161 Hepatoprotective Effect of Citrulline Against Ischemia-Reperfusion Injury in Rat

Moussaoui, F.; Binimbi, A.; Cottart, Charles‐Henry; Amen, N.; Vamy, Michel; Nivet-Antoine, Valérie; Cynober, Luc; Bandt, J.-P. De

doi:10.1016/s0168-8278(09)60163-x

Cited by 5 publications

(5 citation statements)

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“…Finally, Cit is involved in the regulation of protein and energy metabolism (16) because it activates muscle protein synthesis (17) and improves insulin sensitivity. Moreover, Cit also displays antioxidative properties, as previously shown by our group in a model of in vivo ischemia-reperfusion of the liver (18). Therefore, Cit may be able to restore hepatic energy metabolism, which plays a major role in the development of NAFLD.…”

Section: Introductionsupporting

confidence: 61%

Citrulline and Nonessential Amino Acids Prevent Fructose-Induced Nonalcoholic Fatty Liver Disease in Rats

Jegatheesan

Beutheu

Ventura

et al. 2015

The Journal of Nutrition

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Section: Introductionsupporting

confidence: 61%

Citrulline and Nonessential Amino Acids Prevent Fructose-Induced Nonalcoholic Fatty Liver Disease in Rats

Jegatheesan

Beutheu

Ventura

et al. 2015

The Journal of Nutrition

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“…Moussaoui et al did not utilize GGT as part of their liver integrity assessment. However, they found that CIT significantly reduced elevations of all serum biomarkers, in which the present study failed to demonstrate [15]. Jegatheesan et al used alkaline phosphatase (ALP), a marker of hepatobiliary injury similar to GGT; however, they also found that CIT only caused insignificant attenuation of ALP elevation [16].…”

Section: Discussioncontrasting

confidence: 81%

“…This finding is in agreement with those of other studies evaluating CIT hepatoprotective ability. Moussaoui et al reported on rats with modeled ischemia/reperfusion injury and found that CIT treatment significantly reduced elevations of ALT and AST (p<0.05), along with lactate dehydrogenase, arginase, and intrahepatic myeloperoxidase activity [15]. In addition, Jegatheesan et al identified a significant decrease of plasma ALT levels due to CIT in rats with fructose-induced liver steatosis.…”

Section: Discussionmentioning

confidence: 98%

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Hepatoprotective Effects of L-Citrulline Against Doxorubicin-Induced Liver Damage in Rats: An Analysis of Serum Biomarkers

et al. 2019

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Objective: The antineoplastic agent doxorubicin (DOX) is known for causing liver toxicity. Its metabolism in hepatocytes causes oxidative stress, which, in turn, induces DNA damage, lipid peroxidation, ATP depletion, and apoptosis. L-citrulline (CIT), a commonly found agent in fruits like watermelon, has piqued interest due to its antioxidant properties. In the body, CIT is converted to nitric oxide, which has been shown to mitigate hepatic injury by scavenging free radicals, improving hepatic sinusoidal microcirculation, and inhibiting neutrophilic infiltration. This study aims to investigate CIT ability to prevent DOX-induced hepatotoxicity.Methods: A total of 20 Wistar rats were randomized to receive either DOX (10 mg/kg BW) or NaCl 0.9%. DOX-intoxicated group was further randomized to either received low-dose CIT (300 mg/kg BW), high-dose CIT (600 mg/kg BW), or aquadest. CIT was given orally for 6 days and DOX through intraperitoneal injection on days 4 and 5. Serum was obtained and hepatotoxicity was assessed with serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT). Statistical analysis was done with one-way ANOVA and Tukey's test.Results: Serum ALT, AST, and GGT were increased significantly compared to that of normal group. CIT administration in both the doses could decrease the serum levels of ALT and AST significantly compared to that of DOX group. In this study, CIT in both the doses could reduce the serum levels of GGT compared to that of DOX group though not statistically significant. Conclusions:This study suggests that CIT exerts hepatoprotective effect, as evident by the attenuation of serum biomarkers.

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“…As a consequence, Cit significantly decreased liver weight compared with the WD group. The effect of Cit on lipid metabolism may be the result of decreased de novo lipogenesis, but also to a better VLDL-TAG release given the improved peripheral TAG oxidation previously demonstrated ( 29 ) .…”

Section: Discussionmentioning

confidence: 96%

Preventive effects of citrulline on Western diet-induced non-alcoholic fatty liver disease in rats

et al. 2016

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A Western diet induces insulin resistance, liver steatosis (non-alcoholic fatty liver disease (NAFLD)) and intestinal dysbiosis, leading to increased gut permeability and bacterial translocation, thus contributing to the progression of NAFLD to non-alcoholic steatohepatitis. In the present study, we sought, in a model of Western diet-induced NAFLD, to determine whether citrulline (Cit), an amino acid that regulates protein and energy metabolism, could decrease Western diet-induced liver injuries, as well as the mechanisms involved. Sprague-Dawley rats were fed a high-fat diet (45 %) and fructose (30 %) in drinking water or a control diet associated with water (group C) for 8 weeks. The high-fat, high-fructose diet (Western diet) was fed either alone (group WD) or with Cit (1 g/kg per d) (group WDC) or an isonitrogenous amount of non-essential amino acids (group WDA). We evaluated nutritional and metabolic status, liver function, intestinal barrier function, gut microbiota and splanchnic inflammatory status. Cit led to a lower level of hepatic TAG restricted to microvesicular lipid droplets and to a lower mRNA expression of CCAAT-enhancer-binding protein homologous protein, a marker of endoplasmic reticulum stress, of pro-inflammatory cytokines Il6 (P < 0·05) and Tnfα, and of toll-like receptor 4 (Tlr4) (P < 0·05). Cit also improved plasma TAG and insulin levels. In the colon, it decreased inflammation (Tnfα and Tlr4 expressions) and increased claudin-1 protein expression. This was associated with higher levels of Bacteroides/Prevotella compared with rats fed the Western diet alone. Cit improves Western diet-induced liver injuries via decreased lipid deposition, increased insulin sensitivity, lower inflammatory process and preserved antioxidant status. This may be related in part to its protective effects at the gut level.

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161 Hepatoprotective Effect of Citrulline Against Ischemia-Reperfusion Injury in Rat

Cited by 5 publications

References 0 publications

Citrulline and Nonessential Amino Acids Prevent Fructose-Induced Nonalcoholic Fatty Liver Disease in Rats

Citrulline and Nonessential Amino Acids Prevent Fructose-Induced Nonalcoholic Fatty Liver Disease in Rats

Hepatoprotective Effects of L-Citrulline Against Doxorubicin-Induced Liver Damage in Rats: An Analysis of Serum Biomarkers

Preventive effects of citrulline on Western diet-induced non-alcoholic fatty liver disease in rats

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