17-β Estradiol Protects ARPE-19 Cells from Oxidative Stress through Estrogen Receptor-β

Giddabasappa, Anand; Bauler, Matthew; Yepuru, Muralimohan; Chaum, Edward; Dalton, James T.; Eswaraka, Jeetendra

doi:10.1167/iovs.10-5316

Cited by 46 publications

(62 citation statements)

References 34 publications

(29 reference statements)

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“…According to these data, E2:ERb complex-mediated cytoprotection from oxidative stress has been reported also in ARPE cells (Giddabasappa et al 2010). As a consequence, our data strongly support that Nar-mediated skeletal muscle protection from ROS-induced oxidative stress depends on its ERb agonist activity rather than a free radical quenching compound activity.…”

Section: Discussionsupporting

confidence: 86%

Naringenin modulates skeletal muscle differentiation via estrogen receptor α and β signal pathway regulation

et al. 2014

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Several experiments sustain healthful benefits of the flavanone naringenin (Nar) against chronic diseases including its protective effects against estrogen-related cancers. These experiments encourage Nar use in replacing estrogen treatment in post-menopausal women avoiding the serious side effects ascribed to this hormone. However, at the present, scarce data are available on the impact of Nar on E2-regulated cell functions. This study was aimed at determining the impact of Nar on the estrogen receptor (ERa and b)-dependent signals important for 17b-estradiol (E2) effect in muscle cells (rat L6 myoblasts, mouse C2C12 myoblasts, and mouse skeletal muscle satellite cells). Dietary relevant concentration of Nar delays the appearance of skeletal muscle differentiation markers (i.e., GLUT4 translocation, myogenin, and both fetal and slow MHC isoforms) and impairs E2 effects specifically hampering ERa ability to activate AKT. Intriguingly, Nar effects are specific for E2-initiating signals because IGF-Iinduced AKT activation, and myoblast differentiation markers were not affected by Nar treatment. Only 7 days after Nar stimulation, early myoblast differentiation markers (i.e., myogenin, and fetal MHC) start to be accumulated in myoblasts. On the other hand, Nar stimulation activates, via ERb, the phosphorylation of p38/MAPK involved in reducing the reactive oxygen species formation in skeletal muscle cells. As a whole, data reported here strongly sustain that although Nar action mechanisms include the impairment of ERa signals which drive muscle cells to differentiation, the effects triggered by Nar in the presence of ERb could balance this negative effect avoiding the toxic effects produced by oxidative stress.

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Section: Discussionsupporting

confidence: 86%

Naringenin modulates skeletal muscle differentiation via estrogen receptor α and β signal pathway regulation

et al. 2014

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“…Both ERa and ERb have been identified in human retina. 18,19 In fact, Ogueta and colleagues in 1999 found that ERa concentrations decreased with age (35 > 49 > 74 years) in women, whereas males had ERa levels that were between those of 49-and 74-year-old women. 20 In addition, some studies do report a difference in the occurrence of PDR, which can be attributed to puberty.…”

mentioning

confidence: 99%

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

Grigsby

Parvathaneni

Almanza

et al. 2011

Journal of Ocular Pharmacology and Therapeutics

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Purpose: Endothelial cell proliferation in angiogenesis is active in conditions such as cancers and diabetic retinopathy. Tamoxifen (T) and raloxifene (R) have been compared in numerous studies as a prophylaxis for breast cancer, and T is used to treat breast cancer. T, unlike R, has been linked to an increase in uterine cancers, thrombo-embolic events, and cataract. The purpose of our study was to evaluate the efficacies of T and R in reducing estrogen-induced retinal capillary endothelial cell proliferation. Methods: Rhesus monkey retinal capillary endothelial cells (ATCC RF/6A) were used to assay cell proliferation when treated with 0.0, 0.1, 1.0, and 10.0 nM 17 b estradiol (E2) for 24 and 48 h. Viable cells were counted using a Neubauer hemocytometer with a trypan blue exclusion method to determine the number of viable cells. Cell counts were also performed using 1.0 nM E2 with 0.01, 0.1, 1.0, and 10.0 nM concentrations of either T or R. Cell medium, collected at 24 h, was evaluated for vascular endothelial growth factor and pigment epithelium-derived factor. Results: Viable cells were significantly greater in cultures treated with 1.0 or 10.0 nM E2, compared to cells treated with 0.0 or 0.1 nM E2 both at 24 and 48 h. Viable cell counts were reduced significantly in cultures treated with 0.1, 1.0, or 10.0 nM T or R in addition to the 1.0 nM E2. Cell counts were not significantly different when comparing equal concentrations of T and R, that is, 1.0 nM E2 + 1 nM T or R. Vascular endothelial growth factor and pigment epithelium-derived factor protein/10,000 cells was reduced by 1.0 nM E2, but returned to higher levels with the introduction of T and R to growth media. Conclusions: T and R showed similar potency in inhibiting estrogen-induced retinal capillary endothelial cell proliferation. Considering drug safety profiles, our results, when extended to animals and humans, suggest that R is preferable to T in treating angiogenic retinal diseases. Further studies on the signaling mechanism of estrogen-induced endothelial cell proliferation may lead to new treatment strategies in the treatment of ocular angiogenic diseases.

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“…Estrogen receptors have been found in the human RPE, and estrogen provides an antioxidant effect by inhibiting lipid peroxidation. Related studies revealed that patients exposed to estrogen due to hormone replacement therapy or pregnancy had increased incidence of AMD (18)(19)(20)(21)(22). Pathogenesis of AMD is multifactorial and choroidal thickness might be playing a role in this broad pathogenetic cycle of the disease.…”

Section: Discussionmentioning

confidence: 99%

Choroidal thickness in healthy Turkish subjects

Coşkun¹,

Okumuş²,

Gürler³

et al. 2014

Turk J Med Sci

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Flow injection cold vapor atomic absorption spectrometry (FI-CVAAS) was used for determination of cadmium in high salt matrices such as dialysis concentrates and textile leach solutions. The parameters such as acidity, concentration of reducing agent, reaction coil length, and carrier gas flow rate were investigated to obtain the best sensitivity. No significant background signal was observed even at high salt concentrations. Under the optimized conditions the limit of detection value (3s b m −1 , where sb is the standard deviation of the blank signals and m is the slope of the calibration graph) was found to be 0.05 ng mL −1 for an injection volume of 250 μ L and the precision in terms of relative standard deviation was 3.2% using 11 consecutive measurements of 0.5 ng mL −1 standard. The standard addition method was used for quantitation. The accuracy of the method was tested using ETAAS as a comparison method and a certified reference material (Waste Water EU-L-1). In both cases, the results were in good agreement at 95% confidence level. The concentrations of cadmium in textile product leach solutions were found to be below the limit value assigned by Oeko-Tex standards.

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17-β Estradiol Protects ARPE-19 Cells from Oxidative Stress through Estrogen Receptor-β

Abstract: These results indicate that 17β-E(2) protects ARPE-19 cells from oxidative stress through an ERβ-dependent mechanism. 17β-E(2)-mediated cytoprotection occurred through the preservation of mitochondrial function, reduction of ROS production, and induction of cellular antioxidant genes.

Cited by 46 publications

References 34 publications

Naringenin modulates skeletal muscle differentiation via estrogen receptor α and β signal pathway regulation

Naringenin modulates skeletal muscle differentiation via estrogen receptor α and β signal pathway regulation

Effects of Tamoxifen Versus Raloxifene on Retinal Capillary Endothelial Cell Proliferation

Choroidal thickness in healthy Turkish subjects

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