17<b>β</b>-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary

Stępniak, Jan; Karbownik-Lewińska, Małgorzata

doi:10.3109/19396368.2015.1101510

Cited by 33 publications

(31 citation statements)

References 37 publications

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“…The biomarker, 8-OHdG, released secondary to DNA damage, is regarded as the most significant indicator of DNA damage. 52,53) Hydroxyl radicals eliminate hydrogen from nucleic acids or react with double bonds, leading to 8-OHdG. 54) There have been various studies suggesting that DNA damage decreases by anti-oxidant augmentation, 55,56) and our results in the current study support these findings, wherein naringenin utilized its anti-oxidant properties to decrease ROS-mediated 8-OHdG levels indicating the inhibition of oxidative DNA damage.…”

Section: Discussionsupporting

confidence: 78%

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

Ganaie

Jan

Khan

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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Oxaliplatin is a third generation platinum based anti-cancer drug used against various human malignancies but displays genotoxic properties against normal cells. Naringenin is a naturally occurring bioflavonoid that possesses anti-oxidant properties and has protective effects against DNA damage. The aim of this study is to examine the protective effects of naringenin on oxaliplatin-induced DNA damage in mice. A total of 50, male BALB/c mice were randomly divided equally into five groups. Oxaliplatin toxicity was induced by a single dose (7 mg/kg body weight (b.w.)) injection (intraperitoneally (i.p.)) of oxaliplatin. Naringenin was given orally for ten consecutive days at two doses, 20 mg/kg b.w. (dose I) and 40 mg/kg b.w. (dose II), to group I and group II, respectively. On the tenth day of the experiment, animals in groups III, IV, and V were given a single i.p. injection of oxaliplatin (7 mg/kg b.w.). All the animals were sacrificed 24 h after oxaliplatin treatment. The extent of genotoxicity was assessed by multiple genotoxicity assays (8-hydroxydeoxy-guanosine marker, comet, micronucleus and chromosomal aberration assays, oxidative stress-marker Glutathione evaluation) in order to determine diverse kinds of DNA damage. The results indicated that naringenin administration significantly reduced the DNA damage induced by oxaliplatin possibly due to its strong anti-oxidant properties. The results suggest that naringenin is a potential candidate for future development as a chemoprotective agent against chemotherapy associated complications.

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Section: Discussionsupporting

confidence: 78%

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

Ganaie

Jan

Khan

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Concerning the ovaries, iron excess can directly suppress female gonadal function [ 6 ]. Both substrates of the Fenton reaction (Fe 2+ and H 2 O 2 ) can be used to experimentally induce oxidative damage in macromolecules, such as lipids, protein, and DNA [ 7 , 8 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates

Rynkowska

Stępniak

Karbownik-Lewińska

2020

IJERPH

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The Fenton reaction (Fe2++H2O2→Fe3++•OH+OH-) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H2O2) are in excess. This study aims at comparing Fe2++H2O2-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17β-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO4+H2O2 with/without 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO4 concentrations (2400, 1200, 600, 300, 150 µM); (2nd) FeSO4 (2400, 1200, 600, 300, 150 µM)+H2O2 with/without 17β-estradiol; three experiments were performed with three highest 17β-estradiol concentrations; (3rd) FeSO4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 µM)+H2O2 (5 mM). LPO level [MDA+4-HDA/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe2+ concentrations (2400, 1200, 1100 µM). Exogenous 17β-estradiol (1 mM, 100, and 10 µM) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17β-estradiol activity. The therapeutic effect of 17β-estradiol against iron overload consequences should be considered with relation to both tissues.

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“…Estradiol could induce granulose cella and cumulus cell functional changes (Couse et al, 2005). Stepniak & Karbownik-Lewinska (2016) found that under physiological conditions estradiol contributed to protecting the ovary against oxidative damage to membrane lipids and nuclear DNA in porcine ovary. Coinciding with the previous studies, the present study indicated that melatonin stimulated the secretion of progesterone and estradiol in COCs, and the progesterone and estradiol production was higher in the 30 pmol mL −1 melatonin-treated group.…”

Section: Discussionmentioning

confidence: 97%

The effects of melatonin on bovine uniparental embryos developmentin vitroand the hormone secretion of COCs

Wang

Bao-ru

Liu

et al. 2017

PeerJ

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Melatonin is a unique multifunctional molecule that mediates reproductive functions in animals. In this study, we investigated the effects of melatonin on bovine parthenogenetic and androgenetic embryonic development, oocyte maturation, the reactive oxygen species (ROS) levels in parthenogenetic and androgenetic embryos and cumulus—oocyte complexes (COCs) hormone secretion with melatonin supplementation at four concentrations (0, 10, 20, and 30 pmol/mL), respectively. The results showed that melatonin significantly promoted the rates of bovine parthenogenetic and androgenetic embryonic cleavage and morula and blastocysts development (P < 0.05). The rate of cleavage was higher in the androgenetic embryo than that in the parthenogenetic embryo. Compared with the parthenogenetic embryos, the androgenetic embryos had a poor developmental competence from morula to blastocyst stage. Moreover, the levels of ROS were significantly lower in the parthenogenetic and androgenetic embryoes with melatonin-treated group than that of the control group (P < 0.05). Melatonin supplemented significantly increased the maturation rate of oocyte in vitro (P < 0.05). More importantly, melatonin significantly promoted the secretion of progesterone and estradiol by COCs (P < 0.05). To reveal the regulatory mechanism of melatonin on steroids synthesis, we found that steroidogenic genes (CYP11A1, CYP19A1 and StAR) were upregulated, suggesting that melatonin regulated estradiol and progesterone secretion through mediating the expression of steroidogenic genes (CYP11A1, CYP19A1 and StAR). In addition, MT1 and MT2 were identified in bovine early parthenogenetic and androgenetic embryos using western blot. It could be concluded that melatonin had beneficial effects on bovine oocyte in vitro maturation, COC hormone secretion, early development of subsequent parthenogenetic and androgenetic embryos. It is inferred that melatonin could be used to enhance the efficiency of in vitro developed embryos.

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17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary

Cited by 33 publications

References 37 publications

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates

The effects of melatonin on bovine uniparental embryos developmentin vitroand the hormone secretion of COCs

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