193 nm Laser photoionization and photodissociation for isomer differentiation in Fourier-transform mass spectrometry

Williams, Evan R.; McLafferty, Fred W.

doi:10.1016/1044-0305(90)85015-e

Cited by 33 publications

(15 citation statements)

References 29 publications

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“…In multiple excitation collisional activation (MECA), 7,8 precursor ions that do not dissociate after the first excitation step are re-excited several times until dissociation occurs. Fragmentation efficiency is significantly improved using this method.…”

Section: Introductionmentioning

confidence: 99%

Internal Energy Distributions Resulting from Sustained Off-Resonance Excitation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. II. Fragmentation of the 1-Bromonaphthalene Radical Cation

2000

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The collision energy dependence of the fragmentation of the 1-bromonaphthalene radical cation was studied using sustained off-resonance excitation (SORI) in a 7 T Fourier transform ion cyclotron resonance mass spectrometer (FTMS). Fragmentation efficiency curves were obtained as a function of collision energy at four different pressures of Ar bath gas corresponding to collision numbers of 3, 5, 15, and 20. The results were modeled using RRKM/QET formalism. A refined analytical form for the collisional energy deposition function is proposed. The ability to obtain accurate fragmentation energetics of a complex system using the present approach is demonstrated. The "effective temperatures" deduced from the average internal energies for C 6 H 5 Br +• and C 10 H 7 Br +• were found to be the same for both ions provided the bath gas pressure and the maximum value of center-of-mass collision energy were the same. The range of effective temperatures from 1000 to 3700 K sampled in the present study significantly exceeds the temperature range accessible by blackbody infrared radiative dissociation (BIRD). We anticipate that the present approach can be used to study fragmentation energetics of biomolecules. IntroductionCollision-induced dissociation (CID) is a powerful tool both for determination of ion structures in the gas phase and for obtaining information on energetics and mechanisms of fragmentation processes of internally excited ions. Principles of collisional activation (CA) as well as various aspects and challenges of CA of polyatomic ions have been extensively discussed in several recent reviews. [1][2][3][4] The present study is a continuation of our group's ongoing research focused on characterizing the energy deposition function following sustained off-resonance irradiation CID (SORI-CID) in Fourier transform ion cyclotron resonance mass spectrometer (FTMS). Collisional activation in FTMS can be implemented using either on-resonance or off-resonance excitation of the precursor ion. In the former case, ions are accelerated to a desired kinetic energy with a short radio frequency (rf) pulse applied at the cyclotron frequency of the precursor ion 5,6 and activated by collisions with neutral atoms or molecules inside the ICR cell. This technique has been used to study fragmentation of relatively small ions under single-collision conditions. If multiple collisions occur, the kinetic energy of ions is effectively damped, and the efficiency of subsequent collisions is small. However, multiple collisions are clearly required to induce substantial fragmentation of large molecules (e.g., protonated peptides and proteins). Fragmentation efficiency of large molecules is limited by both (1) the decrease in the center-of-mass (CM) collision energy with increase in the mass of the precursor ion and (2) the dramatic decrease in decomposition rates with increase in the number of internal degrees of freedom.

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Section: Introductionmentioning

confidence: 99%

Internal Energy Distributions Resulting from Sustained Off-Resonance Excitation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. II. Fragmentation of the 1-Bromonaphthalene Radical Cation

2000

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“…Here, we demonstrate that high mass resolving power and ion-trapping capabilities of FT-ICR mass spectrometry can be utilized to differentiate GC eluted ␣-pinene and camphene isomers in real time. Other researchers reported isomer differentiation based on multiphoton ionization and dissociation (MPI/MPD) [33], GC/MPI-FTMS [34], FTMS proton affinity bracketing [35], collision-induced dissociation (CID) [36 -38], CID combined with chromatography [39], ion-molecule reactions combined with chromatographic techniques [40], oxygen negative chemical ionization (ONCI) [41], NCI GC [42], charge inversion MS [43], and by metal oxide sensors [44]. Modifying chromatographic parameters such as GC temperature programming or column type that can alter elution time and/or order will not change mass spectral appearances.…”

mentioning

confidence: 99%

A preconcentrator coupled to a GC/FTMS: Advantages of self-chemical ionization, mass measurement accuracy, and high mass resolving power for GC applications

Solouki

Szulejko

Bennett

et al. 2004

J. Am. Soc. Mass Spectrom.

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“…The first photoactivation at 193 nm on peptides goes back to the early 1980's (Bowers et al, ; Hunt et al, ; Lebrilla et al, ). In the 1990s, McLafferty has pioneered 193 nm photodissociation of electrosprayed protein ions (Williams & McLafferty, ). The Biemann group also implemented 193 nm photodissociation as a MS/MS technique on a four sectors instrument (Martin et al, ).…”

Section: Photoactivation Of Protonated Ionsmentioning

confidence: 99%

“…The appearance of lasers opened new opportunities for this field, as intense source with low divergence were within reach (Cotter, ). Countless applications have emerged on multiphoton ionization and photodissociation of electrosprayed and MALDI generated ions (Williams & McLafferty, ; Williams, Furlong, & McLafferty, ; Guan et al, ; Khoury, Rodriguez‐Cruz, & Parks, ; Reilly, ; Madsen, Boutz, & Brodbelt, ; Antoine & Dugourd, ). Although lasers are suitable photon sources for ion activation in terms of power and divergence, they are still limited to the visible (VIS) and ultraviolet (UV) photon energy range.…”

Section: Introductionmentioning

confidence: 99%

Contribution of synchrotron radiation to photoactivation studies of biomolecular ions in the gas phase

Giuliani

Milosavljević

Canon

et al. 2013

Mass Spectrometry Reviews

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Photon activation of ions in the visible and ultraviolet range attracts a growing interest, partly for its promising applications in tandem mass spectrometry. However, this task is not trivial, as it requires notably high brilliance photon sources. Hence, most of the work in that field has been performed using lasers. Synchrotron radiation is a source continuously tunable over a wide photon energy range and which possesses the necessary characteristics for ion activation. This review focuses on the array of applications of synchrotron radiation in photon activation of ions ranging from near UV to soft X-rays.

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193 nm Laser photoionization and photodissociation for isomer differentiation in Fourier-transform mass spectrometry

Cited by 33 publications

References 29 publications

Internal Energy Distributions Resulting from Sustained Off-Resonance Excitation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. II. Fragmentation of the 1-Bromonaphthalene Radical Cation

Internal Energy Distributions Resulting from Sustained Off-Resonance Excitation in Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. II. Fragmentation of the 1-Bromonaphthalene Radical Cation

A preconcentrator coupled to a GC/FTMS: Advantages of self-chemical ionization, mass measurement accuracy, and high mass resolving power for GC applications

Contribution of synchrotron radiation to photoactivation studies of biomolecular ions in the gas phase

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