16S rRNA Amplicon Sequencing of Microbial Biofilms from Marsberg Copper Mine, Germany

Abstract

The 16S rRNA amplicons from biofilms inhabiting rocks near various water bodies of Marsberg Copper Mine (Rhenish Massif, Germany) reveal the diversity of their microbial communities. The abundance of Chloroflexi and Cyanobacteria taxa in the biofilms near leachate streams indicated the selective enrichment of Ktedonobacteria and Oxyphotobacteria members.

Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif

“…Data availability. The datasets related to relative abundance graphs and hydrochemistry are accessible at https://doi.org/10.25625/DFFZ9R (Arif, 2021b). The pathway maps of the MAGs can also be assessed at Göttingen Research Online, https://doi.org/10.25625/W9PWCX (Arif, 2021c).…”

“…2 [(MBS1-MBS4); (MB1-MB6, MBS18); (MBS10, 11, 13)] were divided into spring water, leachate, and unconsolidated rocks groups, respectively, based on the nearby drainage water bodies. A detailed description of the biological features and origin of the collected samples has been published previously (Arif et al, 2021b). Biofilm and water samples grouped as spring water samples (MBS1-MBS4) were taken along a small karst ground water stream that springs up in a cased basin and then naturally flows inside the Kilianstollen main gallery.…”

“…Based on the Bray-Curtis index, in principal coordinates analysis (PCoA), the data representing similarities of complex microbial communities were plotted into 2D and 3D graphs. Amplicon sequencing data have been published to the Sequence Read Archive (SRA) as SRR12876542-SRR12876555 under the project accession number PRJNA670497, as announced previously (Arif et al, 2021b).…”

Composition and Niche-Specific Characteristics of Microbial Consortia Colonizing Marsberg Copper Mine in the Rhenish Massif

“…2. A detailed description of the biological features and origin of the collected samples has been published previously (Arif et al, 2021b). The biofilm and water samples were taken along the natural flowing water streams in the Kilianstollen mine, for instance, the biofilms (MBS1-4 grouped as spring water biofilms) were collected around the spring freshwater stream which was gradually enriched in calcium, carbonate, and sulphate ions with the flow streams from the crosscut that branches off from the rear course of the mine (Fig.…”

“…Based on the Bray-Curtis index, in principal coordinates analysis (PCoA) the data representing similarities of complex microbial communities were plotted into 2D and 3D graphs. Amplicon sequencing data has been published to the Sequence Read Archive (SRA) as SRR12876542-SRR12876555 under the Project accession number PRJNA670497 as announced previously (Arif et al, 2021b).…”