1D∼3D Nano‐engineered Biomaterials for Biomedical Applications

Chen, Hui; Li, Xiaokang; Du, Yanan

doi:10.1002/9781118482513.ch1

Cited by 4 publications

(4 citation statements)

References 115 publications

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“…Surface modification of bacteria through both synthetic bioengineering and physicochemical technologies has been reviewed, and the application of modified bacteria as therapeutics and delivery vehicles for bioimaging, diagnosis, and therapy has been also introduced. 24 Differently, in this Account, we focus on surface coating of bacteria through physical (mechanical extrusion), chemical (interfacial selfassembly and mineralization), and biological (external stimulations) strategies, which endow coated bacteria with various characteristics of increased bioavailability, improved biosafety, and enhanced therapeutic efficacy for the applications in treating different diseases.…”

Section: Surface Coating Of Bacteriamentioning

confidence: 99%

Coated Bacteria: Advanced Living Materials for Microbial Therapy

Cao,

Liu

2024

Acc. Mater. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Living materials represent an emerging and innovative concept at the forefronts of materials science due to the distinctive living characteristics. Living organisms, such as microorganisms, plant cells, and mammalian cells, have been used as the major elements for the fabrication of living materials. Given the favorable features including reproductivity, genetic modifiability, adaptability, and cultivability, bacteria have received increasing attention for the development of engineered living materials. Particularly, owing to the inherent abilities to target and colonize specific in vivo sites, activate immune responses, and inhibit pathogens, bacteria play key roles in maintaining human health and have currently widely exploited as living materials for the preparation of therapeutic agents. However, caused by the innate immunogenicity, metabolic activity, and movability, the use of bacteria-based living materials for therapeutic applications often suffers from poor biosafety and insufficient bioavailability, inevitably resulting in low treatment efficacy. In order to address these challenges, extensive efforts have been invested in designing and engineering various advanced bacteria-based living materials to generate superior therapeutic agents by using genetic modification and surface decoration.In this Account, we summarize the recent advances from our group with a focus on surface coating of bacteria-based living material for advanced microbial therapy. First, we present a brief introduction of the existing living materials in nature that possess the living characteristics of reproduction, biosynthesis, self-healing, self-organization, self-adaption, and environment responsiveness, with a particular emphasis on the advantages of living materials composed by bacteria for microbial therapy. Second, we elaborate the current challenges of using bacteria-based living materials for therapeutic applications and then showcase the strategies to prepare superior living bacterial therapeutics by surface coating of individual bacteria. Three types of coating strategies are included in this Account: (1) the physical strategy to coat bacteria with eukaryotic cell membranes from red blood cells and yeast cells by mechanical extrusion;(2) the chemical strategy to wrap bacteria with synthetic coatings by interfacial self-assembly and interfacial mineralization;(3) the biological strategy to encapsulate bacteria by self-secreted biofilms and apoptosis bodies through external stimulations. We highlight that coated bacteria can be endowed with a wide range of coating-derived exogenous functions and enumerate their appealing characteristics of reduced immune clearance, increased bioavailability, improved safety, and preferential colonization in vivo. Third, we shift to the potential of these coated bacteria as living therapeutic agents for treating various diseases, especially different types of cancer and intestinal inflammations. Lastly, we discuss the remaining challenges that ...

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Section: Surface Coating Of Bacteriamentioning

confidence: 99%

Coated Bacteria: Advanced Living Materials for Microbial Therapy

Cao,

Liu

2024

Acc. Mater. Res.

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“…Synthesis of nanoscale structures of inert metals like gold is of great interest for the current day researchers as gold possess certain physical properties, which are suitable for several biomedical applications. Thus gold nanoparticles (AuNps) having unique physicochemical features show significant future promise in the fields of biomedical imaging and therapy [1][2][3][4][5]. Studies also lay special emphasis on the uniqueness of the gold nanoparticles for treatment of life threatening diseases like cancer [6].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast Comet Assay

Suvarna¹,

Nairy²,

Sunil³

et al. 2017

J Clinic Toxicol

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In the present study gold nanoparticles and glucose capped gold nanoparticles are synthesized by chemical route method and characterized using UV-SPR, FTIR and TEM analysis. Single cell gel electrophoresis (SCGE) assay was used to study DNA damage. Studies show that glucose capped gold nanoparticles are less toxic as compare to gold nanoparticles at DNA level. Somewhat larger gold nanoparticle used to monitor endocytosis in log-phase S. cervisiae spheroplasts at 10 to 30 µM was not reported to cause growth inhibition. It shows that glucose capped gold nanoparticles are nontoxic to yeast strain D7. DNA damage was observed by using standard method called Yeast comet assay, which provides a very sensitive method for detecting strand breaks and repair kinetics in single cells. Studies showed that 5 µM-30 µM having very less sign of DNA damage in case of Glucose capped gold nanoparticles and it also shows toxic effect for without glucose capped gold nanoparticles. OTM for different concentration as shown in the image and OTM with respect to different concentration shows the DNA damage, these studies also correlated with survival studies.

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“…cib and LMC could effectively improve angiogenesis and alleviate hypoxia, which are critical factors in promoting wound healing in DFUs. Considering the nonhealing pathological process of DFUs, the dual treatment of alternately delivering medical gases may have applications in other chronic diseases such as cardiac and hepatic ischemia injury. , It should be noted that the therapeutic mechanism of medicinal gases may be influenced by factors such as the cycle and concentration ratio of NO and O 2 , and thus, it requires careful evaluation. Further research will also be necessary to better understand the safety and tolerability of gaseous drugs.…”

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confidence: 99%

Two-Pronged Microbe Delivery of Nitric Oxide and Oxygen for Diabetic Wound Healing

Chen

et al. 2023

Nano Lett.

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Chronic inflammation and hypoxia in the microenvironment of diabetic foot ulcers (DFUs) can result in sustained vascular impairment, hindering tissue regeneration. While both nitric oxide and oxygen have been shown to promote wound healing in DFUs through anti-inflammatory and neovascularization, there is currently no available therapy that delivers both. We present a novel hydrogel consisting of Weissella and Chlorella, which alternates between nitric oxide and oxygen production to reduce chronic inflammation and hypoxia. Further experiments indicate that the hydrogel accelerates wound closure, re-epithelialization, and angiogenesis in diabetic mice and improves the survival of skin grafts. This dual-gas therapy holds promise as a potential treatment option for the management of diabetic wounds.

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1D∼3D Nano‐engineered Biomaterials for Biomedical Applications

Cited by 4 publications

References 115 publications

Coated Bacteria: Advanced Living Materials for Microbial Therapy

Coated Bacteria: Advanced Living Materials for Microbial Therapy

Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast Comet Assay

Two-Pronged Microbe Delivery of Nitric Oxide and Oxygen for Diabetic Wound Healing

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