1H-NMR studies of lipid extracts of rat liver mitochondria

Pollesello, Piero; Eriksson, Ove; Kvam, Bjarne J.; Paoletti, Sergio; Saris, Nils‐Erik L.

doi:10.1016/0006-291x(91)91904-q

Cited by 18 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No significant change was observed in PS (peak 3), CL (peak 9) or SPM (peak 6). The PC: PE ratio of 1.6 +0.1 in control preparations was similar to that obtained by Pollesello et al [25] using 'H-NMR, with the decrease to 0.8 + 0.1I in the infected state emphasizing the differential loss of these two major phospholipids. Overall, these changes represent a loss of approximately 40 % of total phospholipid.…”

Section: Phosphoilpid Composition Of the Mitochondrial Membranessupporting

confidence: 87%

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

Lenton

Behm

Bygrave

1995

Biochemical Journal

View full text Add to dashboard Cite

The non-esterified fatty acid (NEFA) content and phospholipid composition of mitochondria isolated from the livers of Wistar rats infected with Fasciola hepatica were examined in relation to the aberrant mitochondrial respiration previously reported [Rule, Behm, and Bygrave (1989) Biochem. J. 260, 517-523]. At 2 weeks post-infection, elevated NEFA levels were associated with uncoupling of mitochondrial respiration that was reversible in vitro by the addition of BSA. State IV respiration rates showed a strong correlation with NEFA content. At 3 weeks post-infection, NEFA content had increased further and uncoupled mitochondria no longer showed any response to BSA. 31P-NMR analyses of cholate extracts of mitochondria from infected livers at 3 weeks post-infection revealed a marked loss of several major phospholipid species with a concomitant increase in catabolic products, particularly glycerophosphocholine and glycerophosphoethanolamine. Similar changes were observed in microsomal extracts. The NEFA content and phospholipid composition of mitochondria isolated from infected, athymic nude rats were not significantly different from uninfected, athymic rats. These findings suggest that uncoupling of liver mitochondria during infection with F. hepatica is the result of phospholipase activation mediated by the immune system of the host.

show abstract

Section: Phosphoilpid Composition Of the Mitochondrial Membranessupporting

confidence: 87%

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

Lenton

Behm

Bygrave

1995

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…During data processing, line broadening of 0.3 Hz was applied as a noise filter. Peaks were assigned by comparison with literature data (21,(23)(24)(25)(26). Each proton on the fatty acyl chain has its own chemical shift determined by its binding character, and its assignment is shown on the spectrum in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Changes in liver fatty acid unsaturation after partial hepatectomy in the rat

Kishino

Tanno

Yamada

et al. 2000

Lipids

View full text Add to dashboard Cite

The purpose of this study was to assess changes in the degree of fatty acid unsaturation in rat liver after partial hepatectomy. This is the first study in which liver fatty acid unsaturation has been analyzed over a long period of regeneration until day 28 after operation. The relationship between changes in unsaturation and fatty acid composition in the regenerating liver were also investigated in this study. Proton nuclear magnetic resonance spectroscopy revealed significantly elevated levels of unsaturation with a maximum on day 5 after partial hepatectomy, compared with untreated controls (11.72+/-0.55 vs. 11.05+/-0.26%, P < 0.05). No significant changes in unsaturation were found in day 1 regenerating liver, which is rich in absolute amounts of fatty acids. Based on gas-liquid chromatography, the relative amounts of oleic acid (18:1n-9) and linoleic acid (LA; 18:2n-6) were increased, while polyunsaturated fatty acids such as arachidonic acid (20:4n-6) and docosahexaenoic acid (DHA; 22:6n-3) were decreased on day 1. On the other hand, on day 5 of regeneration, while most fatty acids were returning to their preoperative control levels, only DHA was higher than the control value (7.69+/-0.58 vs. 5.57+/-0.37%, P < 0.001). The high levels of unsaturation on day 5 were found to be partly due to the increase in DHA. The findings suggest that some significant signals are transmitted during the regeneration process owing to alterations in the membrane structure by the high levels of fatty acid unsaturation and the increase in DHA levels on day 5 after partial hepatectomy.

show abstract

“…The early phase of oxidative degradation of polyunsaturated fatty acids was analysed using "H-NMR by measuring the amplitudes of methine proton signals attributable to intrachain and enol ether double bonds. This method has previously been applied to assess qualitative changes induced by the oxidation of fatty acids [11,12]. In parallel, oxidative degradation was also monitored by assessing the disappearance of the oxidizable substrate using TLC.…”

Section: Introductionmentioning

confidence: 99%

“…The purity of all commercially obtained phospholipids was assessed by one-dimensional TLC according to procedure 1 (see below). 1-Palmitoyl-2- [5,6,8,9,11,12,14,15-$H]-arachidonoyl PC was synthesized from 1-palmitoyl-2-lyso-PC and [5,6,8,9,11,12,14,15-$H]arachidonic acid (Amersham, Braunschweig, Germany) as described in [16]. 1-Alkenyl-2-lyso-PC, 1-alkenyl-2-lyso-PE and 1-alkenyl-2-acyl-PE were prepared from bovine heart PC and from bovine brain PE by alkaline methanolysis [17].…”

Section: Introductionmentioning

confidence: 99%

Delayed oxidative degradation of polyunsaturated diacyl phospholipids in the presence of plasmalogen phospholipids in vitro

Reiss

Beyer

Engelmann

1997

Biochemical Journal

140

114

View full text Add to dashboard Cite

The oxidative degradation of plasmalogen (alkenylacyl) phospholipids was analysed in the absence and the presence of polyunsaturated ester phospholipids by "H-NMR and by chemical determination. Brain lysoplasmenylethanolamine (lyso-P-PE), brain P-PE and erythrocyte P-PE, containing an increasing number of intrachain double bonds at sn # , were oxidized with 2,2h-azobis-(2-amidinopropane hydrochloride) (AAPH ; 2 or 10 mM) in Triton X-100 micelles (detergent\phospholipid 1 : 5, mol\mol). The formation of two peroxyl radicals was accompanied by the degradation of approx. one molecule of brain lyso-P-PE. On oxidation of brain P-PE or erythrocyte P-PE (320 nmol) with 2 mM AAPH, the (α-vinyl) methine "H signal of the enol ether decreased more rapidly than the methine proton peak of intrachain double bonds. The rate of enol ether degradation increased in the order : erythrocyte P-PE brain P-PE brain lyso-P-PE. The disappearance of the polyunsaturated ester phospholipids 1-palmitoyl-2-arachidonoyl phosphatidylcholine (16 : 0\20 : 4-PC) and 1-palmitoyl-2-linoleoyl phosphatidylcholine

show abstract

1H-NMR studies of lipid extracts of rat liver mitochondria

Cited by 18 publications

References 14 publications

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

Changes in liver fatty acid unsaturation after partial hepatectomy in the rat

Delayed oxidative degradation of polyunsaturated diacyl phospholipids in the presence of plasmalogen phospholipids in vitro

Contact Info

Product

Resources

About