18 Mammalian Alkaline Phosphatases

Alkaline phosphatase activity associated to a calcium binding glycoprotein from calf scapula cartilage

“…1. As for other alkaline phosphatases [9,10], the optimum pH is in the high alkaline regions. The maximal activity was found around pH 10, both in glyeine-NaOH and carbonate-bicarbonate buffer (not shown).…”

“…One of the cardinal features of alkaline phosphatases is their activation by divalent metal cations [10]. As illustrated in fig.…”

“…3. Another characteristic of alkaline phosphatases is the sensitivity to some inhibitors [10]. Assay conditions as in fig.…”

“…This enzyme was considered by Robison [8], to play an essential role in calcification. Since Robison's time many alkaline phosphatases have been extracted and purified (for a review see [9,10]) and for many of them a glycoprotein nature has been established [ 10]. It appears logical, therefore, our interest in studying the catalytic function of our glycoprotein preparation, alkaline phosphatase activity 'in primis'.…”

Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue

“…Assay was carried out using the method described earlier [17,18]. Briefly, the enzyme reaction was initiated by allowing 100 ll of the supernatant obtained following tissue homogenization to react with 900 ll of 5 mM pNPP (SIGMA) in 0.1 M-citrate buffer (pH 5.0) and incubated for 15 min at 37°C in a water bath.…”