19 A Song (‘Myra was gentle, fair, and young’)

Crabbe, George; Dalrymple-Champneys, Norma; Pollard, Arthur

doi:10.1093/oseo/instance.00039921

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Sds-page Western Blotting and Confocal Microscopy1

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1998

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“…Paired L-sheets such as those seen in the Greek key/Immunoglobulin fold are employed in a wide variety of proteins to facilitate protein-protein interactions [12,13]. The simplest and earliest members of immunoglobulin family functioned as simple cellular adhesives [14].…”

Section: Sds-page Western Blotting and Confocal Microscopymentioning

confidence: 99%

Translocation of β crystallin in neural cells in response to stress

Coop¹,

Wiesmann²,

Crabbe³

1998

FEBS Letters

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While extralenticular expression of proteins in the K K crystallin (small heat shock protein) family is well documented, that for proteins in the L L/Q Q-superfamily is less well established. Here we show, using SDS-PAGE, Western blotting and confocal microscopy, that there is a constitutive level of L L crystallin expression in mouse N1E-115 neural cells. Furthermore, upon heat shock at 43³C or 55³C, or cold shock at 30³C, L L crystallin immunoreactivity translocated predominantly from the nuclear region into the cytoplasmic region of the cells. In conditions of stress, it may be important for L L crystallin to be recruited into the cytoplasm to stabilise other proteins via its high L L-sheet content, and/or to ensure that storage levels of cytoplasmic Ca P+ are maintained.z 1998 Federation of European Biochemical Societies.

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Section: Sds-page Western Blotting and Confocal Microscopymentioning

confidence: 99%