1999
DOI: 10.1016/s0076-6879(99)03018-9
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[16] A modified method for the display of 3′-end restriction fragments of cDNAs: Molecular profiling of gene expression in neutrophils

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Cited by 23 publications
(23 citation statements)
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“…17 Human hematopoietic stem and progenitor cell populations were isolated from peripheral blood stem cells (mobilized by granulocyte colonystimulating factor), and monocytes were prepared from peripheral blood, as previously described. [18][19][20] The University of Massachusetts Medical School and Yale University human subjects committees approved procedures and consent forms at their respective sites.…”
Section: Human Leukocyte Isolationmentioning
confidence: 99%
“…17 Human hematopoietic stem and progenitor cell populations were isolated from peripheral blood stem cells (mobilized by granulocyte colonystimulating factor), and monocytes were prepared from peripheral blood, as previously described. [18][19][20] The University of Massachusetts Medical School and Yale University human subjects committees approved procedures and consent forms at their respective sites.…”
Section: Human Leukocyte Isolationmentioning
confidence: 99%
“…21 All reagents, serum, buffers, and media were free of LPS (less than 0.01 ng/mL by limulus amoebocyte lysate assay [Sigma]). Monocytes were enumerated in neutrophil preparations by flow microfluorometry.…”
Section: Cell Separation and Activation By Bacteriamentioning
confidence: 99%
“…cDNA was then synthesized using a T-7 Sal-Oligo d(T) 32 primer as described previously. 8,18 The double-stranded cDNA was digested with 1 of 9 different restriction enzymes (ApaI, BglII, BamHI, EagI, EcoRI, HindIII, XbaI, KpnI, and SphI) and ligated to Y-shaped adaptors with a complementary overhang. DNA fragments were then amplified by PCR as described previously.…”
Section: Rna Isolation and Differential Displaymentioning
confidence: 99%
“…DNA fragments were then amplified by PCR as described previously. 8,18 PCR products were separated on a sequencing gel of 6% polyacrylamide with 7 M urea. The gel was dried and exposed to x-ray film.…”
Section: Rna Isolation and Differential Displaymentioning
confidence: 99%
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