1α,25-Dihydroxyvitamin D3 modulates the murine antibody response to pneumococcal capsular polysaccharide serotype 3 through IL-12

Abstract

1a, 25(OH) 2 D 3 ] is a steroid hormone that regulates calcium metabolism. Besides, 1a,25(OH) 2 D 3 also has pronounced immunomodulatory effects: it strongly inhibits dendritic cell (DC) maturation and impairs IL-12 production. We studied the effect of 1a,25(OH) 2 D 3 on the antibody response to pneumococcal capsular polysaccharide (caps-PS) serotype 3. 1a,25(OH) 2 D 3 inhibited the IgG2a antibody response to caps-PS serotype 3. Besides, 1a,25(OH) 2 D 3 also inhibited IL-12 production and maturation of DC. Anti-IL-12 and exogenous IL-12, respectively, inhibited and stimulated the IgG2a antibody response to caps-PS serotype 3. Exogenous IL-12 abrogated the effect of 1a,25(OH) 2 D 3 on the IgG2a antibody response to caps-PS serotype 3, indicating that the effect of 1a,25(OH) 2 D 3 on the IgG2a antibody response to caps-PS serotype 3 was mediated through IL-12.In conclusion, we demonstrate that 1a,25(OH) 2 D 3 has an inhibitory effect on the IgG2a antibody response to caps-PS serotype 3, and that this effect was mediated trough IL-12.

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