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doi:10.1002/dneu.v69:10

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“…SR101 use is further limited by its uptake being age-dependent and brain-region specific, 34,35 and SR101 has also been shown to interfere with plastic processes. 36 Astrocytes have also been labeled using several virus labeling strategies, 15,16,37,38 although studies of astrocytic processes in viral labeled astrocytes have been limited to ex vivo preparations. 15,16 The downside of the virus labeling approach is that injection of the virus into the brain can cause damage and inflammation which may alter the course of the experiment.…”

Section: Imaging Astrocytes and Blood Vessels In The Intact Brainmentioning

confidence: 99%

Characterization of the BAC Id3-enhanced green fluorescent protein transgenic mouse line forin vivoimaging of astrocytes

2014

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Astrocytes are highly ramified glial cells with critical roles in brain physiology and pathology. Recently, breakthroughs in imaging technology have expanded our understanding of astrocyte function in vivo. The in vivo study of astrocytic dynamics, however, is limited by the tools available to label astrocytes and their processes. Here, we characterize the bacterial artificial chromosome transgenic Id3-EGFP knock-in mouse to establish its usefulness for in vivo imaging of astrocyte processes. Using fixed brain sections, we observed enhanced green fluorescent protein expression in astrocytes and blood vessel walls throughout the brain, although the extent and cell type specificity of expression depended on the brain area and developmental age. Using in vivo two-photon imaging, we visualized astrocytes in cortical layers 1-3 in both thin skull and window preparations. In adult animals, astrocytic cell bodies and fine processes could be followed over many hours. Our results suggest that Id3 mice could be used for in vivo imaging of astrocytes and blood vessels in development and adulthood.

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