18S ribosomal DNA-based PCR test for avian and mammalian DNA identification in meat products

Abstract

Highlights 18S rRNA phylogenetic analysis of four main classes of Tetrapod is base of PCR test. The result of the test is different PCR fragments for avian and mammalian DNA. 18S rDNA based PCR test determines avian and mammalian DNA. 18S rDNA based PCR test determines avian and mammalian meat in different products.

Nuclear DNA in chicken egg whites?

“…To prove that the isolated DNA is of chicken origin, the PCR test based on the 18S rRNA gene has been used in this study 17 (Fig. 2).…”

“…DNA Analyzes. Isolated DNA samples have been checked for presence of avian DNA by the PCR test based on 18S ribosomal RNA (rRNA) gene17 (Fig.2). Speci c PCR fragments of 97 bp have been cloned and sequenced for LPAR, PUSH, and RHIR breeds (Gen Bank accession ON005571).…”

“…DNA Analyzes. 18S ribosomal DNA based PCR test for avian and mammalian DNA identi cation has been used17 . Ampli cation from primers of this test has been performed on the BIOER thermocycler (Hangzhou Bioer Technology Co, Ltd., China) using Phusion Hot Start II High-Fidelity DNA-polymerase (Thermo Scienti c).…”

Chicken nuclear DNA in chicken egg whites

“…18S ribosomal DNA based PCR test for avian and mammalian DNA identi cation was used (Zyrianova and Zaripov, 2022). Ampli cation from primers of this test was performed on the BIOER thermocycler (Hangzhou Bioer Technology Co, Ltd., China) using Phusion Hot Start II High-Fidelity DNA-polymerase (Thermo Scienti c).…”

“…Isolated DNA samples were checked for presence of avian DNA by the PCR test based on 18S rRNA gene (Zyrianova and Zaripov, 2022) (Fig. 2).…”