1H and 11B NMR evidence for specific binding of borate ion to cytochrome c

Taler, Galia; Schejter, Abel

doi:10.1016/s0020-1693(97)06059-3

Cited by 9 publications

(16 citation statements)

References 17 publications

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“…The expressions for the R i D and the R i Q of the bound borate or boric acid are given in Eqs. [7] and [8], respectively. R i D and the R i Q of the boric acid or borate ion in solution where o c Ӷ 1 are…”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…In the presence of cytochrome c the appearance of new peaks was detected in the 11 B NMR spectrum (7). These peaks were assigned to borate ion specifically bound to the protein and exchanging slowly with the free borate in solution (7).…”

Section: Introductionmentioning

confidence: 99%

“…This interaction is known to affect the mobility of the electron carrier protein and has been interpreted as an extra role for the protein as an ion carrier (5,6). Recent 1 H and 11 B NMR measurements revealed that the tetrahedral borate anion, B(OH) 4 Ϫ , binds specifically to cytochrome c (7). Borate ion can serve as a model compound for studying the ion interaction with the cytochrome's surface and can be studied by both 1 H and 11 B NMR.…”

Section: Introductionmentioning

confidence: 99%

“…These peaks were assigned to borate ion specifically bound to the protein and exchanging slowly with the free borate in solution (7).…”

Section: Introductionmentioning

confidence: 99%

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Detection and Characterization of Boric Acid and Borate Ion Binding to Cytochrome c Using Multiple Quantum Filtered NMR

Taler

Eliav

1999

Journal of Magnetic Resonance

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Section: Theoretical Backgroundmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…These peaks were assigned to borate ion specifically bound to the protein and exchanging slowly with the free borate in solution (7).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection and Characterization of Boric Acid and Borate Ion Binding to Cytochrome c Using Multiple Quantum Filtered NMR

Taler

Eliav

1999

Journal of Magnetic Resonance

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“…10a shows the 11 B spectrum at 11.4 T of a 100 mM borate solution with 4 mM ferricytochrome-c at pH 9.7 and 5 • C. The large peak at 7.1 ppm (peak z) has the same shift as that of the weighted average of 70% B(OH) 4 − exchanging with 30% of B(OH) 3 . The peaks at 2.1 (peak y) and 1.8 (peak x) ppm are assigned to B(OH) 4 − specifically bound to two conformations of ferricytochrome-c that are present at pH 9.7 [76]. The assignment is based on the chemical shift which is similar to that of free B(OH) 4 − (2.0 ppm), and the narrow line-width of 14 Hz which is consistent with the tetrahedral symmetry of this species [76].…”

Section: Boronmentioning

confidence: 99%

Applications of heteronuclear NMR spectroscopy in biological and medicinal inorganic chemistry

Ronconi

Sadler

2008

Coordination Chemistry Reviews

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There is a wide range of potential applications of inorganic compounds, and metal coordination complexes in particular, in medicine but progress is hampered by a lack of methods to study their speciation. The biological activity of metal complexes is determined by the metal itself, its oxidation state, the types and number of coordinated ligands and their strength of binding, the geometry of the complex, redox potential and ligand exchange rates. For organic drugs a variety of readily observed spin I = 1/2 nuclei can be used (1H, 13C, 15N, 19F, 31P), but only a few metals fall into this category. Most are quadrupolar nuclei giving rise to broad lines with low detection sensitivity (for biological systems). However we show that, in some cases, heteronuclear NMR studies can provide new insights into the biological and medicinal chemistry of a range of elements and these data will stimulate further advances in this area

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Biomedical applications of10B and11B NMR

Bendel

2005

NMR Biomed.

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This review focuses mainly on the detection and investigation of molecules used for boron neutron capture therapy (BNCT) by 10 B and 11 B NMR. In this binary radiation treatment, boron-containing molecules (also called 'BNCT agents') enriched in the 10 B isotope, are targeted to the tumor, and irradiated with thermal or epithermal neutrons. Capture of these neutrons by 10 B nuclei generates cell-damaging radiation, confined to single cell dimensions. NMR research efforts have primarily been applied in two directions: first, to investigate the metabolism and pharmaco-kinetics of BNCT agents invivo, and second, to use localized NMR spectroscopy and/or MRI for non-invasive mapping of the administered molecules in treated animals or patients. While the first goal can be pursued using 11 B NMR for natural-abundance samples (80% 11 B / 20% 10 B), molecules used in the actual treatment are >95% enriched in 10 B, and must therefore be detected by 10 B NMR. Both 10 B (spin 3) and 11 B (spin 3/2) are quadrupolar nuclei, and their typical relaxation times, in common BNCT agents in biological environments, are rather short. This poses some technical challenges, particularly for MRI, which will be reviewed, along with possible solutions. The first attempts at 11 B NMR and MRI detection of BNCT agents in biological tissue were conducted over a decade ago. Since then, results from 11 B MRI in laboratory animals and in humans have been reported, and 11 B NMR spectroscopy provided interesting and unique information about the metabolism of some BNCT agents in cultured cells. 10 B NMR was applied either 'indirectly' (in double-resonance experiments involving coupled protons), but also by direct 10 B MRI in mice. However, no results involving the NMR detection of 10 B-enriched compounds in treated patients have been reported yet.

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1H and 11B NMR evidence for specific binding of borate ion to cytochrome c

Cited by 9 publications

References 17 publications

Detection and Characterization of Boric Acid and Borate Ion Binding to Cytochrome c Using Multiple Quantum Filtered NMR

Detection and Characterization of Boric Acid and Borate Ion Binding to Cytochrome c Using Multiple Quantum Filtered NMR

Applications of heteronuclear NMR spectroscopy in biological and medicinal inorganic chemistry

Biomedical applications of10B and11B NMR

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