1α,25-dihydroxyvitamin D3 stimulation of osteopontin expression in rat clonal dental pulp cells

Nagata, Toshihiko; Yokota, Mika; Ohishi, Keiji; Nishikawa, Shingo; Shinohara, Hiroyuki; Wakano, Yoichi; Ishida, Hiroshi

doi:10.1016/0003-9969(94)90007-8

Cited by 23 publications

(17 citation statements)

References 33 publications

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“…Although many clinical studies have shown the presence of pulp stones, the pathogenesis is poorly understood. We previously reported that dental pulp cells produced osteopontin (OPN), its expression was regulated by osteotrophic factors (1)(2)(3), and it localized in the human pulp stone matrix (4). OPN is a phosphorylated glycoprotein that is expressed by a broad range of tissues and cells (5).…”

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confidence: 99%

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High Glucose Levels Increase Osteopontin Production and Pathologic Calcification in Rat Dental Pulp Tissues

Inagaki

Yoshida

Ohba

et al. 2010

Journal of Endodontics

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confidence: 99%

“…RPC-C2A produced phosphorylated OPN (1), and its expression was regulated by 1,25 dihydroxyvitamin D 3 and retinoic acid (2,3). On the other hand, Otsuka Long-Evans Tokushima Fatty rats (OLETF) are an inbred strain with obesity showing the late onset of spontaneous hyperglycemia in males and are recognized as a typical animal model of type 2 diabetes (22).…”

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High Glucose Levels Increase Osteopontin Production and Pathologic Calcification in Rat Dental Pulp Tissues

Inagaki

Yoshida

Ohba

et al. 2010

Journal of Endodontics

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“…The hybridized RNA was located by exposure to Fuji RX film at -80°C for 2 h with intensifying screens (DuPont). The same membranes were reprobed with cDNAs of mouse OPN and human glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [10]. Sizes of mRNAs were estimated with a 0.249.5 kb RNA ladder (Gibco BRL).…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…1, 2 and 4). Nagata et al [10] reported that the elevated level of OPN mRNA induced by 1,25(OH)2D3 was associated with increased synthesis of OPN protein in RPC-C2A cells. Although 1,25(OH)2D3 increased the expressions of TSP and OPN with similar time courses (Fig.…”

Section: Gapdh ;mentioning

confidence: 99%

“…TSP, thrombospondin; OPN, osteopontin; TGF-]], transforming growth factor-[3; 1,25(OH)2D3. lc~,25-dihydroxyvitamin D3 ; PDD, 4~-phorbol 12,13-didecanoate; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; EMEM, Eagle's minimum essential medium; PKC, protein kinase C; SDS-PAGE, SDS polyacrylamide gel electrophoresis; VDRE, vitamin D response element thesis of osteopontin (OPN), a phosphoprotein presumably related to mineralization, in rat dental pulp-derived clonal cells (RPC-C2A cell) [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Osteotropic factor‐stimulated synthesis of thrombospondin in rat dental pulp cells

Islam¹,

Ueno²,

Nishikawa³

et al. 1996

FEBS Letters

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The amount of thrombospondin (TSP) mRNA in confluent clonal rat dental pulp cells was increased by transforming growth factor-[3 (TGF-[3), lo~,25-dihydroxyvitamin D3 (1,25(OH)2D3), and phorbol 12,13-didecanoate (PDD), with maximal levels 6, 36 and 2 h, respectively, after stimulation. These increases were accompanied by enhanced syntheses of TSP proteins which were found in the different forms in cell layer/ matrix fraction (198 and 165 kDa TSP) and the culture medium (180 kDa TSP). These three factors also raised the mRNA level of osteopontin, which is thought to play an important role in mineralization in dentin and bone. The order of effectiveness of these factors was PDD>TGF-[~> 1,25(OH)2D3 for all the stimulations described above. These results suggest that the osteotropic factors enhance TSP synthesis at the pretranslational level and that TSP produced by dental pulp cells participates in formation of reparative dentin.

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Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2

Feng

Wang

et al. 2010

Journal Cellular Physiology

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Bone morphogenetic protein 2 (Bmp2) is essential for odontogensis and dentin mineralization. Generation of floxed Bmp2 dental mesenchymal cell lines is a valuable application for studying the effects of Bmp2 on dental mesenchymal cell differentiation and its signaling pathways during dentinogenesis. Limitation of the primary culture of dental mesenchymal cells has led to the development of cell lines that serve as good surrogate models for the study of dental mesenchymal cell differentiation into odontoblasts and mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines, which were isolated from 1st mouse mandibular molars at postnatal day 1 and immortalized with pSV40 and clonally selected. These transfected cell lines were characterized by RT-PCR, immunohistochemistry, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized cell lines, iBmp2-dp, displayed a higher proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary cells as determined by expression of tooth-specific markers as well as demonstrated the ability to differentiate and form mineralized nodules. In addition, iBmp2-dp cells were inducible and responded to BMP2 stimulation. Thus, we for the first time described the establishment of an immortalized mouse floxed Bmp2 dental papilla mesenchyma cell line that might be used for studying the mechanisms of dental cell differentiation and dentin mineralization mediated by Bmp2 and other growth factor signaling pathways. Tooth development involves sequential and reciprocal interactions between dental epithelial and mesenchymal cells, and proceeds through a series of cytodifferentiations in specific spatialtemporal patterns (Linde and Goldberg, 1993). Dentinogenesis is a complex process in which multiple signaling pathways converge to induce dentin formation and is controlled by many growth and transcription factors (Thesleff, 2003). The bone morphogenetic proteins (Bmps) are structurally related to the transforming growth factor beta (TGF-β) superfamily and were originally identified by their capacity to induce ectopic bone formation in rodents (Urist, 1965;Wozney et al., 1988). Members of the Bmp family have diverse biological functions during embryonic development (Hogan, 1996;Wu et al., 2003) including a vital role in osteogenesis (Chen et al., 2004;Rosen, 2009). Among the Bmp family members, Bmp2 has been extensively studied for its various biological functions during chondrogenic and osteogenic differentiation (Reddi, 1997;Ducy and Karsenty, 2000). Also, Bmp2 has been shown to promote dental pulp stem cell commitment to the odontoblast lineage in vitro ) and induces dental pulp cell differentiation and mineralization in vitro and in vivo (Nakashima, 2005;Chen et al., 2008). However, detail understandings of the molecular mechanisms of Bmp2 exerting its effects on tooth development and formation remain elusive in particular duri...

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1α,25-dihydroxyvitamin D3 stimulation of osteopontin expression in rat clonal dental pulp cells

Cited by 23 publications

References 33 publications

High Glucose Levels Increase Osteopontin Production and Pathologic Calcification in Rat Dental Pulp Tissues

High Glucose Levels Increase Osteopontin Production and Pathologic Calcification in Rat Dental Pulp Tissues

Osteotropic factor‐stimulated synthesis of thrombospondin in rat dental pulp cells

Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2

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