1H and 13C nuclear magnetic resonance studies of plasma from patients with primary intracranial neoplasms

Abstract

Plasma from patients with malignant and benign primary intracranial neoplasms and from healthy control subjects has been examined using proton (1H) and carbon-13 nuclear magnetic resonance (NMR) spectroscopy. Some features in the spectra of plasma from patients with malignant tumors differ significantly (p less than 0.01) from the corresponding features in the spectra of plasma from control subjects and from patients with benign tumors. The NMR spectral parameters vary consistently with the Kernohan grade of astrocytoma, which may suggest that they give a measure of tumor growth kinetics. The observed spectral differences are shown to be due to elevated levels of monounsaturated and polyunsaturated fatty acid residues in the plasma of cancer patients. It is proposed that these lipid residues arise from cell membranes shed from cells in growing tumors. The ability to follow tumor growth kinetics directly may be of considerable importance in elucidating the effects of primary intracranial neoplasm therapy.

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

“…Similar results which show that ROC analysis is of little diagnostic value have also been obtained by others (Otvos et al, 1991). Two further reports (Peeling et al, 1988;Hofeler & Scheulen, 1989) credit in vitro NMR spectroscopy with a greater capacity to detect cancer but they still found a considerable overlap between the various patient groups (Peeling et al, 1988), and inconsistent results when the experiment was repeated (Hofeler & Scheulen, 1989). They did not determine the serum triglyceride levels (Peeling et al, 1988;Hofeler & Scheulen, 1989).…”

Proton magnetic resonance spectroscopy: clinical applications in patients with brain lesions

“…can be used to obtain magnetic resonance spectroscopy such as phosphorus, 5-7 carbon 8,9 and hydrogen. 10 The first in vivo magnetic resonance spectroscopy was performed at the beginning of the 1980s and was done using resonance of the phosphorus nucleus ( 31 P), thereby revealing the energy metabolism of the tissue cells studied.…”

A study on the differences between oral squamous cell carcinomas and normal oral mucosas measured by Fourier transform infrared spectroscopy

“…In recent years studies by optical and magnetic resonance spectroscopic methods in the field of bioscience and medicine, [1][2][3][4] have been progressing. Fourier-transform infrared spectroscopy (FTIR) is also becoming an increasingly powerful tool for the study of structure and function of cellular components in intact tissues.…”

“…Further, when teens (3 cases) in Table I were compared with above thirties (3 cases), there was no significant difference between them, and 3 cases of the elder group were inclined to shift to the opposite direction as compared with the change between normal and malignant tissues: for example, the average peak wavenumbers of the asymmetric PO 2 Ϫ and symmetric PO 2 Ϫ stretching bands in 3 cases of teens were 1246 cm Ϫ1 and 1084 cm Ϫ1 , respectively; however, those in 3 cases of the elder group were 1248 cm Ϫ1 and 1082 cm Ϫ1 . On the other hand, those in malignant tissues were 1242 cm Ϫ1 and 1086 cm Ϫ1 .…”

“…On the other hand, those in malignant tissues were 1242 cm Ϫ1 and 1086 cm Ϫ1 . Figure 4 exhibits the intensity ratio (R) of the asymmetric PO 2 Ϫ stretching band at around 1245 Second derivative infrared spectra computed from the spectra shown in Figure 2 are shown in Figure 5. Table II summarizes band positions (peak and trough in the second derivative) which are found in OSCC and NGE in the second derivative infrared spectra, with significant differences (pϽ 0.05).…”