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Abstract

The specie Guettarda platypoda DC. a shrub present in the flora of the state of Pernambuco (Itamaracá) is used in folk medicine as an antipyretic. The aim of this study was to conduct a bioactivity-guided fractionation of the leaves of this species, thus evaluating the biological activity (Cytotoxic and antioxidant) of substance and/or fractions of different polarities. The raw methanol extract of the leaves of G. platypoda was subjected to fractionation using solvents of increasing polarity; cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assay; antioxidant activity was determined based on the ability to sequester free radicals by the method of DPPH (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the molecular characterization was performed by spectrometric methods. From this work it was possible to isolate a porphyrin (pheophytin a). During bioguided tests, it was evident that the crude methanolic extract (IC50 = 7.02 x 10 -3 mg/mL), fractions of ethyl acetate (IC50 = 5.58 x 10 -3 mg/mL) and methanol (IC50 = 8.10 -3 mg/mL) had higher antioxidant activity than vitamin C. In conclusion, the substances present in the extract in ethyl acetate (F3) of G. platypoda and in the F2.2 subfraction, demonstrated cytotoxic and antioxidant activity against all cell lines tested, but the pheophytin a (P5), first identified to the genus Guetarda, showed no significant activity against the tested cell lines in this work.

Fitoquímicos E Atividade Antioxidante Das Espécies Lafoensia Pacari E Calycophyllum Spruceanum

“…Figueiredo et al (2009), estudando a espécie Richardia brasiliensis, pertencente à mesma família do C. spruceanum, seguiu a mesma metodologia deste estudo e encontraram na parte aérea das raízes: alcaloides, flavonoides, esteroides, triterpenoides, cumarinas e resina, enquanto nas raízes foram detectados esteroides, triterpenoides, cumarinas e resinas. Rodrigues et al (2014), analisando a espécie Guettarda platypoda, da mesma família citada anteriormente, obtiveram como resultado da triagem a presença de flavonoides, taninos, monoterpenoides, sesquiterpenoides e diterpenoides, triterpenoides, esteroides, saponinas e açúcares redutores. Porém, utilizaram a identificação por cromatografia em camada delgada, utilizando as fases móveis e reveladores específicos descritos na literatura para cada grupo de metabólitos.…”