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Annadana, S.; Beekwilder, Jules; Kuipers, G.J.; Visser, Peter B.; Outchkourov, Nikolay S.; Pereira, Andy; Udayakumar, M.; Jong, Jan de; Jongsma, Maarten A.

doi:10.1023/a:1016313924844

Cited by 29 publications

(5 citation statements)

References 15 publications

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“…Mounting evidence suggests that numerous cis-elements play a vital role in specifying the tissue expression patterns of plant genes 50,51 . To understand what drives the expression of SlOFP20 in the pollen, a 2000-bp region upstream of the SlOFP20 start codon was submitted to a public database (http://www.dna.affrc.go.jp/PLACE) to analyze cis-acting elements to determine whether pollen development-associated elements were present in the promoter.…”

Section: Resultsmentioning

confidence: 99%

Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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The OVATE gene was initially identified in tomato and serves as a key regulator of fruit shape. There are 31 OFP members in the tomato genome. However, their roles in tomato growth and reproductive development are largely unknown. Here, we cloned the OFP transcription factor SlOFP20. Tomato plants overexpressing SlOFP20 displayed several phenotypic defects, including an altered floral architecture and fruit shape and reduced male fertility. SlOFP20 overexpression altered the expression levels of some brassinosteroid (BR)-associated genes, implying that SlOFP20 may play a negative role in the BR response, similar to its ortholog OsOFP19 in rice. Moreover, the transcript accumulation of gibberellin (GA)-related genes was significantly affected in the transgenic lines. SlOFP20 may play an important role in the crosstalk between BR and GA. The pollen germination assay suggested that the pollen germination rate of SlOFP20-OE plants was distinctly lower than that of WT plants. In addition, the tomato pollen-associated genes SlCRK1, SlPMEI, LePRK3, SlPRALF, and LAT52 were all suppressed in the transgenic lines. Our data imply that SlOFP20 may affect floral organ and pollen development by modulating BR and GA signaling in tomato.

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Section: Resultsmentioning

confidence: 99%

Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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“…To date, many cis-elements have been reported for their critical roles in determining the tissue-specific expression profiles of plant genes 38 39 . In this study, a 1534-bp region upstream of the SlGLO1 start codon was analyzed to identify cis-acting elements, using the information in two public databases ( http://www.dna.affrc.go.jp/PLACE and http://intra.psb.ugent.be:8080/ Plant CARE).…”

Section: Resultsmentioning

confidence: 99%

The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development

Guo

Yin

et al. 2016

Sci Rep

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MADS-box transcription factors play important role in plant growth and development, especially floral organ identities. In our study, a MADS-box gene SlGLO1- tomato floral homeotic protein FBP1-like gene was isolated. Its tissue-specific expression profile analysis showed that SlGLO1 was highly expressed in petals and stamens. RNAi (RNA interference) repression of SlGLO1 resulted in floral organ abnormal phenotypes, including green petals with shorter size, and aberrant carpelloid stamens. SlGLO1-silenced lines are male sterile. Total chlorophyll content was increased and chlorophyll biosynthetic genes were significantly up-regulated in SlGLO1-silenced petals and stamens. Furthermore, B-class genes expression analysis indicated that the repressed function of SlGLO1 led to the enhanced expression of TAP3 and the down-regulation of TPI in the petals and stamens, while the expression of TM6 was reduced in petals and increased in stamens and carpels of SlGLO1-RNAi plants. Additionally, pollen grains of transgenic lines were aberrant and failed to germinate and tomato pollen-specific genes were down-regulated by more than 90% in SlGLO1-silenced lines. These results suggest that SlGLO1 plays important role in regulating plant floral organ and pollen development in tomato.

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“…Genomic DNA was extracted from silica gel-dried or fresh leaves with a Plant Genome Extraction Kit (Tiangen Biotech, China) following the manufacturer’s protocol. For better phylogenetic resolution, we utilized nrITS and seven low-copy nuclear genes, AGO1 ( ARGONAUTE 1 ; Zhang et al, 2015 ), BRC1 ( BRANCHED1 ; Zhou et al, 2012 ; Wang M. et al, 2019 ), CDS (chrysanthemyl diphosphate synthase gene; Rivera et al, 2001 ; Liu et al, 2012a ), F3’H (flavonoid3′-hydroxylase gene; Zhao et al, 2013 ), LFY ( LEAFY ; Ma et al, 2016 ), NAM ( No Apical Meristem ; Sha et al, 2017 ) and UEP1 (gene of ubiquitin extension protein; Annadana et al, 2002 ). Polymorphic regions mostly covering introns and 5′UTRs of five of the six genes were amplified using conserved primer pairs that were developed according to sequences acquired from GenBank ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Clarifying Recent Adaptive Diversification of the Chrysanthemum-Group on the Basis of an Updated Multilocus Phylogeny of Subtribe Artemisiinae (Asteraceae: Anthemideae)

et al. 2021

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Understanding the roles played by geography and ecology in driving species diversification and in the maintenance of species cohesion is the central objective of evolutionary and ecological studies. The multi-phased orogenesis of Qinghai-Tibetan Plateau (QTP) and global climate changes over late-Miocene has profoundly influenced the environments and evolution of organisms in this region and the vast areas of Asia. In this study, we investigate the lineage diversification of Chrysanthemum-group in subtribe Artemisiinae (tribe Anthemideae, Asteraceae) likely under the effects of climate changes during this period. Using DNA sequences of seven low-copy nuclear loci and nrITS and the coalescent analytical methods, a time-calibrated phylogeny of subtribe Artemisiinae was reconstructed with emphasis on Chrysanthemum-group. The monophyletic Chrysanthemum-group was well resolved into two major clades corresponding to Chrysanthemum and Ajania, two genera which can be well identified by capitulum morphology but have been intermingled in previous plastid and ITS trees. Within Chrysanthemum, a later divergence between Ch. indicum-complex and Ch. zawadskii-complex can be recognized. The time frames of these sequential divergences coincide with the late Cenozoic uplift of the Northern QTP and the concomitant climatic heterogeneity between eastern and inland Asia. Reconstruction of historical biogeography suggested the origin of Chrysanthemum-group in Central Asia, followed by eastward migration of Chrysanthemum and in situ diversification of Ajania. Within Chrysanthemum, Ch. indicum-complex and Ch. zawadskii-complex exhibited contemporary distributional division, the former in more southern and the latter in more northern regions. The geographic structure of the three lineages in Chrysanthemum-group have been associated with the niche differentiation, and environmental heterogenization in Asia interior.

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Cited by 29 publications

References 15 publications

Overexpression of SlOFP20 affects floral organ and pollen development

Overexpression of SlOFP20 affects floral organ and pollen development

The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development

Clarifying Recent Adaptive Diversification of the Chrysanthemum-Group on the Basis of an Updated Multilocus Phylogeny of Subtribe Artemisiinae (Asteraceae: Anthemideae)

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