1H NMR-based metabolomics investigation of Daphnia magna responses to sub-lethal exposure to arsenic, copper and lithium

Nagato, Edward G.; D’eon, Jessica C.; Lankadurai, Brian P.; Poirier, David G.; Reiner, Eric J.; Simpson, André J.; Simpson, Myrna J.

doi:10.1016/j.chemosphere.2013.04.085

Cited by 81 publications

(74 citation statements)

References 57 publications

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“…A total of 200 peaks in each sample were analyzed, and 65 metabolites were identified, as shown in Tables S1 and S2. Compared with other results from metabolic analyses (e.g., using 1 H NMR technology), the applied extraction procedure and gas chromatography-mass spectrometry/mass spectrometry with the derivatization method were effective for analyzing the multiclass metabolites of plant cells2829. These metabolites correspond to the main metabolic pathways involved in carbohydrate, amino acid and fatty acid production, secondary metabolism and the urea cycle.…”

Section: Resultsmentioning

confidence: 99%

Graphene oxide amplifies the phytotoxicity of arsenic in wheat

Kang

et al. 2014

Sci Rep

143

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Graphene oxide (GO) is widely used in various fields and is considered to be relatively biocompatible. Herein, “indirect” nanotoxicity is first defined as toxic amplification of toxicants or pollutants by nanomaterials. This work revealed that GO greatly amplifies the phytotoxicity of arsenic (As), a widespread contaminant, in wheat, for example, causing a decrease in biomass and root numbers and increasing oxidative stress, which are thought to be regulated by its metabolisms. Compared with As or GO alone, GO combined with As inhibited the metabolism of carbohydrates, enhanced amino acid and secondary metabolism and disrupted fatty acid metabolism and the urea cycle. GO also triggered damage to cellular structures and electrolyte leakage and enhanced the uptake of GO and As. Co-transport of GO-loading As and transformation of As(V) to high-toxicity As(III) by GO were observed. The generation of dimethylarsinate, produced from the detoxification of inorganic As, was inhibited by GO in plants. GO also regulated phosphate transporter gene expression and arsenate reductase activity to influence the uptake and transformation of As, respectively. Moreover, the above effects of GO were concentration dependent. Given the widespread exposure to As in agriculture, the indirect nanotoxicity of GO should be carefully considered in food safety.

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Section: Resultsmentioning

confidence: 99%

Graphene oxide amplifies the phytotoxicity of arsenic in wheat

Kang

et al. 2014

Sci Rep

143

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“…They occupy an intermediate position in the food web, are ubiquitous, highly sensitive to toxicants, easy to culture, fast growing, and have a short lifespan, making them suitable organisms for ecotoxicity tests and the analysis of aquatic ecological food webs [18,19]. Because of this, environmental metabolomic studies using D. magna and related species have increased substantially [18,20,21,22,23,24,25,26,27]. However, very little is known about the mechanisms of sub-lethal PFOS toxicity and how sub-lethal exposure alters the D. magna metabolome.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Sub-Lethal Toxicity of Perfluorooctane Sulfonate (PFOS) to Daphnia magna Using 1H Nuclear Magnetic Resonance-Based Metabolomics

et al. 2017

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1H nuclear magnetic resonance (NMR)-based metabolomics was used to characterize the response of Daphnia magna after sub-lethal exposure to perfluorooctane sulfonate (PFOS), a commonly found environmental pollutant in freshwater ecosystems. Principal component analysis (PCA) scores plots showed significant separation in the exposed samples relative to the controls. Partial least squares (PLS) regression analysis revealed a strong linear correlation between the overall metabolic response and PFOS exposure concentration. More detailed analysis showed that the toxic mode of action is metabolite-specific with some metabolites exhibiting a non-monotonic response with higher PFOS exposure concentrations. Our study indicates that PFOS exposure disrupts various energy metabolism pathways and also enhances protein degradation. Overall, we identified several metabolites that are sensitive to PFOS exposure and may be used as bioindicators of D. magna health. In addition, this study also highlights the important utility of environmental metabolomic methods when attempting to elucidate acute and sub-lethal pollutant stressors on keystone organisms such as D. magna.

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“…Ammonia is also excreted through the urine. Lithium has been shown to cause a significant decrease in glutamine in D. magna (Nagato et al, 2013) most likely via deamination of glutamine to ammonia. The [Na + ] gradient is directly connected to glutamine influx (Nobuyuki et al, 1999).…”

Section: Ion Transportmentioning

confidence: 99%

“…An investigation of lithium toxicity to Daphnia magna suggested that Li might have a mechanism analogous to that of copper: since energy production and ionoregulation were impaired (Nagato et al, 2013). One of the direct targets of Li is Mg-dependent intracellular enzymes (O'Donnell and Gould, 2007).…”

Section: Introductionmentioning

confidence: 98%