1H NMR metabolomic approach reveals chlorogenic acid as a response of sugarcane induced by exposure to Diatraea saccharalis

Sabino, Adilson Rodrigues; Tavares, Sheila S.; Riffel, Alessandro; Li, Jia V.; Oliveira, Demetrios José Albuquerque; Feres, Chrystian I.M.A.; Henrique, Lucas; Oliveira, Josiane Silva de; Correia, Gonçalo dos Santos; Sabino, Anderson Rodrigues; Nascimento, Ticiano Gomes do; Hawkes, Geofrey; Santana, Antônio Euzébio Goulart; Holmes, Elaine; Bento, Edson S.

doi:10.1016/j.indcrop.2019.111651

Cited by 16 publications

(9 citation statements)

References 23 publications

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“…Under the hypothesis that the level of field resistance rather depends on induced changes by MCB attack than on constitutive defenses and those changes are determined by the duration of insect feeding, ions impli-cated in the long term response could be important determinants of genotype induced resistance. Agreeing with other studies on induced metabolic plant changes by insect attack, the primary metabolism was downregulated in both maize genotypes by MCB 9-day feeding, including sugar, amino acid fatty acid and vitamin metabolism, as well as the tricarboxylic acid (TCA) or Krebs cycle which implies a disruption of energy production (Kang et al , 2019, Liu et al , 2010, Sabino, Tavares, Riffel, Li, Oliveira, Feres, Henrique, Oliveira, Correia, Nascimento, Hawkes, Santana, Holmes & Bento, 2019, Wanget al , 2016. According to a general downregulation of energy production and metabolism, intermediates or end-products of the shikimate pathway were repressed by MCB infestation in the susceptible inbred since decreases of tryptophan, phenylalanine and beta-tyrosine levels and those of several phenolic and indole-related compounds derived from them were registered in that inbred after 9-day feeding.…”

Section: Discussionsupporting

confidence: 63%

Maize Resistance to Stem Borers can be modulated by Systemic Maize Responses to Long-term Stem Tunneling

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Scarce attention has been paid to maize (Zea mays L.) resistance induced by corn borer damage although evidence shows that induced defenses have lower resource allocation costs than constitutive defenses. The maize responses to short and long-term attacks by the Mediterranean corn borer (MCB, Sesamia nionagrioides) have been previously studied but suggested differences between responses could be due to experimental differences. Therefore, in the current study, a direct comparison between short and long-term responses has been made. The objectives were (i) to determine changes in the level of antibiosis of the stems induced by feeding of Sesamia nonagrioides (Mediterranean Corn Borer; MCB) larvae for two days (short-term feeding) and nine days (long-term feeding), (ii) to characterize the metabolome of the stem short and long-term responses to borer attack and (iii) to look for metabolic pathways that could modulate plant resistance to MCB. Adjustment of plant performance under subsequent conspecific attack due to previous insect damage was genotype-dependent; defenses were progressively induced in the resistant inbred and constitutive defenses were broken down in the susceptible inbred. Results suggest that the different resistance of the two inbreds to stem tunneling by MCB could depend on their ability to stablish a systemic response.

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Section: Discussionsupporting

confidence: 63%

Maize Resistance to Stem Borers can be modulated by Systemic Maize Responses to Long-term Stem Tunneling

et al.

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“…A general overview of our metabolomic results yields a downregulation of the primary metabolism in both maize genotypes by 9days of MCB feeding. This includes sugar, amino acid, fatty acid, and vitamin metabolism, as well as the tricarboxylic acid (TCA) or Krebs cycle, which implies a disruption of energy production ( Liu et al, 2010 ; Wang et al, 2016 ; Kang et al, 2019 ; Sabino et al, 2019 ). Especially remarkable is that the organic acids malate and malonate were only detected in stems of PB130 preconditioned by 9days of feeding.…”

