[19] The synthesis and use of fluorescent oligonucleotides in DNA sequence analysis

Smith, Lloyd M.; Kaiser, Robert; Sanders, Jane Z.; Hood, Leroy

doi:10.1016/0076-6879(87)55021-2

Cited by 54 publications

(25 citation statements)

References 7 publications

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“…A fully automated multicapillary electrophoresis device 1503 direction (no binning in the y-direction), the signals from both ªchannelsº of each capillary were baseline-subtracted and transformed by a 2´2 matrix for color deconvolution (analogous to the 4´4 matrices used in DNA sequencing [73]). The data from eight capillaries out of 96 are presented in Fig.…”

Section: Two-color Separationmentioning

confidence: 99%

A fully automated multicapillary electrophoresis device for DNA analysis

et al. 1999

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Section: Two-color Separationmentioning

confidence: 99%

A fully automated multicapillary electrophoresis device for DNA analysis

et al. 1999

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“…For conventional DNA sequencing, transformation of ''raw,'' multicomponent, fluorescent signals into ''processed,'' four-color sequence data involves a number of algorithmic manipulations such as removal of cross-talk, baseline adjustment, mobility dye correction, emission signal intensity normalization, and base-calling. Of these, determination of the cross-talk matrix, which allows for the conversion of mixed-fluorescent signals into concentration estimates for the dye-labeled DNA fragments at a given time point during a sequencing experiment, is likely the most relevant operation (21). Numerous research groups have described methods to determine a representative cross-talk matrix for DNA sequencing data, albeit with limited success (22)(23)(24)(25)(26)(27)(28).…”

Section: Pme Technologymentioning

confidence: 99%

Color-blind fluorescence detection for four-color DNA sequencing

Lewis

Haaland

Nguyen

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorption maxima span virtually the entire visible spectrum. Unlike emission wavelength-dependent approaches for identifying fluorescent species, the removal of the spectral component in PME confers a number of advantages including higher and normalized signals from all dyes present in the assay, the elimination of spectral cross-talk between dyes, and higher signal collection efficiency. Base-calling is unambiguously determined once dye mobility corrections are made. These advantages translate into significantly enhanced signal quality as illustrated in the primary DNA sequencing data and provide a means for achieving accurate base-calling at lower reagent concentrations.

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“…The "raw" fluorescence signals, however, must be transformed. Removal of cross-talk, correction for dye mobility alterations, and normalization of emission intensities must be performed before readable DNA sequence information can be obtained (Smith et al 1987). Base-calling and error probability assignment applications are then used to call the DNA sequence and assess the accuracy of the call.…”

Section: Sanger Sequencing: State-of-the-art Technologymentioning

confidence: 99%

Emerging technologies in DNA sequencing

Metzker¹

2005

Genome Res.

410

262

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Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of delivering next-generation solutions for fast and affordable genome sequencing. In this review article, the DNA polymerase-dependent strategies of Sanger sequencing, single nucleotide addition, and cyclic reversible termination are discussed to highlight recent advances and potential challenges these technologies face in their development for ultrafast DNA sequencing.

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[19] The synthesis and use of fluorescent oligonucleotides in DNA sequence analysis

Cited by 54 publications

References 7 publications

A fully automated multicapillary electrophoresis device for DNA analysis

A fully automated multicapillary electrophoresis device for DNA analysis

Color-blind fluorescence detection for four-color DNA sequencing

Emerging technologies in DNA sequencing

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