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Abstract

In this study, we analyzed 70 patients with worsening multiple sclerosis despite pharmacologic treatment who were treated with several intrathecal injections of peripheral blood cells harvested by apheresis after granulocyte-colony stimulating factor treatment. Thirty-seven patients (52%) had a reduction of Expanded Disability Status Scale score; 10 patients had relapses, although these were milder than usual and more easily controlled by corticosteroids. Because mesenchymal cells increase in the peripheral blood after granulocyte-colony stimulating factor stimulation, a peripheral blood harvest seems easier and less costly than mesenchymal cell cultivation before injection. This seems to be a reasonable treatment for progressive multiple sclerosis.

Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients

“…However, in the experiment, intra-parenchymal injection was used, which resulted in BBB damage at the site of injection, potentially skewing these results. Intranasal [39][40][41][42] and intrathecal [36,43] routes have been used for intracerebral delivery of different stem cell types with variable outcomes. At the very least, with intrathecal administration, the cells layering by gravity to the bottom of the thecal sac, as well as the normal CSF circulatory route inhibiting cells from entering the deep brain parenchyma, are problematic.…”