Section: Resultsmentioning

confidence: 99%

Maize Resistance to Stem Borers Can Be Modulated by Systemic Maize Responses to Long-Term Stem Tunneling

et al. 2021

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Limited attention has been paid to maize (Zea mays L.) resistance induced by corn borer damage, although evidence shows that induced defenses have lower resource allocation costs than constitutive defenses. Maize responses to short- and long-term feeding by the Mediterranean corn borer (MCB, Sesamia nionagrioides) have been previously studied, but the suggested differences between responses could be due to experimental differences. Therefore, in the current study, a direct comparison between short- and long-term responses has been made. The objectives were (i) to determine changes in the level of antibiosis of the stems induced by feeding of S. nonagrioides larvae for 2days (short-term feeding) and 9days (long-term feeding), (ii) to characterize the metabolome of the stems’ short- and long-term responses to borer feeding, and (iii) to look for metabolic pathways that could modulate plant resistance to MCB. Defenses were progressively induced in the resistant inbred, and constitutive defenses were broken down in the susceptible inbred. Results suggest that the different resistance levels of the two inbreds to stem tunneling by MCB could depend on their ability to establish a systemic response. Based on these results, a high throughput look for specific metabolites implicated in systemic induced resistance to maize stem borers is recommended; the current focus on constitutive defense metabolites has not been successful in finding molecules that would be valuable tools for pest control.

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“…In addition, these metabolites are associated with known important medicinal properties, and their presence in M. oleifera leaf extract has been widely reported. As indicated in Table 2 , the targeted compounds were identified using the NMR chemical shifts as previously published [ 4 , 6 , 24 , 26 , 28 , 29 , 30 , 31 , 32 ], assisted by databases such as Chenomx profiler and the Human Metabolome Database ( Supplementary Table S1 ). Annotation of chlorogenic and GABA were further confirmed through spiking with commercial standards.…”

Section: Resultsmentioning

confidence: 99%

NMR-Based Metabolomic Analyses to Identify the Effect of Harvesting Frequencies on the Leaf Metabolite Profile of a Moringa oleifera Cultivar Grown in an Open Hydroponic System

2021

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Moringa oleifera Lam. is one of the world’s most useful medicinal plants. Different parts of the M. oleifera tree contain a rich profile of important minerals, proteins, vitamins, and various important bioactive compounds. However, there are differences in the phytochemical composition of the medicinal plant’s raw materials due to seasonal variation, cultivation practices, and post-harvest processing. The main objective of this study was therefore to determine the effect of harvesting frequencies on selected bioactive compounds of a M. oleifera cultivar (PKM1) grown in a hydroponic system under a shade net structure. Three harvesting frequency treatments were applied in the study, with the plants harvested at every 30 days (high frequency), 60 days (intermediate frequency), and 90 days (low frequency) respectively. 1H-NMR was used for data acquisition, and multivariate data analysis by means of principal component analysis (PCA), partial least square discriminatory analysis (PLS-DA), and orthogonal partial least square discriminatory analysis (OPLS-DA) were applied to determine the changes in the leaf metabolite profile, and also to identify the spectral features contributing to the separation of samples. Targeted metabolite analysis was used to match the NMR peaks of the compounds with the NMR chemical shifts of the contribution plot. The contribution plot showed that the increase in concentration of some compounds in aliphatic, sugar and aromatic regions contributed to the separation of the samples. The results revealed that intermediate and low harvesting frequencies resulted in a change in the leaf metabolite profile. Compounds such as chlorogenic acid, ferulic acid, vanillic acid, wogonin, esculetin, niazirin, and gamma-aminobutyric acid (GABA) showed an increase under intermediate and low harvesting frequencies. These results provide insight into the effect of harvesting frequencies on the metabolite profile and associated medicinal activity of M. oleifera.

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1H NMR metabolomic approach reveals chlorogenic acid as a response of sugarcane induced by exposure to Diatraea saccharalis

Cited by 16 publications

References 23 publications

Maize Resistance to Stem Borers can be modulated by Systemic Maize Responses to Long-term Stem Tunneling

Maize Resistance to Stem Borers can be modulated by Systemic Maize Responses to Long-term Stem Tunneling

Maize Resistance to Stem Borers Can Be Modulated by Systemic Maize Responses to Long-Term Stem Tunneling

NMR-Based Metabolomic Analyses to Identify the Effect of Harvesting Frequencies on the Leaf Metabolite Profile of a Moringa oleifera Cultivar Grown in an Open Hydroponic System

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