183: Interleukin 1 beta is increased in the hippocampus and posterior cortex of rats with hypertension and systemic inflammation during pregnancy

Abstract

isolated proteinuria (n¼13, GA: 28AE6 wks). Misfoldome proteins were enriched by Congo Red precipitation and subjected to UPLC/MS/MS. Deep RNA sequencing (RNA-seq) was performed on decidua basalis (n¼5) and villous trophoblast (n¼5) of women with preterm severe PE (GA: 32AE1 wks). These reads, together with RNA-Seq data from the Illumina Human BodyMap 2.0 database (n¼16, adult tissues including kidney), were used to intersect specific transcripts that may contribute to aberrant proteinuria in PE. RESULTS: 646 unique proteins IDs comprised the urinary misfoldome of PE women. Our intersection analysis showed a potential contribution of villous trophoblast, decidua and kidney to the misfoldome (Figure). Villous trophoblast was a larger contributor than either kidney or decidua (Table). Kidney-specific transcripts such as solute carrier family 22 (SLC22A12, a urate transporter), SMIM24 (an uncharacterized small integral membrane protein), MMP7 (matrilysin), and CALB1 (calbindin 1, a protein linked to Huntington disease) mapped to distinct PE subphenotypes. CONCLUSION: The urinary misfoldome harbors a considerable number of peptides of placental and kidney origin which may interact to play key pathogenic roles in various PE subphenotypes. 183 Interleukin 1 beta is increased in the hippocampus and posterior cortex of rats with hypertension and systemic inflammation during pregnancy