in our previous studies (Zhu et al, 2014) (Barton et al, 2015) (Carroll et al, 2015) (Gu et al, 2015) (Gu et al, 2016;He et al, 2020;Shi et al, 2019)). Briefly, all LC-MS/MS experiments were performed on a Waters Aquity I-Class UPLC-XenoTQ-S micro (Waters, MA) system.…”

Section: Serum Sample Preparation and Lc-ms/ms Measurementmentioning

confidence: 99%

MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis

Carroll

Cheng

Freie

et al. 2020

Preprint

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Male germ cell production is a metabolically-driven and apoptosis-prone process. Here we show that the glucose-sensing transcription factor MLX, and its binding partner MondoA, are both required for male fertility in the mouse, as well as survival of human tumor cells derived from the male germ line. Loss of Mlx results in altered metabolism as well as activation of multiple stress pathways and germ cell apoptosis in the testes. This is concomitant with dysregulation of the expression of male-specific germ cell transcripts and proteins, manifesting as oligoasthenoteratozoospermia (OAT). Our genomic and functional analyses identify loci directly bound by MLX involved in these processes, including metabolic targets, apoptotic effectors and obligate components of male-specific germ cell development. These in vivo and in vitro studies implicate MLX and other members of the proximal MYC network, such as MNT, in regulation of metabolism and differentiation, as well as in suppression of intrinsic and extrinsic death signaling pathways in both spermatogenesis and male germ cell tumors.Highlights-The MAX-like bHLHLZ protein MLX is required for male fertility, but not embryonic development.-MLX and its heterodimeric partner MondoA are each required for both male fertility and survival of male germ cell tumors.-Genomic analysis identifies direct MLX targets associated with metabolism, stress and male germ cell development.-Loss of MLX alters MYC network genome occupancy and transcriptional output.

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2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) induces wide metabolic changes including attenuated mitochondrial function and enhanced glycolysis in PC12 cells

Cited by 31 publications

References 81 publications

LC–MS Based Metabolomics Study of the Effects of EGCG on A549 Cells

LC–MS Based Metabolomics Study of the Effects of EGCG on A549 Cells

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis

MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis

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