1H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant

Rahimi, Ruhollah; Farahmand, Hamid; Mirvaghefi, Alireza; Rafiee, Gholamreza; Abed‐Elmdoust, Amirreza

doi:10.1007/s10695-019-00615-8

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…Studies have shown that different compounds produced different resonance frequencies in the magnetic field, and a chemical shift occurs. According to this principle, MRS can distinguish different compounds in the frequency axis, and use chemical shift and J-coupling to convert the acquired information into a spectrum, the horizontal axis represents the chemical shift, and the vertical axis peak represents the signal intensity, while the peak of the spectrum The area is proportional to the concentration of the species that produces the peak, which is the basis for structural inference and quantitative analysis of the corresponding compounds by MRS [14]. Although MRS cannot be continuously measured the concentration of the compound, it has the following characteristics: completely non-invasive, accurate positioning, high sensitivity, and quantitative analysis of tissue metabolic changes at the molecular level by imaging technology [15].…”

Section: Methodsmentioning

confidence: 99%

Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model

et al. 2019

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BackgroundDimethyl sulfoxide (DMSO) cryoprotectant can effectively alleviate the damage to single tissue during cryopreservation and restore its physiological activity after rewarming. However, studies have not been successful for preserving large tissue. This study aimed to investigate the application conditions of DMSO in large composite-tissue by performing femoral artery perfusion and soaking in a rabbit hind-limb model.Material/MethodsA microdialysis-freezing point osmometer was used to detect the minimum time required for effective perfusion of 10% v/v perfusion and 20% v/v perfusion group. Magnetic resonance spectroscopy (MRS) was used to detect the area under the spectrum peak of DMSO in perivascular, intramuscular, subcutaneous areas, and compare the area under the spectrum peak in the 20% vascular perfusion group and other whole immersion groups.ResultsThe minimum time required for effective perfusion of muscle in the 10% v/v group was 30 minutes, the DMSO concentration was stable at 5% v/v; In the 20% v/v group the minimum time was at 20 minutes, stable at 12% v/v. There was a statistically difference of the area under the spectrum peak in the 10% group and the 20% v/v group after vascular perfusion in different tissue locations (P<0.05). The 20% vascular perfusion group and the different concentration of DMSO in the large tissue soaking group were statistically different (P<0.05). There was a significant difference in the 20% v/v vascular perfusion group compared to the low concentration immersion group, but no difference compared to the 50% immersion group.ConclusionsThe effect of blood perfusion on cryopreservation in large tissue by using DMSO was slightly better than overall soaking, especially in preservation of skin and subcutaneous tissue.

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Section: Methodsmentioning

confidence: 99%

Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model

et al. 2019

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“…Few studies have been performed on the role of cyclodextrins in different freeze-thawing protocols for fish semen (Table 4). Rahimi et al (2015Rahimi et al ( , 2019 reported that HbCD had protective effects on cryopreserved sperm from the Persian sturgeon. They recommended that 10 mM of HβCD be used as an additive cryoprotectant for increasing cryopreserved semen quality in this species.…”

Section: In Fishmentioning

confidence: 99%

“…Rahimi et al. (2015, 2019) reported that HbCD had protective effects on cryopreserved sperm from the Persian sturgeon. They recommended that 10 mM of HβCD be used as an additive cryoprotectant for increasing cryopreserved semen quality in this species.…”

Section: Non‐permeable Cryoprotectant Agentsmentioning

confidence: 99%

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

Aramli,

Sarvi Moghanlou,

Pourahad Anzabi

2024

Reprod Domestic Animals

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Cryopreservation is a valuable technique used to assist in the genetic improvement of cultured stocks and provide a continuous supply of good‐quality semen for artificial insemination. Conserving semen by cryopreservation serves several purposes (e.g. artificial reproductive technologies and species conservation) and is also used in the clinical treatment of human infertility. However, the lifespan of cryopreserved semen is influenced by a range of factors, including storage temperature, cooling rate, chemical composition of the extender, the concentration of cryoprotectant, reactive oxygen species, seminal plasma composition and hygienic control. The choice of cryoprotectant is a vital factor underlying the success of animal semen cryopreservation. In this regard, extensive research has been carried out on various cryoprotectants, such as egg yolk, dimethyl sulfoxide, methanol, ethylene glycol and dimethylacetamide. Recent studies have also described the use of a range of new cryoprotectants for cryopreservation, including compounds of plant origin (soy), amino acids, antifreeze proteins, carbohydrates and cyclodextrins. Moreover, semen cryopreservation and storage require the use of liquid nitrogen or ultralow refrigeration methods for both long‐ and short‐term storage. This review summarizes the general methods used for freezing semen and discusses the use of traditional and newly emerging cryoprotectants (permeable and non‐permeable) for the cryopreservation of semen in selected fish and mammalian species.

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Metabolomics in sturgeon research: a mini-review

Liu,

Naganuma

2024

Fish Physiol Biochem

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Sturgeons are ancient fish, with 27 species distributed in the Northern Hemisphere. This review first touches upon the significance of sturgeons in the context of their biological, ecological, and economic importance, highlighting their status as “living fossils” and the challenges they face in genomic research due to their diverse chromosome numbers. This review then discusses how omics technologies (genomics, transcriptomics, proteomics, and metabolomics) have been used in sturgeon research, which so far has only been done on Acipenser species. It focuses on metabolomics as a way to better understand how sturgeons work and how they react to their environment. Specific studies in sturgeon metabolomics are cited, showing how metabolomics has been used to investigate various aspects of sturgeon biology, such as growth, reproduction, stress responses, and nutrition. These studies demonstrate the potential of metabolomics in improving sturgeon aquaculture practices and conservation efforts. Overall, the review suggests that metabolomics, as a relatively new scientific tool, has the potential to enhance our understanding of sturgeon biology and aid in their conservation and sustainable aquaculture, contributing to global food security efforts.

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1H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant

Cited by 6 publications

References 48 publications

Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model

Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

Metabolomics in sturgeon research: a mini-review

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