1H, 13C, 15N chemical shift assignments for the Neisseria gonorrhoeae MinE regulator of cell division septum placement

Abstract

MinE acts together with MinC and MinD to prevent placement of the cell division septum in the polar regions of gram negative bacteria, thereby ensuring that productive cell division occurs solely at the mid-cell. Here we report the backbone and side chain (1)H, (13)C and (15)N resonance assignments for MinE from Neisseria gonorrhoeae.

Appropriation of the MinD protein-interaction motif by the dimeric interface of the bacterial cell division regulator MinE

“…Using standard heteronuclear solution NMR techniques, an ensemble of structures was determined for a full-length Ng-MinE sample. This was done using the E46A mutant, because it exhibited more favorable solubility characteristics than the wild-type protein, and it retained structural characteristics that were highly similar to that of WTas determined by circular dichroism and backbone NMR secondary chemical shifts (17,18). As shown in Fig.…”

“…NMR spectra were recorded at 298 K on either a 500-or an 800-MHz Varian Unity Inova spectrometer equipped with a cryoprobe. 1 H, 15 N, and 13 C resonance assignments were obtained for E46A Ng-MinE as described elsewhere (18). NMR samples were typically 0.7-1.0 mM of uniformly labeled 15 N-or 13 C, 15 N-labeled E46A Ng-MinE in a buffer containing 22.5 mM Tris-HCl at pH 7.2, 45 mM NaCl, 0.1 mM EDTA, 0.2 mM benzamidine, 0.02% NaN 3 , and 1 mM 2,2-dimethyl-2-silapentane-5-sulphonic acid.…”