16S rDNA analysis of the effect of fecal microbiota transplantation on pulmonary and intestinal flora

Abstract

This study aims to explore the effect of FMT on regulations of dysbacteriosis of pulmonary and intestinal flora in rats with 16S rDNA sequencing technology. A total of 27 SPF rats (3-4 weeks old) were randomly divided into three groups: normal control group (K), model control group (MX), and fecal microbiota transplantation group (FMT); each group contained nine rats. The OTU values of the pulmonary and intestinal flora of the MX group decreased significantly compared with the normal control group. After FMT, the OTU value of pulmonary flora increased, while the value of OTU in intestinal flora declined. At the phylum level, FMT down-regulated ,, and in the pulmonary flora. At the genus level, FMT down-regulated, ,, , and, thus maintaining the balance of the pulmonary flora. Moreover, FMT could change the structure and diversity of the pulmonary and intestinal flora by positively regulating the pulmonary flora and negatively regulating intestinal flora. This study may provide a scientific basis for FMT treatment of respiratory diseases.

Effects of Bacillus subtilis PB6 and/or chromium propionate supplementation on clinical health, growth performance, and carcass traits of high-risk cattle during the feedlot receiving and finishing periods1

“…The composition of the gut and lung microbiome are correlated and remain so throughout the life span, indicative of a host-wide microbial network ( Grier et al, 2018 ). For example, Madan et al (2012) and Liu et al (2017) reported that fecal transplantation of rats induced changes in the lung microbiome. Likewise, mice challenged with exogenous lipopolysaccharide to the lungs consistently experience disturbance of their gut microbiome ( Sze et al, 2012 ).…”

The Gut-Lung Axis in Health and Respiratory Diseases: A Place for Inter-Organ and Inter-Kingdom Crosstalks

“…From birth throughout the entire life span, a close correlation between the composition of the gut and lung microbiota exists, suggesting a host-wide network (Grier et al, 2018). For instance, modification of newborns' diet influences the composition of their lung microbiota, and fecal transplantation in rats induces changes in the lung microbiota (Madan et al, 2012;Liu et al, 2017).…”

Impact of Amoxicillin-Clavulanate followed by Autologous Fecal Microbiota Transplantation on Fecal Microbiome Structure and Metabolic Potential

“…Additionally, microbiota structure varies significantly between individuals, and a poor donor-recipient match may lead to dysbiosis or FMT failure ( 23 – 26 ). Studies to date of the effects of FMT on the microbiome have primarily relied on 16S ribotype-based analyses of bacterial taxonomic composition and diversity ( 27 – 30 ). While such studies have been transformative in demonstrating how well taxonomic compositions match between FMT donors and recipients over time, they are generally not designed to illuminate functional changes in the microbiome ( 29 , 30 ).…”

“…Studies to date of the effects of FMT on the microbiome have primarily relied on 16S ribotype-based analyses of bacterial taxonomic composition and diversity ( 27 – 30 ). While such studies have been transformative in demonstrating how well taxonomic compositions match between FMT donors and recipients over time, they are generally not designed to illuminate functional changes in the microbiome ( 29 , 30 ). Accordingly, complementary approaches are required to enable a higher-resolution understanding of the impact of FMTs on the composition, dynamics, and transmission of resistance genes and metabolic capacity encoded by the microbiome.…”