16 Progesterone Release Patterns From Cue-Mate in Comparison to Other Intravaginal Progesterone-Releasing Devices in Lactating Dairy Cows

Abstract

Various intravaginal progesterone-releasing devices are commercially available and each is impregnated with different amounts of progesterone. An experiment was designed to characterize plasma progesterone release profiles from Cue-Mate in comparison with other commercially available progesterone-releasing devices. Cycling, lactating Holstein cows (n = 60; BCS between 2.0 and 3.0 out of 5) from commercial dairy farms in Argentina were used in 2 replicates. All cows received 2 injections of D+ cloprostenol (PGF, Bioprost-D�; Biotay, Buenos Aires, Argentina) 14 days apart and were randomly assigned to one of four groups to receive intravaginal devices as follows: Cue-Mate (1.56 g progesterone; Bioniche Animal Health, A/Asia Pty, Armidale, Australia; n = 10), DIB (1 g progesterone; Syntex SA, Argentina; n = 10), CIDR with 1.9 g progesterone (Pfizer Salud Animal S.A., Buenos Aires, Argentina; n = 10), or CIDR with 1.38 g progesterone (Pfizer Animal Health, Groton, CT, USA; n = 10). All devices were inserted 24 hours after the second PGF and were left in place for 31 days. Blood samples were taken for progesterone analysis at 6-hour intervals for the first 24 hours, then daily for a week, and every 2 days for an additional 25 days. Plasma progesterone concentrations were measured with a modified human double-antibody RIA kit (DPC Coat-A-Count�; Diagnostic Products Corporation, Los Angeles, CA, USA). Cows were observed twice daily to confirm that vaginal inserts were still in place. Time-series hormone data were analyzed using the MIXED procedure for repeated measures (SAS Institute, Inc., Cary, NC, USA). Main effects of device and time, the highest mean concentration of progesterone, and the mean day on which this occurred were compared by a protected LSD test. There was no evidence of cow discomfort with any of the vaginal devices, and loss rates did not differ. There was an effect of group (P < 0.001) and day (P < 0.001) for progesterone concentrations, but there was no interaction (P = 0.89). Peak concentrations of progesterone (ng mL-1) did not differ among groups (Cue-Mate: 5.0 � 1.1; DIB: 3.9 � 0.6; 1.9-g CIDR: 4.6 � 0.6; 1.38-g CIDR: 3.7 � 0.4; P = 0.51). Mean (� SEM) concentrations of progesterone over the 31-day insertion period differed between 1.9-g CIDR (1.8 � 0.2 ng mL-1) and DIB (1.5 � 0.1 ng mL-1, P < 0.006), with Cue-Mate (1.5 � 0.2 ng mL-1) and 1.38-g CIDR (1.6 � 0.1 ng mL-1) intermediate and not different from either. When data were evaluated from insertion until Day 7, mean plasma progesterone concentrations did not differ among groups (Cue-Mate: 2.2 � 0.2 ng mL-1; DIB: 2.2 � 0.1 ng mL-1; 1.9-g CIDR: 2.6 � 0.2 ng mL-1; 1.38-g CIDR: 2.1 � 0.1 ng mL-1). However, progesterone concentrations from insertion until Day 21 were higher (P < 0.03) in cows with a 1.9-g CIDR (2.1 � 0.1 ng mL-1) than in those with a DIB (1.6 � 0.1 ng mL-1) or a 1.38-g CIDR (1.6 � 0.1 ng mL-1), with Cue-Mate (1.7 � 0.1 ng mL-1) intermediate and not different. Based on progesterone release patterns, it was concluded that Cue-Mate intravaginal devices can be used in protocols for synchronization of estrus and ovulation as with other progesterone-releasing devices.

Does the Amount of Progesterone in Intravaginal Implants Used to Synchronise Oestrus Affect the Reproductive Performance of Brahman Heifers Artificially Inseminated at a Fixed Time

“…There were no significant differences between treatment groups in the mean progesterone concentrations of the monitor heifers on each sampling day, confirming results by Rogan et al. (2007) that compared circulating progesterone concentrations of four different implants (implants concentration ranged from 1.0 to 1.9 g P 4 ).…”

Reproductive performance of cows after fixed‐time artificial insemination with ovulation synchronisation and re‐synchrony in southern Australian dairy herds

“…Our results were consistent with previously published work in New Zealand anoestrous dairy cows: there was no significant difference in conception rates when comparing the use of a 1.0 g progesterone DIB‐V with a 1.38 g progesterone CIDR® in a FTAI program . Similarly in another study, no differences in peak or mean progesterone concentrations were found in lactating Holstein cows when treated with the 1.9‐g CIDR®, 1.56‐g Cuemate®, 1.38‐g CIDR or 1.0‐g DIB‐V® devices . The use of intravaginal progesterone devices in GnRH‐based programs to improve conception rates has been shown to be most effective in non‐cycling cows .…”

Effect of post artificial insemination treatment with two different progesterone intravaginal devices on conception and synchronization of the returning estrus in Japanese Black cows

“…This result also showed the effect of DIB treatment on plasma P4 concentration was similar to CIDR treatment in Japanese Black cows. This study coincided with the previous report that 7-days treatment with DIB or CIDR for estrus synchronization resulted in similar mean plasma P4 concentration in dairy cows [32], although different P4 doses of CIDR affected plasma P4 concentration in CIDR-synch protocol [19]. In this study, though P4 values on Day 19 were significantly higher in pregnant cows than in non-pregnant cows in DIB and control group, it did not differ in CIDR group.…”