17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells

Wright, Peter V.; Jones, Sarah Bowen; Ardern, Nicholas; Ward, Rebecca; Clarke, Robert B.; Sotgia, Federica; Lisanti, Michael P.; Landberg, Göran; Lamb, Rebecca

doi:10.18632/oncotarget.1824

Cited by 32 publications

(25 citation statements)

References 34 publications

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“…Similar structures were detected in paraffin sections of tumors from mice with metastatic disease by staining with anti-ARF6 antibodies[13]. Large oncosomes resemble “giant vesicles” recently identified by an independent group in breast cancer lines and detected in tissue sections of human breast cancer[110]. The demonstrated correlation between the oncosome-like feature and tumor progression in tissues, albeit potentially significant, requires further investigation.…”

Section: Large Oncosomessupporting

confidence: 52%

Extracellular Vesicles in Cancer: Exosomes, Microvesicles and the Emerging Role of Large Oncosomes

Minciacchi

Freeman

Vizio

2015

Seminars in Cell & Developmental Biology

747

638

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Since their first description, extracellular vesicles (EVs) have been the topic of avid study in a variety of physiologic contexts and are now thought to play an important role in cancer. The state of knowledge on biogenesis, molecular content and horizontal communication of diverse types of cancer EVs has expanded considerably in recent years. As a consequence, a plethora of information about EV composition and molecular pathways involved in the regulation of important biological processes has emerged, along with the notion that cancer cells rely on these particles to invade tissues and propagate oncogenic signals at distance. In vivo studies, designed to achieve a deeper understanding of the extent to which EV biology can be applied to clinically relevant settings, are increasing. This review will summarize recent studies on EVs functionally implicated in cancer, with a focus on a novel EV population referred to as large oncosomes, which originate from highly migratory, amoeboid tumor cells. Here we provide an overview about the biogenesis and composition of exosomes, microvesicles and large oncosomes, along with their cancer-specific and more general functions. We also discuss current challenges and emerging technologies that might improve EV detection in various systems. Further studies on the functional role of EVs in specific steps of cancer formation and progression will expand our understanding of the diversity of paracrine signaling mechanisms in malignant growth.

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Section: Large Oncosomessupporting

confidence: 52%

Extracellular Vesicles in Cancer: Exosomes, Microvesicles and the Emerging Role of Large Oncosomes

Minciacchi

Freeman

Vizio

2015

Seminars in Cell & Developmental Biology

747

638

View full text Add to dashboard Cite

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“…EVs in the size range and appearance of LO have been recently described with different names including: (1) giant vesicles, identified in ERα-positive breast cancer cells and tumor tissues [ 49 ]; (2) migrasomes, large round structures containing numerous vesicles (pomegranate-like structures), which depart from retraction fibers of migratory benign cells [ 50 ]; and (3) tumor-derived MV originating from amoeboid-like tumor cells, in which VAMP3 seems to regulate the delivery of MV cargo to regions of high plasma membrane blebbing. MV appear to be released through blebbing during migration [ 51 ].…”

Section: The Variegated World Of Extracellular Vesiclesmentioning

confidence: 99%

Focus on Extracellular Vesicles: New Frontiers of Cell-to-Cell Communication in Cancer

Ciardiello

Cavallini

Spinelli

et al. 2016

IJMS

273

221

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Extracellular Vesicles (EVs) have received considerable attention in recent years, both as mediators of intercellular communication pathways that lead to tumor progression, and as potential sources for discovery of novel cancer biomarkers. For many years, research on EVs has mainly investigated either the mechanism of biogenesis and cargo selection and incorporation, or the methods of EV isolation from available body fluids for biomarker discovery. Recent studies have highlighted the existence of different populations of cancer-derived EVs, with distinct molecular cargo, thus pointing to the possibility that the various EV populations might play diverse roles in cancer and that this does not happen randomly. However, data attributing cancer specific intercellular functions to given populations of EVs are still limited. A deeper functional, biochemical and molecular characterization of the various EV classes might identify more selective clinical markers, and significantly advance our knowledge of the pathogenesis and disease progression of many cancer types.

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“… 23 They do not appear to be produced by benign cells. 39 More recently, the identification of similarly large EVs has been reported 21 , 40 , 41 but it is unclear whether they share similar functional or molecular characteristics to the LO.…”

Section: Evs Are Heterogeneousmentioning

confidence: 99%

Extracellular vesicles for liquid biopsy in prostate cancer: where are we and where are we headed?

Minciacchi

Zijlstra

Rubin

et al. 2017

Prostate Cancer Prostatic Dis

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Background:Extracellular vesicles (EVs) are a heterogeneous class of lipid bound particles shed by any cell in the body in physiological and pathological conditions. EVs play critical functions in intercellular communication. EVs can actively travel in intercellular matrices and eventually reach the circulation. They can also be released directly in biological fluids where they appear to be stable. Because the molecular content of EVs reflects the composition of the cell of origin, they have recently emerged as a promising source of biomarkers in a number of diseases. EV analysis is particularly attractive in cancer patients that frequently present with increased numbers of circulating EVs.Methods:We sought to review the current literature on the molecular profile of prostate cancer-derived EVs in model systems and patient biological fluids in an attempt to draw some practical and universal conclusions on the use of EVs as a tool for liquid biopsy in clinical specimens.Results:We discuss advantages and limitations of EV-based liquid biopsy approaches summarizing salient studies on protein, DNA and RNA. Several candidate biomarkers have been identified so far but these results are difficult to apply to the clinic. However, the field is rapidly moving toward the implementation of novel tools to isolate cancer-specific EVs that are free of benign EVs and extra-vesicular contaminants. This can be achieved by identifying markers that are exquisitely present in tumor cell-derived EVs. An important contribution might also derive from a better understanding of EV types that may play specific functions in tumor progression and that may be a source of cancer-specific markers.Conclusions:EV analysis holds strong promises for the development of non-invasive biomarkers in patients with prostate cancer. Implementation of modern methods for EV isolation and characterization will enable to interrogate circulating EVs in vivo.

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17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells

Cited by 32 publications

References 34 publications

Extracellular Vesicles in Cancer: Exosomes, Microvesicles and the Emerging Role of Large Oncosomes

Extracellular Vesicles in Cancer: Exosomes, Microvesicles and the Emerging Role of Large Oncosomes

Focus on Extracellular Vesicles: New Frontiers of Cell-to-Cell Communication in Cancer

Extracellular vesicles for liquid biopsy in prostate cancer: where are we and where are we headed?

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