18O-incorporated De-N-glycosylated Site Identification: A Comparison of Three Search Engines

Abstract

Enzymatic de-N-glycosylation has become an essential procedure in most large scale studies to identify N-glycosylation sites because the removal of heterogeneous sugar moieties enables the detection of deglycosylated peptides by standard bottom-up proteomic pipelines. To differentiate deglycosylation from chemical deamidation, this reaction is often carried out in an H 2