1H MRS markers of tumour growth in intrasplenic tumours and liver metastasis induced by injection of HT-29 cells in nude mice spleen

Abstract

We have characterized, by in vitro magnetic resonance spectroscopy (MRS), the metabolite pattern of perchloric acid (PCA) extracts of intrasplenic tumours and hepatic metastasis, produced by intra‐spleen injection of the human colorectal carcinoma cell line HT‐29 and its metastatic variant HT‐29 MMM into nude mice. Our aim was to gain further understanding of colorectal tumour metabolism as a basis for future in vivo studies of human colon cancer by 1H MRS. Metabolite PCA extract analysis showed a good reproduction of the spectral pattern observed in human primary colon tumours, while they were very different from the spectral pattern of the host tissues (spleen and liver). The main differences between host and tumour tissues involved taurine, phosphocholine (PC), phosphoethanolamine (PE), creatine, glycogen and glucose. Creatine is the most promising marker to follow tumour growth because of its practical absence in the nude mice host tissues. Detection of variable levels of this compound and of taurine in hepatic foci in man, are suggested as possible diagnostic markers. No correlation could be found between spectral pattern differences and the different ability to metastasize of the two HT‐29 cell lines used. Furthemore, indirect evidence for a functional link between taurine and myo‐inositol in colon tumour cells is presented. In summary, our data suggest that the nude mice model may be a suitable system for the MRS study of the changes taking place in host tissues upon tumour progression. © 1998 John Wiley & Sons, Ltd.

Metabolomics by Proton Nuclear Magnetic Resonance Spectroscopy of the Response to Chloroethylnitrosourea Reveals Drug Efficacy and Tumor Adaptive Metabolic Pathways

“…The used differential metabolomics technique involving quantitative two-dimensional proton NMR spectroscopy was innovative among the other proposed quantification procedures (6,7,13,17,25,26). Because correlation signals in two-dimensional proton NMR spectroscopy are all variably related to concentration (19) and because no standardization of the signal has emerged, it remains a major aim in metabolomics to develop novel quantitative procedures.…”

“…It is presently acknowledged that nuclear magnetic resonance (NMR) spectroscopy is a first-line tool for metabolomics (1). One-dimensional proton NMR spectroscopy cumulates the advantages of being sensitive and quantitative, taking advantage of semiautomatic data processing (6). Two-dimensional NMR spectroscopy entered the field of metabolomics recently.…”

Liver Metabolite Concentrations Measured with1H MR Spectroscopy

“…Glycogen within the same volume used for 1 H MR spectroscopy could perhaps be quantified with 13 C MR spectroscopy (5) or with biopsy results (41), but neither of these Glycogen is present in glucosyl unit concentrations of about 270 mmol/ kg wet weight in human livers (41) and 110-800 mmol/kg wet weight in rabbit livers (42). Glycogen was visible in human liver tissue samples with (40). Creatine, which is synthesized in the liver, has a methyl resonance that could usually also be observed at about 3.06 ppm, but in this study, signal-tonoise ratio and resolution were insufficient to reliably quantify creatine.…”

Quantitative HRMAS proton total correlation spectroscopy applied to cultured melanoma cells treated by chloroethyl nitrosourea: Demonstration of phospholipid metabolism alterations

“…In the field of cancer, and among homonuclear 2D spectroscopy sequences, proton COSY had enabled the quantification of relative concentrations of some Plp derivatives and other species from perchloric acid extracts of colon carcinoma (19). COSY has also been used to obtain fucose:Cho ratios in several cancer cell lines (20).…”