18 F-choline in experimental soft tissue infection assessed with autoradiography and high-resolution PET

Wyss, Matthias T.; Weber, Bruno; Honer, Michael; Späth, Nicolas; Ametamey, Simon M.; Westera, G.; Bode, Beata; Kaim, Achim H.; Buck, Alfred

doi:10.1007/s00259-003-1337-4

Cited by 86 publications

(51 citation statements)

References 7 publications

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“…In addition to cancer imaging, choline-derived tracers have shown potential for inflammation imaging and accumulation in inflammatory cells (5,13). The metabolic activity and the production of nitric oxide may explain the choline uptake in macrophages (14), but this requires further study.…”

Section: Discussionmentioning

confidence: 99%

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Uptake of ¹¹C-Choline in Mouse Atherosclerotic Plaques

Laitinen¹,

Luoto²,

Någren³

et al. 2010

J Nucl Med

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The purpose of this study was to explore the feasibility of 11 C-choline in the assessment of the degree of inflammation in atherosclerotic plaques. Methods: Uptake of 11 C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR 2/2 ApoB 100/100 ) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites. Results: The uptake of 11 C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR 2/2 ApoB 100/100 mice was significantly higher (1.9-fold, P 5 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of 11 C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 6 0.6; P 5 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas. Conclusion: We observed a high 11 C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of 11 C-choline in the plaques.

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Section: Discussionmentioning

confidence: 99%

“…Phosphorylated by choline kinase, choline eventually incorporates into cell membranes. Increased choline transport and choline kinase activity in tumor cells and macrophages have been suggested to result in increased choline uptake in these cells (5,6).…”

mentioning

confidence: 99%

Uptake of ¹¹C-Choline in Mouse Atherosclerotic Plaques

Laitinen¹,

Luoto²,

Någren³

et al. 2010

J Nucl Med

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“…16 Hence, high membrane turnover in proliferating and in active cells is closely associated with an increased choline metabolism. [17][18][19] Radiolabeledcholine tracers are already used in clinical PET imaging of different cancers 17 and are under evaluation for imaging of different inflammatory conditions. 18,19 We showed enhanced 18 F-FCH uptake in human carotid plaques early after onset of a cerebrovascular event compared with a low uptake in asymptomatic plaques.…”

Section: Discussionmentioning

confidence: 99%

“…Carotid plaque was identified on contrast-enhanced CT images, defined as thickening of the vessel by guest on May 12, 2018 http://circimaging.ahajournals.org/ Downloaded from wall and calcification, and carotid stenosis grade was assessed using the North American Symptomatic Carotid Endarterectomy Trial method.…”

Section: F-fch Pet-ct Evaluationmentioning

confidence: 99%

“…16 Radiolabeled-choline PET tracers (including 18 F-FCH) are highly taken up by tumor cells 17 and also by active and proliferating macrophages involved in inflammatory processes. [18][19][20][21] A retrospective trial of tumor patients also assessing carotid atherosclerosis has shown a significantly increased choline uptake in noncalcified regions, assumed to be inflamed plaques, in comparison to the healthy vessel wall. 22 Interestingly, in a side-by-side comparison between 18 F-FCH and 18 F-FDG in murine atherosclerotic lesions, choline uptake correlated with macrophage staining even better than FDG uptake.…”

Section: Vöö Et Al 18f-fch Pet In Investigating Vulnerable Plaquesmentioning

confidence: 99%

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Imaging Intraplaque Inflammation in Carotid Atherosclerosis With ¹⁸ F-Fluorocholine Positron Emission Tomography–Computed Tomography

Vöö

Kwee

Sluimer

et al. 2016

Circ: Cardiovascular Imaging

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Background— 18 F-fluorocholine ( 18 F-FCH) uptake is associated with cell proliferation and activity in tumor patients. We hypothesized that 18 F-FCH could similarly be a valuable imaging tool to identify vulnerable plaques and associated intraplaque inflammation and atheroma cell proliferation. Methods and Results— Ten consecutive stroke patients (90% men, median age 66.5 years, range, 59.4–69.7) with ipsilateral >70% carotid artery stenosis and who underwent carotid endarterectomy were included in the study. Before carotid endarterectomy, all patients underwent positron emission tomography to assess maximum 18 F-FCH uptake in ipsilateral symptomatic carotid plaques and contralateral asymptomatic carotid arteries, which was corrected for background activity, resulting in a maximum target-to-background ratio (TBRmax). Macrophage content was assessed in all carotid endarterectomy specimens as a percentage of CD68 + -staining per whole plaque area (plaqueCD68 + ) and as a maximum CD68 + percentage (maxCD68 + ) in the most inflamed section/plaque. Dynamic positron emission tomography imaging demonstrated that an interval of 10 minutes between 18 F-FCH injection and positron emission tomography acquisition is appropriate for carotid plaque imaging. TBRmax in ipsilateral symptomatic carotid plaques correlated significantly with plaqueCD68 + (Spearman’s ρ=0.648, P =0.043) and maxCD68 + (ρ=0.721, P =0.019) in the 10 corresponding carotid endarterectomy specimens. TBRmax was significantly higher ( P =0.047) in ipsilateral symptomatic carotid plaques (median: 2.0; interquartile range [Q1–Q3], 1.5–2.5) compared with the contralateral asymptomatic carotid arteries (median: 1.4; Q1–Q3, 1.3–1.6). TBRmax was not significantly correlated to carotid artery stenosis (ρ=0.506, P =0.135). Conclusions— In vivo uptake of 18 F-FCH in human carotid atherosclerotic plaques correlated strongly with degree of macrophage infiltration and recent symptoms, thus 18 F-FCH positron emission tomography is a promising tool for the evaluation of vulnerable plaques. Clinical Trial Registration— URL: http://www.clinicaltrials.gov . Unique identifier: NCT01899014.

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