1H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus)

Liu, Yan; Chen, Ting; Li, Minghui; Xu, Hongbing; Jia, Ai‐Qun; Zhang, Jianfa; Wang, Junsong

doi:10.1016/j.chemosphere.2015.07.030

Cited by 36 publications

(20 citation statements)

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“…Color-coded loading plots were constructed to demonstrate which variables are responsible for group separation. The fold-change values of metabolites were highlighted by using color-code in colored tables as well as their p -values adjusted by the Benjamini-Hochberg method [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Wang

Guan

Yang

et al. 2020

BMC Complement Med Ther

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Background Inflammation is a response to tissue injuries, which is indispensable and important for human health, but excessive inflammation can potentially cause damage to the host organisms. Camellia nitidissima Chi, one traditional medicinal and edible plant in China, was reported to exhibit anti-inflammation capability. Hence, this study was conducted to isolate the bioactive compounds from the flowers of C. nitidissima Chi and evaluate their anti-inflammatory activity. Methods The phytochemicals from the flowers of C. nitidissima Chi were isolated and purified by silica gel, Sephadex LH-20 gel, C18 reversed silica gel, semi-preparative HPLC, and identified by the spectrum technologies. The anti-inflammatory activity of isolated compounds was evaluated using cultured macrophage RAW 264.7 cells. Whereafter the potential metabolic mechanism of the anti-inflammatory activity of the bioactive compound was investigated by a 1H-NMR based metabolomics approach. The metabolites in 1H-NMR spectra were identified by querying the Human Metabolome Database and Madison Metabolomics Consortium Database online. And the multivariate statistical analysis was performed to evaluate the variability of metabolites among samples and between sample classes. Results The compound isolated from the flowers of C. nitidissima Chi was identified as 3-cinnamoyltribuloside (3-CT). 3-CT could inhibit the NO production and the mRNA expression of iNOS involved in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Moreover, 3-CT could inhibit the expression of a series of inflammatory cytokines, including TNF-α, IL-1β, and IL-6, both at the mRNA level and protein level. The 1H-NMR based metabolomics approach was applied to investigate the potential metabolic mechanism of the anti-inflammatory activity of 3-CT. Thirty-five metabolites were identified and assigned. Orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA) of the 1H-NMR data showed 3-CT could balance the significant changes in many endogenous metabolites (e.g., choline, glucose, phenylalanine) induced by LPS in RAW 264.7 cells, which related to cholinergic anti-inflammatory pathway, oxidative stress, energy metabolism, and amino acids metabolism. Conclusion 3-CT, isolated from the flowers of C. nitidissima Chi, had potent anti-inflammatory activity in LPS-activated RAW 264.7 cells. Furthermore, our results indicated that 3-CT had effects on the cholinergic anti-inflammatory pathway, oxidative stress, energy metabolism, and amino acids metabolism in LPS-activated RAW 264.7 cells.

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Section: Methodsmentioning

confidence: 99%

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Wang

Guan

Yang

et al. 2020

BMC Complement Med Ther

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“…Whether atrazine interferes with Ar signaling is still unknown, but altered androgen metabolism was observed in fish exposed to atrazine (Moore and Waring, 1998;Spano et al, 2004). On the contrary, Dichlorvosan insecticide that shortens ceratobranchial bones in African catfish (Clarias gariepinus; Doherty et al, 2016)was found to affect Ar activity in a cell-based screening assay (Tavassoli et al, 2007), while also inhibiting acetylcholinesterase and inducing oxidative stress in vivo (Liu et al, 2015;Doherty et al, 2016).…”

Section: Androgen Receptormentioning

confidence: 99%

Fish as a model to assess chemical toxicity in bone

et al. 2018

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Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to hepato-, immune-, neuro- and/or reproductive toxicities. Nevertheless, sub-lethal effects of toxicants on skeletal development and/or bone maintenance may result in impaired growth, reduced survival rate, increased disease susceptibility and diminished welfare. Osteotoxicity may occur by acute or chronic exposure to different environmental insults. Because of biologically and technically advantagous features - easy to breed and inexpensive to maintain, external and rapid rate of development, translucent larvae and the availability of molecular and genetic tools - the zebrafish (Danio rerio) has emerged in the last decade as a vertebrate model system of choice to evaluate osteotoxicity. Different experimental approaches in fish species and analytical tools have been applied, from in vitro to in vivo systems, from specific to high throughput methodologies. Current knowledge on osteotoxicity and underlying mechanisms gained using fish, with a special emphasis on zebrafish systems, is reviewed here. Osteotoxicants have been classified into four categories according to the pathway involved in the transduction of the osteotoxic effects: activation/inhibition of membrane and/or nuclear receptors, alteration of redox condition, mimicking of bone constituents and unknown pathways. Knowledge on these pathways is also reported here as it may provide critical insights into the development, production and release of future chemical compounds with none or low osteotoxicity, thus promoting the green/environmental friendly chemistry.

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“…Furthermore, 1 H-NMR spectroscopy combined with pattern recognition and related multivariate statistical methods could offer an efficient way for assessing the metabolic functions 18,19 . It can also identify significant inherent patterns in a set of indirect measurements that classify objects combined with pattern recognition methods, such as partial least-squares discriminant analysis (PLS-DA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) 20,21 . Approaches to PLS-DA or OPLS-DA could reduce the dimensionality of complex data sets, facilitate the visualization of inherent patterns among the data set and accelerate the interpretation for various functions.…”

Section: Introductionmentioning

confidence: 99%

¹H-NMR based metabolomics reveals the nutrient differences of two kinds of freshwater fish soups before and after simulated gastrointestinal digestion

et al. 2020

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1H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus)

Cited by 36 publications

References 50 publications

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Fish as a model to assess chemical toxicity in bone

¹H-NMR based metabolomics reveals the nutrient differences of two kinds of freshwater fish soups before and after simulated gastrointestinal digestion

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1H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus)

Cited by 36 publications

References 50 publications

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Anti-inflammatory activity of 3-cinnamoyltribuloside and its metabolomic analysis in LPS-activated RAW 264.7 cells

Fish as a model to assess chemical toxicity in bone

1H-NMR based metabolomics reveals the nutrient differences of two kinds of freshwater fish soups before and after simulated gastrointestinal digestion

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¹H-NMR based metabolomics reveals the nutrient differences of two kinds of freshwater fish soups before and after simulated gastrointestinal digestion