1H NMR based metabolomic profiling of early life stage zebrafish (Danio rerio) exposed to a water-soluble fraction of weathered sediment-bound diluted bitumen

Fujita, Kaden K.; Xia, Zhe; Tomy, Gregg T.; Montina, Tony; Wiseman, Steve

doi:10.1016/j.aquatox.2021.105766

Cited by 12 publications

(4 citation statements)

References 45 publications

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“…Changes in abundance of cyp1a transcript in embryos exposed to PAHs were determined relative to the DMSO control using the efficiency‐corrected method of Pfaffl (2001). Efficiency of the cyp1a and 18s reactions was 102% and 95%, respectively (Fujita et al, 2021; Van Essen et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

Dubiel

Green

Raza

et al. 2022

Enviro Toxic and Chemistry

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Polycyclic aromatic hydrocarbons (PAHs) are structurally diverse organic chemicals that can have adverse effects on the health of fishes through activation of aryl hydrocarbon receptor 2 (AhR2). They are ubiquitous in the environment, but alkyl PAHs are more abundant in some environmental matrices. However, relatively little is known regarding the effects of alkylation on the toxicity of PAHs to fishes in vivo and how this relates to potency for activation of AhR2 in vitro. Therefore, the objectives of the present study were to determine the toxicity of benz[a]anthracene and three alkylated homologs representing various alkylation positions to early life stages of zebrafish (Danio rerio) and to assess the potency of each for activation of the zebrafish AhR2 in a standardized in vitro AhR transactivation assay. Exposure of embryos to each of the PAHs caused a dose‐dependent increase in mortality and malformations characteristic of AhR2 activation. Each alkyl homolog had in vivo toxicities and in vitro AhR2 activation potencies different from those of the parent PAH in a position‐dependent manner. However, there was no statistically significant linear relationship between responses measured in these assays. The results suggest a need for further investigation into the effect of alkylation on the toxicity of PAHs to fishes and greater consideration of the contribution of alkylated homologs in ecological risk assessments. Environ Toxicol Chem 2022;41:1993–2002. © 2022 SETAC

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Section: Methodsmentioning

confidence: 99%

Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

Dubiel

Green

Raza

et al. 2022

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

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“…The authors used a combination of 1 H-NMR for the analysis of monosaccharides, amino acids, and organic acids, GC-MS for cholesterol and fatty acids, and LC-MS for the analysis of lipids. However, more recent zebrafish toxicometabolomics studies (≥2017) reported using LC-HRMS (Orbitrap or QTOF) [15,28,29,31,33,36,48,51,[85][86][87][88], followed by 1 H-NMR [24,30,[89][90][91][92][93] and GC-HRMS [21,94], while multi-analytical platform studies remain scarce.…”

Section: Instrumental Analysismentioning

confidence: 99%

Optimization of a liquid chromatography-ion mobility-high resolution mass spectrometry platform for untargeted lipidomics and application to HepaRG cell extracts

Silva

Iturrospe

Heyrman

et al. 2021

Talanta

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“…The authors used a combination of 1 H-NMR for the analysis of monosaccharides, amino acids, and organic acids, GC-MS for cholesterol and fatty acids, and LC-MS for the analysis of lipids. However, more recent zebrafish toxicometabolomics studies (≥2017) reported using LC-HRMS (Orbitrap or QTOF) [ 15 , 28 , 29 , 31 , 33 , 36 , 48 , 51 , 85 , 86 , 87 , 88 ], followed by 1 H-NMR [ 24 , 30 , 89 , 90 , 91 , 92 , 93 ] and GC-HRMS [ 21 , 94 ], while multi-analytical platform studies remain scarce.…”

Section: Experimental Designmentioning

confidence: 99%

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

et al. 2021

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Metabolomics has achieved great progress over the last 20 years, and it is currently considered a mature research field. As a result, the number of applications in toxicology, biomarker, and drug discovery has also increased. Toxicometabolomics has emerged as a powerful strategy to provide complementary information to study molecular-level toxic effects, which can be combined with a wide range of toxicological assessments and models. The zebrafish model has gained importance in recent decades as a bridging tool between in vitro assays and mammalian in vivo studies in the field of toxicology. Furthermore, as this vertebrate model is a low-cost system and features highly conserved metabolic pathways found in humans and mammalian models, it is a promising tool for toxicometabolomics. This short review aims to introduce zebrafish researchers interested in understanding the effects of chemical exposure using metabolomics to the challenges and possibilities of the field, with a special focus on toxicometabolomics-based mass spectrometry. The overall goal is to provide insights into analytical strategies to generate and identify high-quality metabolomic experiments focusing on quality management systems (QMS) and the importance of data reporting and sharing.

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1H NMR based metabolomic profiling of early life stage zebrafish (Danio rerio) exposed to a water-soluble fraction of weathered sediment-bound diluted bitumen

Cited by 12 publications

References 45 publications

Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

Alkylation of Benz[a]anthracene Affects Toxicity to Early–Life Stage Zebrafish and In Vitro Aryl Hydrocarbon Receptor 2 Transactivation in a Position‐Dependent Manner

Optimization of a liquid chromatography-ion mobility-high resolution mass spectrometry platform for untargeted lipidomics and application to HepaRG cell extracts

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

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