16S rRNA gene-based identification of cultured bacterial flora from host-seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna ticks, vectors of vertebrate pathogens

Rudolf, Ivo; Mendel, Jan; Šikutová, Silvie; Švec, Pavel; Masaříková, Jana; Nováková, Dana; Buňková, Leona; Sedláček, Ivo; Hubálek, Zdeněk

doi:10.1007/s12223-009-0059-9

Cited by 47 publications

(42 citation statements)

References 16 publications

(11 reference statements)

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“…This finding needs further experimental investigation to confirm that it relates to a specific pathogenic feature. Different species of bacteria that have been associated to other organisms were also characterized, including Vibrio rotiferianus associated with normal microbiota of rotifers (Gomez-Gil et al, 2003), and Microbacterium oxydans and Nocardiopsis alba associated with normal microbiota of different insects (Pidiyar et al, 2004;Rudolf et al, 2009;Patil et al, 2010). Other bacterial species involved in nosocomial infections were isolated, such as Stenotrophomonas rhizophila (normally associated with plants) (Wolf et al, 2002) and Stenotrophomonas maltophilia (Waters et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

et al. 2011

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Coral reefs are deteriorating at an alarming rate mainly as a consequence of the emergence of coral diseases. The white plague disease (WPD) is the most prevalent coral disease in the southwestern Caribbean, affecting dozens of coral species. However, the identification of a single causal agent has proved problematic. This suggests more complex etiological scenarios involving alterations in the dynamic interaction between environmental factors, the coral immune system and the symbiotic microbial communities. Here we compare the microbiome of healthy and WPD-affected corals from the two reef-building species Diploria strigosa and Siderastrea siderea collected at the Tayrona National Park in the Caribbean of Colombia. Microbiomes were analyzed by combining culture-dependent methods and pyrosequencing of 16S ribosomal DNA (rDNA) V5-V6 hypervariable regions. A total of 20 410 classifiable 16S rDNA sequences reads were obtained including all samples. No significant differences in operational taxonomic unit diversity were found between healthy and affected tissues; however, a significant increase of Alphaproteobacteria and a concomitant decrease in the Beta-and Gammaproteobacteria was observed in WPD-affected corals of both species. Significant shifts were also observed in the orders Rhizobiales, Caulobacteriales, Burkholderiales, Rhodobacterales, Aleteromonadales and Xanthomonadales, although they were not consistent between the two coral species. These shifts in the microbiome structure of WPD-affected corals suggest a loss of community-mediated growth control mechanisms on bacterial populations specific for each holobiont system. The ISME Journal (2012) 6, 502-512; doi:10.1038/ismej.2011.123; published online 29 September 2011Subject Category: microbial population and community ecology Keywords: bacterial community; white plague disease; coral diseases; pyrosequencing; Diploria strigosa; Siderastrea siderea IntroductionCoral reefs posses an immense biodiversity comparable only to that of the tropical rain forest (Mulhall, 2009). The structural, physiological and ecological bases of reefs are the scleractinian corals. They are in symbiotic relationship with a variety of bacteria and archaea as well as with microalgae (zooxanthellae), which are mainly responsible for the corals' high contribution to primary productivity of coral reefs . Modifications in the structure and relative density of symbiotic microbial communities might have a critical role in the coral adaptation to rapid environmental changes (Reshef et al., 2006). In the last three decades, local and global deterioration of environmental conditions have dramatically compromised the health of corals, which consequently affected the entire coral reef ecosystem. (Harvell et al., 1999(Harvell et al., , 2007Wilkinson, 1999;Green and Bruckner, 2000;Gardner et al., 2003;Pandolfi et al., 2003;Lesser et al., 2007). Recent reports indicate that 58-70% of coral reefs worldwide are threatened by human activities, while more than 30% of the biota associated with Caribb...

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Section: Discussionmentioning

confidence: 99%

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

et al. 2011

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“…(Mikryukova et al 2014). The hosts of adult H. concinna are cattle, goats, camels, sheep, pigs, horses, hedgehogs, rabbits, and wild boar (Rudolf et al 2009 …”

Section: Introductionmentioning

confidence: 99%

First detection of severe fever with thrombocytopenia syndrome virus in the tick species Haemaphysalis concinna in Shandong Province, China

et al. 2015

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The aim of this study was to detect severe fever with thrombocytopenia syndrome virus (SFTSV) infection using polymerase chain reaction (PCR) amplification in adult Haemaphysalis concinna ticks. A total of 72 adult H. concinna ticks were obtained from 35 goats, three adult H. concinna ticks (4.17 %) collected from two goats were found to be infected with SFTSV via PCR assay. Sequence analysis showed that the partial segment M glycoprotein gene of SFTSV was about 500 bases long by polymerase chain reaction (PCR) amplification and that the PCR products from the samples had an identical sequence (KP714259). With regard to the phylogenetic analysis, the Nei-Gojobri (Kimura 2-parameter) method was used to construct the phylogenetic trees. Phylogenetic analysis indicated that the obtained sequence closely resembled SFTSV strain from Zhejiang Province (KC189856) and belonged to the same clade. The similarity of these strains was up to 96.62 % (only differing by 17 bases). In addition, phylogenetic analysis also indicated that the sequence obtained from adult H. concinna ticks was most closely related to the sequence isolated from Haemaphysalis longicornis (KF781498) with 97.22 % similarity (differing only by 4 bases) and belonged to the same clade.

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“…have been described but their potential pathogenic nature remains to be investigated [44]. The Wolbachia and Arsenophonus genera are also bacteria associated with arthropods (mostly insects) and influence reproduction and/or immunity of their hosts [45,46]. They have also been found associated with ticks [47].…”

Section: The Technology-driven Revolution Of Tickborne Pathogen's Vismentioning

confidence: 99%

Emerging Horizons for Tick-Borne Pathogens: From the ‘One Pathogen–one Disease’ Vision to the Pathobiome Paradigm

et al. 2015

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Ticks, as vectors of several notorious zoonotic pathogens, represent an important and increasing threat for human and animal health in Europe. Recent applications of new technology revealed the complexity of the tick microbiome, which may affect its vectorial capacity. Appreciation of these complex systems is expanding our understanding of tick-borne pathogens, leading us to evolve a more integrated view that embraces the 'pathobiome'; the pathogenic agent integrated within its abiotic and biotic environments. In this review, we will explore how this new vision will revolutionize our understanding of tickborne diseases. We will discuss the implications in terms of future research approaches that will enable us to efficiently prevent and control the threat posed by ticks.

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16S rRNA gene-based identification of cultured bacterial flora from host-seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna ticks, vectors of vertebrate pathogens

Cited by 47 publications

References 16 publications

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

Shifts in bacterial communities of two caribbean reef-building coral species affected by white plague disease

First detection of severe fever with thrombocytopenia syndrome virus in the tick species Haemaphysalis concinna in Shandong Province, China

Emerging Horizons for Tick-Borne Pathogens: From the ‘One Pathogen–one Disease’ Vision to the Pathobiome Paradigm

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