190 Quantitative Autoradiographic Measurements of Blood Flow and Permeability in Experimental Rg-2 Rat Gliomas

Groothuis, Dennis R.; Fischer, Joan M.; Pasternak, Joseph F.; Bigner, Darell D.; Vick, Nicholas A.

doi:10.1097/00005072-198105000-00197

Cited by 17 publications

(27 citation statements)

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“…Comparing the present results with those of Blasberg and his colleagues (Blasberg et al, , 1985Groothuis et al, 1983) for the same method of measuring tumour blood flow provides some similarities between different tumour systems. They concluded, from a study on the RT-9 tumour growing in the subcutaneous flank tissue of CD Fisher rats, that tumour blood flow was consistently low in central tumour regions, usually below I ml 100 g-' min-' (Blasberg et al, 1985 Blasberg and colleagues also studied the RT-9 tumour and other transplanted tumour types growing in the brain Groothuis et al, 1983).…”

Section: Discussionsupporting

confidence: 84%

“…They concluded, from a study on the RT-9 tumour growing in the subcutaneous flank tissue of CD Fisher rats, that tumour blood flow was consistently low in central tumour regions, usually below I ml 100 g-' min-' (Blasberg et al, 1985 Blasberg and colleagues also studied the RT-9 tumour and other transplanted tumour types growing in the brain Groothuis et al, 1983). The mean blood flow to these tumours was much higher than that in the subcutaneous tumours.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, global measurements will include blood flow to necrotic tumour regions which are not relevant for therapy. For hyperthermia, a knowledge of high blood flow in discrete tumour regions would be important because these regions are the ones most likely to limit efficacy of treatment.Blasberg and colleagues used the inert, readily diffusible compound iodo-antipyrine to measure blood flow to transplanted rat brain tumours Groothuis et al, 1983). Labelling of iodo-antipyrine with 14C ('4C-IAP) facilitated autoradiography of tumour slices so that the regional distribution of tumour blood flow could be investigated.…”

mentioning

confidence: 99%

“…Blasberg and colleagues used the inert, readily diffusible compound iodo-antipyrine to measure blood flow to transplanted rat brain tumours Groothuis et al, 1983). Labelling of iodo-antipyrine with 14C facilitated autoradiography of tumour slices so that the regional distribution of tumour blood flow could be investigated.…”

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confidence: 99%

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The relationship between regional variations in blood flow and histology in a transplanted rat fibrosarcoma

Tozer

Lewis²,

Michałowski³

et al. 1990

Br J Cancer

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Summary The regional distribution of blood flow to the LBDS, fibrosarcoma, transplanted into the subcutaneous site in rats, was investigated using the readily diffusible compound '4C-iodo-antipyrine ('4C-IAP). Quantitative autoradiography was used to establish absolute values of specific blood flow F for 100 x 100 x 20 1sm adjacent tissue volumes of the unperturbed tumour. Mean blood flow to whole tumours was found to decrease with increase in tumour size. This relationship was abolished if blood flow was only measured in sections cut from the periphery of the tumours. Detailed analysis of a sub-group of tumours showed that blood flow to individual tumours was heterogeneous. The range of blood flow was large, indicating that mean blood flow to a whole tumour is a poor reflection of the blood perfusion pattern of that tumour. Necrotic tumour regions were usually very poorly perfused. With the exception of the smallest tumours studied, blood flow was lower in the centre of tumours than in the periphery. Necrosis also tended to develop centrally. However, the peripheral to central gradient of blood flow was apparent even when densely cellular, viable tumour regions and necrotic regions were analysed separately. The decrease in blood flow with tumour size was also apparent in densely cellular, viable tumour regions when analysed separately. Qualitative comparison of tumour histology and regional blood flow showed that there were areas of very low blood flow associated with viable tumour regions. Less common were areas of rather high blood flow associated with necrotic tumour regions. A complicated relationship exists between tumour histology and blood flow. The quantitative autoradiography technique is suitable for investigating the most poorly perfused and the most well perfused viable fractions of animal tumours which may limit the efficacy of different types of therapy.The blood flow to tumours plays an important role in their treatment. In radiotherapy, the efficacy of treatment depends upon cellular oxygen concentration which is governed by respiration rate and local blood flow. In chemotherapy, blood flow determines the efficiency of drug delivery. In hyperthermia, the temperature elevation achieved and the sensitivity of cells to heat is influenced by blood flow. Blood flow is therefore a critical parameter to measure both experimentally and clinically.It is known that blood flow to both transplanted animal tumours and to human tumours is heterogeneous (Chaplin et al., 1987;Ito et al., 1982;Vaupel & Frinak, 1980). Therefore global measurements may not be the most pertinent means of describing tumour blood flow. For instance, global measurements will include blood flow to necrotic tumour regions which are not relevant for therapy. For hyperthermia, a knowledge of high blood flow in discrete tumour regions would be important because these regions are the ones most likely to limit efficacy of treatment.Blasberg and colleagues used the inert, readily diffusible compound iodo-antipyrine to measure blood flow to tr...

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The relationship between regional variations in blood flow and histology in a transplanted rat fibrosarcoma

Tozer

Lewis²,

Michałowski³

et al. 1990

Br J Cancer

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“…Glioblastoma multiforme (GBM) is characterized by a rich vascular network (Hossman and Bloink 1981;Blasberg et al 1983;Groothuis et al 1983) and intratumoral necrosis (Raza et al 2002). Both HIF-1α and HIF-2α proteins are expressed in human glioblastomas (Jensen 2006;Li et al 2009), with HIF-1α expression being mostly concentrated to areas of necrosis and at the tumor margin (Zagzag et al 2000).…”

Section: Non-small Cell Lung Carcinoma Hif Protein Expression Is Virmentioning

confidence: 99%

The HIF-2α-Driven Pseudo-Hypoxic Phenotype in Tumor Aggressiveness, Differentiation, and Vascularization

Pietras

Johnsson

Påhlman

2010

Current Topics in Microbiology and Immunology

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Rat Brain Tumor Models and the Statistical Evaluation of Survival Data in Experimental Neuro-Oncology

Barth¹,

Moeschberger²

1998

Gene Therapy for Neurological Disorders and Brain Tumors

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190 Quantitative Autoradiographic Measurements of Blood Flow and Permeability in Experimental Rg-2 Rat Gliomas

Cited by 17 publications

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The relationship between regional variations in blood flow and histology in a transplanted rat fibrosarcoma

The relationship between regional variations in blood flow and histology in a transplanted rat fibrosarcoma

The HIF-2α-Driven Pseudo-Hypoxic Phenotype in Tumor Aggressiveness, Differentiation, and Vascularization

Rat Brain Tumor Models and the Statistical Evaluation of Survival Data in Experimental Neuro-Oncology

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