We studied the isotope, pressure, and doping effects on the pseudogap temperature T * by neutron spectroscopic experiments of the relaxation rate of crystal-field excitations in La 1.96−x Sr x Ho 0.04 CuO 4 ͑x = 0.11, 0.15, 0.20, 0.25͒. We found clear evidence for the opening of a pseudogap at T * Ͼ T c in the whole doping range. Furthermore, the effect of oxygen isotope substitution was found to produce an upward shift of T * , whereas the application of pressure on the optimally doped compound ͑x = 0.15͒ results in a downward shift of T * . The opposite effect of oxygen isotope substitution and pressure application indicates that electronphonon induced effects contribute to the pseudogap formation. The temperature dependence of the relaxation data is consistent with a gap function of predominantly d-wave symmetry.