150-seed comparative longevity protocol – a reduced seed number screening method for identifying short-lived seed conservation collections

Davies, Rachael M.; Newton, Rosemary J.; Hay, Fiona R.; Probert, R. J.

doi:10.15258/sst.2016.44.3.13

Cited by 18 publications

(26 citation statements)

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“…In these countries, lower‐cost conservation techniques, such as cold chambers and freezers, are of particular interest, because cryopreservation techniques are more expensive and for this reason are often not available (Davies et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…These responses can be influenced by many factors, including intrinsic physiological characters of each species, and also seed structure, environmental factors (e.g. temperature, humidity and moisture content) and the degree of infection by fungi and bacteria (Rojas-Aréchiga & Vázquez-Yanes 2000; Walters et al 2005;Probert et al 2009;Davies et al 2016;Meiado et al 2016). Seeds of C. fernambucensis subsp.…”

Section: Discussionmentioning

confidence: 99%

“…We hope that our results will encourage the formation of seed banks and therefore bolster seed conservation, especially in developing countries, where most of the endangered plant species occur. In these countries, lower-cost conservation techniques, such as cold chambers and freezers, are of particular interest, because cryopreservation techniques are more expensive and for this reason are often not available (Davies et al 2016).…”

Section: Discussionmentioning

confidence: 99%

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Seed storage of Brazilian cacti species in different threat categories

Santos

Hassemer

Meiado

2018

Plant Species Biology

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Cactaceae is considered the fifth most endangered taxonomic group. In light of this, the aim of this study was to evaluate the efficiency of different low‐temperature storage techniques in maintaining the viability of seeds of cacti in different threat categories. Seeds of six cacti taxa were stored in a cold chamber (8°C), a freezer (−5°C), in liquid nitrogen (−196°C) and at room temperature (25–27°C) for a period of 0, 1, 3, 6, 9 and 13 months. At each evaluation interval we removed a seed sample for each taxon studied, which was distributed into four repetitions of 25 seeds maintained at room temperature under 12‐h light/dark photoperiods. We evaluated the germinability, mean germination time and synchronization index. Most of the studied taxa presented germinability of above 50%, which was influenced by time and by storage temperatures. Also, most taxa stored at room temperature presented a significant reduction in germinability, whereas almost all taxa showed maintenance of the seed viability when stored in a cold chamber, a freezer or liquid nitrogen. This response can be justified by the reduction of the seed metabolism and the degradation of the reserve compounds of the seeds while at lower temperatures. Our results indicate that storage at low temperatures is an effective method for the conservation of cacti seeds and can be used for the formation of artificial seed banks of threatened cacti species.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Seed storage of Brazilian cacti species in different threat categories

Santos

Hassemer

Meiado

2018

Plant Species Biology

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“…The individual aliquots of seeds are removed after 1, 2, 5, 9, 20, 30, 50, 75, 100, and 125 days (Newton et al 2014) or 2, 10, 15, and 30 days if the seed lot is expected to have poor longevity and few seeds available (Davies et al 2006) to test the ability of the seeds to germinate. This germination data is then analyzed, usually through probit analysis, to estimate the p 50 and provide an estimate for relative seed longevity (Figs.…”

Section: Box 2 Seed Storage Experimentsmentioning

confidence: 99%

Seed storage: maintaining seed viability and vigor for restoration use

Vitis

Hay

Dickie

et al. 2020

Restoration Ecology

115

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Effective seed storage after sourcing (harvesting or purchasing) is critical to restoration practitioners and native seed producers, as it is key to maintaining seed viability. Inadequate seed storage can lead to a waste of both natural and economic resources when seeds of poor quality are sown. When working with native species with unknown storage behavior, general assumptions can be made based on studies on related species, and standard practices may be applied with caution; however, an investigation should be conducted to understand if specific storage requirements are needed and for how long seeds can be stored before they lose significant viability. In this paper of the Special Issue Standards for Native Seeds in Ecological Restoration, we provide an overview of the key concepts in seed storage and the steps to take for effective storage of native seeds for restoration use.

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“…1). Emerging technologies and protocols that optimize staff time and costs, and at the same time reduce the number of seeds for testing, should be implemented to monitor viability in seed genebanks and in tissue cultures (Harding et al 2013;Davies et al 2016;Hay & Whitehouse 2017). Lastly, our nationwide survey has shown the need to improve data accessibility and science communication.…”

Section: Resultsmentioning

confidence: 99%

Ex situ conservation of threatened plants in Brazil: a strategic plan to achieve Target 8 of the Global Strategy for Plant Conservation

et al. 2018

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With increasing rates of habitat destruction and species loss, ex situ conservation is gaining global momentum and reluctance in relying on ex situ conservation is rapidly giving way to a more optimistic, strategic view. Target 8 of the Global Strategy of Plant Conservation calls for at least 75 percent of threatened plant species in accessible ex situ collections, preferably in the country of origin, and 20 percent of them included in recovery and restoration programs. Here, we provide updated information on Brazil's progress towards Target 8 through a nationwide examination of how many threatened species were conserved in ex situ collections in Brazil. Our data comprised whole plants (living collections), seed (seed banks) and tissue cultures (in vitro). Of the 2,113 threatened species, at least 452 (21.4%) species were conserved in ex situ collections, an increase in 4% of living organisms and 96% of seeds when compared to a previous assessment. Since it is unlikely Brazil will achieve Target 8 by 2020, we also discuss public policies and strategies to help overcome key bottlenecks preventing its achievement and propose revised goals for the GSPC 2020-2030.

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150-seed comparative longevity protocol – a reduced seed number screening method for identifying short-lived seed conservation collections

Cited by 18 publications

References 0 publications

Seed storage of Brazilian cacti species in different threat categories

Seed storage of Brazilian cacti species in different threat categories

Seed storage: maintaining seed viability and vigor for restoration use

Ex situ conservation of threatened plants in Brazil: a strategic plan to achieve Target 8 of the Global Strategy for Plant Conservation

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