16O2/18O2 analysis of oxygen exchange in Dunaliella tertiolecta. Evidence for the inhibition of mitochondrial respiration in the light

Bate, G. C.; Sültemeyer, Dieter; Fock, Heinrich P.

doi:10.1007/bf00028841

Cited by 21 publications

(11 citation statements)

References 26 publications

(46 reference statements)

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“…Under illumination, mitochondrial respiration may be down-regulated because photosynthesis supplies sufficient amounts of ATP and NADPH or the enzymes of the TCA are potentially inactivated (Loreto et al 2001;Pinelli and Loreto 2003). Some reports based on O 2 consumption suggest an inhibition of respiration in light conditions (Canvin et al 1980;Bate et al 1988), whereas others suggest that the rate of respiration is invariant, regardless of illumination conditions (Gerbaud and Andre 1980). Based on our current analysis, we hypothesized that this phenomenon is due to the inhibition of mitochondrial respiration by light in the mutant, as chlorina-type plant leaves have no PS II activity.…”

Section: Discussionmentioning

confidence: 97%

Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

Goh

Satoh

Kikuchi

et al. 2010

Plant Biotechnol Rep

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The rice CHLH gene encodes the Mg 2? -chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves during oschlh seedling development. Growth of mutant plants was enhanced in the presence of 3% sucrose, which may be used by mitochondria to meet cellular energy requirements. ATP content in these mutants was, however, significantly lowered in light conditions. Low cytosolic levels of NADH in illuminated oschlh mutant leaves further indicated the inhibition of mitochondrial metabolism. This down-regulation was particularly evident for oxidative stressresponsive genes in the mutant under light conditions. Hydrogen peroxide levels were higher in oschlh mutant leaves than in wild-type leaves; this increase was largely caused by the impairment of the expression of the antioxidant genes, such as OsAPX1, OsRAC1, and OsAOXc in knockout plants. Moreover, treatment of mesophyll protoplasts with ascorbic acid or catalase recovered ATP content in the mutants. Taken together, these results suggest that the light-mediated inhibition of mitochondrial activity leads to stunted growth of CHLH rice seedlings.

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Section: Discussionmentioning

confidence: 97%

Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

Goh

Satoh

Kikuchi

et al. 2010

Plant Biotechnol Rep

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“…However, although it can be inhibited (e.g. Bate et al 1988) or stimulated (e.g. Weger et al 1989), the current consensus is that it is similar in the dark and light (Geider 1992).…”

Section: Respiration In the Light And Darkmentioning

confidence: 99%

Oxygen production and carbon fixation in oligotrophic coastal bays and the relationship with gross and net primary production

González¹,

Gattuso²,

Middelburg³

2008

Aquat. Microb. Ecol.

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Planktonic primary production and respiration in 2 coastal oligotrophic sites of the Northwest Mediterranean Sea were examined. Primary production was quantified using 3 methods (light and dark changes in dissolved O 2 , 18 O-labeling and 14 C uptake technique) using in situ bottle incubations. Gross primary production (GPP) based on the O 2 light-dark technique was not significantly different from that using the 18 O-labeling technique, indicating that the former technique provides accurate estimates of GPP in these environments. Respiration in the dark was not significantly different from respiration in the light. Total 14 C uptake (including the dissolved and particulate organic carbon fractions) during the whole duration of the light period approached GPP and consequently overestimated net primary production.

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“…For cases in which reoxidation of NADH in mitochondria is an essential part of the photorespiratory carbon [entry (5)], the Fe cost of this reoxidation (see Raven, 1988) is included in the Fe entries. (Sultemeyer, Klug & Fock, 1986Miller, Espie & Canvin, 1988 a, b;Sultemeyer, Stuhlfauth & Fock, 1988) this is not the case for other algae and cyanobacteria which pump, or reasonably may be assumed to pump, COg (Sultemeyer, Stuhlfauth & Fock, 1987;Bate, Sultemeyer & Fock, 1988;Weger et al, 1989;cf. Zenvirth & Kaplan, 1981;Burns & Beardall, 1987).…”

Section: Predicted Iron and Manganese Use Efficiency Of Light-satmentioning

confidence: 99%

Predictions of Mn and Fe use efficiencies of phototrophic growth as a function of light availability for growth and of C assimilation pathway

1990

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SUMMARY Iron is involved in many photosynthetic, respiratory and nitrogen assimilation reactions of plants as Fe bound tightly to polypeptides catalysing redox reactions. Manganese is involved as tightly bound Mn in photoreaction II of photosynthesis and in certain superoxide dismutases, while loosely bound Mn2+ is the unique activator of some enzymes, and is an alternative to Mg2+ in activating many enzymes. This paper uses data on the quantitative role of Fe and Mn in catalysts to predict the efficiency with which Fe and Mn are used in C assimilation [mol C assimilated (mol catalytic metal in enzyme)−1 s−1] and the metal cost of C assimilation [mol catalytic metal in enzyme (mol C assimilated)−1 s−1] in photolithotrophic growth in relation to genetic and environmental variables. The genetic variables were the relative content of thylakoid proteins in major taxa (cyanobacteria and red algae, chlorophytes and chromophytes) and smaller‐scale taxonomic differences (various subtypes of C4 metabolism, and C3 metabolism, in terrestrial vascular plants). The environmental variables were the range of photon flux densities in which photolithotrophic growth of O2 evolvers can occur, and the inorganic C supply conditions controlling the repression/de‐repression of the inorganic C concentrating mechanism in cyanobacteria and microalgae. The results of the computations yield the following conclusions. The largest predicted difference in Fe and Mn costs of photolithotrophic growth is related to changes in the photon flux density for growth. The predicted Fe cost increased 50‐fold, and the Mn cost increased 80‐fold, at the lowest extreme of photon flux density compared to the highest found naturally. The increase is partly countered by the larger ratio of light‐harvesting pigments to thylakoid protein complexes assumed for the cells grown at low photon flux densities, although the extent of the increase in photosynthetic unit size is limited by considerations of efficiency of excitation energy transfer. However, the major influences are the higher pigment content in biomass enabling a larger fraction of incident light to the absorbed, and the sub‐maximal specific reaction rates of redox catalysts (whose content is constrained via excitation energy transfer considerations) at very low photon flux densities. A smaller difference, four‐fold or less, in Fe and Mn costs of photolithotrophic growth, is predicted by comparing major taxa (cyanobacteria plus red algae; chlorophytes plus chromophytes) with contrasting ratios of thylakoid redox catalysts. Differences in Fe and Mn costs of growth of less than two‐fold are predicted when the Fe‐ and Mn‐efficient organisms with CO2− concentrating mechanisms (C4 land plants; algae with active inorganic C influx) and high requirements for ATP relative to NADPH, are compared with organisms relying on CO2 diffusion from air or air‐equilibrated solutions and C3 biochemistry. These predictions of variations in Fe and Mn costs of photolithotrophy have implications for the ecology of phototrophs.

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16O2/18O2 analysis of oxygen exchange in Dunaliella tertiolecta. Evidence for the inhibition of mitochondrial respiration in the light

Cited by 21 publications

References 26 publications

Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

Oxygen production and carbon fixation in oligotrophic coastal bays and the relationship with gross and net primary production

Predictions of Mn and Fe use efficiencies of phototrophic growth as a function of light availability for growth and of C assimilation pathway

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