1H NMR-metabolomics: Can they be a useful tool in our understanding of cardiac arrest?

Chalkias, Athanasios; Fanos, Vassilios; Noto, Antonio; Castrèn, Maaret; Gulati, Anil Kumar; Svavarsdóttir, Hildigunnur; Iacovidou, Nicoletta; Xanthos, Theodoros

doi:10.1016/j.resuscitation.2014.01.025

Cited by 11 publications

(11 citation statements)

References 54 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Currently animal models of neonatal hypoxia offer precise physiological measurements that can be directly compared to acute changes in the urine metabolome in order to establish a model of neonatal hypoxic injury [39]. Other studies used NMR metabolomics in a newborn piglet model, in conjunction with physiological measurements, to establish a metabolomic profile of neonatal hypoxiareoxygenation [40] for identifying the unknown biological pathways that lead to cardiac arrest [41]. In this study, by using NMR metabolomic approach, the urine of asphyctic together with control group neonates was analyzed.…”

Section: Resultsmentioning

confidence: 99%

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Longini

Giglio

Perrone

et al. 2015

Clinica Chimica Acta

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Longini

Giglio

Perrone

et al. 2015

Clinica Chimica Acta

View full text Add to dashboard Cite

“…The metabolites comprising the metabolome are end products of cellular activities and give a snapshot of physiological and pathophysiological processes. During asphyxia and resuscitation normal homeostasis is severely disturbed with profound effects on metabolism [ 6 ]. Previous studies of metabolic profiles during resuscitation of newborn piglets have demonstrated that the use of pure oxygen is associated with biochemical markers of delayed cellular recovery and increased oxidative stress [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Plasma and Urine Metabolomics in Identifying New Biomarkers in Severe Newborn Asphyxia: A Study of Asphyxiated Newborn Pigs following Cardiopulmonary Resuscitation

et al. 2016

View full text Add to dashboard Cite

BackgroundOptimizing resuscitation is important to prevent morbidity and mortality from perinatal asphyxia. The metabolism of cells and tissues is severely disturbed during asphyxia and resuscitation, and metabolomic analyses provide a snapshot of many small molecular weight metabolites in body fluids or tissues. In this study metabolomics profiles were studied in newborn pigs that were asphyxiated and resuscitated using different protocols to identify biomarkers for subject characterization, intervention effects and possibly prognosis.MethodsA total of 125 newborn Noroc pigs were anesthetized, mechanically ventilated and inflicted progressive asphyxia until asystole. Pigs were randomized to resuscitation with a FiO2 0.21 or 1.0, different duration of ventilation before initiation of chest compressions (CC), and different CC to ventilation ratios. Plasma and urine samples were obtained at baseline, and 2 h and 4 h after return of spontaneous circulation (ROSC, heart rate > = 100 bpm). Metabolomics profiles of the samples were analyzed by nuclear magnetic resonance spectroscopy.ResultsPlasma and urine showed severe metabolic alterations consistent with hypoxia and acidosis 2 h and 4 h after ROSC. Baseline plasma hypoxanthine and lipoprotein concentrations were inversely correlated to the duration of hypoxia sustained before asystole occurred, but there was no evidence for a differential metabolic response to the different resuscitation protocols or in terms of survival.ConclusionsMetabolic profiles of asphyxiated newborn pigs showed severe metabolic alterations. Consistent with previously published reports, we found no evidence of differences between established and alternative resuscitation protocols. Lactate and pyruvate may have a prognostic value, but have to be independently confirmed.

show abstract

“…We used metabolomics to provide a systematic study of the polar metabolites (metabolome) present in the cardiomyocyte at the time of her death. There are reported end-point metabolites of biological events after cardiac arrest and resuscitation [8]. In this pilot study, the metabolomics approach offered insight into the cardiac molecule regulation and signaling at the time of our patient’s presumed cardiac death.…”

Section: Discussionmentioning

confidence: 99%

Cardiac metabolomics and autopsy in a patient with early diffuse systemic sclerosis presenting with dyspnea: a case report

et al. 2015

View full text Add to dashboard Cite

IntroductionDiffuse systemic sclerosis is associated with high mortality; however, the pathogenesis of cardiac death in these patients is not clear.Case presentationA 56-year-old Caucasian female patient presented with dyspnea and requested to donate her body to science in order to improve understanding of diffuse systemic sclerosis pathogenesis. She had extensive testing for dyspnea including pulmonary function tests, an echocardiogram, cardiac magnetic resonance imaging, and right heart catheterization to characterize her condition. Her case highlights the morbidity seen in this disease, including the presence of extensive skin thickening, digital ulcerations, and scleroderma renal crisis.ConclusionIn this case report, we present the finding of cardiac tissue metabolomics, which may indicate a problem with vasodilation as a contributor to cardiac death in diffuse systemic sclerosis. The use of autopsy and tissue metabolomics in rare disease may help clarify disease pathogenesis.

show abstract

1H NMR-metabolomics: Can they be a useful tool in our understanding of cardiac arrest?

Cited by 11 publications

References 54 publications

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

The Role of Plasma and Urine Metabolomics in Identifying New Biomarkers in Severe Newborn Asphyxia: A Study of Asphyxiated Newborn Pigs following Cardiopulmonary Resuscitation

Cardiac metabolomics and autopsy in a patient with early diffuse systemic sclerosis presenting with dyspnea: a case report

Contact Info

Product

Resources

About