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Uchida, Akio; Nagamiya, Kenji; Takabe, Tetsuko

doi:10.1023/a:1025086505120

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…Regarding the Amaranthaceae family, the calli were derived from various explant types and were generally induced by a combination of NAA + BAP or BAP + IBA as for A. gmelini or S. pestifer , and the shoots emerged after their transfer to media with TDZ + NAA or BAP, respectively [ 53 , 66 ]. Moreover, callus induction occurred with 2,4-D only for B. sinuspercici and S. lanata , and with TDZ only for S. europaea , which illustrates the wide variety of possible treatments in this family [ 57 , 66 , 128 ].…”

Section: Micropropagation Of Halophyte Plantsmentioning

confidence: 99%

“…This was a pioneering work in the transformation of halophytic species and opened a door to transform other species as well as to extend our knowledge on the response to salinity in plants. Some years later, Uchida et al [ 53 ] carried out the transformation of A. gmelini callus with A. tumefaciens cells harboring the pBI121 plasmid. The transformed calluses were selected by GUS expression and histochemical assay, and the presence of the GUS gene was also confirmed by Southern blot.…”

Section: Genetic Transformation Studies (Transgenesis)mentioning

confidence: 99%

“…The transformed calluses were selected by GUS expression and histochemical assay, and the presence of the GUS gene was also confirmed by Southern blot. However, the transformation efficiency from calluses was very low (0.02%) [ 53 ]. Yuan et al [ 98 ] used shoot explants from L. bicolor for the transformation via A. tumefaciens harboring the plasmid pTCK303.…”

Section: Genetic Transformation Studies (Transgenesis)mentioning

confidence: 99%

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Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

Custódio

Charles

Magné

et al. 2022

Plants

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Halophytes are plants able to thrive in environments characterized by severe abiotic conditions, including high salinity and high light intensity, drought/flooding, and temperature fluctuations. Several species have ethnomedicinal uses, and some are currently explored as sources of food and cosmetic ingredients. Halophytes are considered important alternative cash crops to be used in sustainable saline production systems, due to their ability to grow in saline conditions where conventional glycophyte crops cannot, such as salt-affected soils and saline irrigation water. In vitro plant tissue culture (PTC) techniques have greatly contributed to industry and agriculture in the last century by exploiting the economic potential of several commercial crop plants. The application of PTC to selected halophyte species can thus contribute for developing innovative production systems and obtaining halophyte-based bioactive products. This work aimed to put together and review for the first time the most relevant information on the application of PTC to halophytes. Several protocols were established for the micropropagation of different species. Various explant types have been used as starting materials (e.g., basal shoots and nodes, cotyledons, epicotyls, inflorescence, internodal segments, leaves, roots, rhizomes, stems, shoot tips, or zygotic embryos), involving different micropropagation techniques (e.g., node culture, direct or indirect shoot neoformation, caulogenesis, somatic embryogenesis, rooting, acclimatization, germplasm conservation and cryopreservation, and callogenesis and cell suspension cultures). In vitro systems were also used to study physiological, biochemical, and molecular processes in halophytes, such as functional and salt-tolerance studies. Thus, the application of PTC to halophytes may be used to improve their controlled multiplication and the selection of desired traits for the in vitro production of plants enriched in nutritional and functional components, as well as for the study of their resistance to salt stress.

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Section: Micropropagation Of Halophyte Plantsmentioning

confidence: 99%

Section: Genetic Transformation Studies (Transgenesis)mentioning

confidence: 99%

Section: Genetic Transformation Studies (Transgenesis)mentioning

confidence: 99%

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Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

Custódio

Charles

Magné

et al. 2022

Plants

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In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide

et al. 2011

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To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (MS) salts and vitamins, supplemented with 5 mM phosphate, plus 1 mg L(-1) dichloropenoxy-acetic acid (2,4-D), and 87 mM sucrose (Stage 1 media). Only calli having RNS were able to regenerate plantlets. MS media plus phosphate was used throughout regeneration, with the Stage 2 media containing 2 mg L(-1) 6-benzylaminopurine, 43 mM sucrose and 1.5% soluble starch. Stage 3 media had no hormones or organic sources of carbon, and cultures were grown under ambient (~400 ppm) versus CO(2) enrichment (1.2% CO(2)). When calli without RNS were cultured under Stage 3 conditions with 1.2% CO(2), there was an increase in growth, protein content, and photosystem II yield, while structural and biochemical analyses indicated the cells in the calli had C(3) type photosynthesis. CO(2) enrichment during growth of RNS during Stage 3 had a large effect on regeneration success, increasing efficiency of shoot and root development, size of plantlets, leaf soluble protein, and chlorophyll concentration. Anatomical analysis of plantlets, which developed under 1.2% CO(2), showed leaves developed C(4) type chlorenchyma cells, including expression of key C(4) biochemical enzymes. Increasing salinity in the media, from 0 to 200 mM NaCl, increased tissue osmolality, average plantlet area and regeneration success, but did not affect protein or chlorophyll content.

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Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.

Tai

Jiang

et al. 2017

Plant Cell Rep

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A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation. The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD ranging from 1.0 to 1.5 and then selected with 25 mg/L hygromycin (Hyg). The transformed cells were identified from Hyg positive calli by GUS assay and qRT-PCR, and the transformation efficiency was up to 74.4%. The practicality of this system was further tested via genetic manipulation of S. europaea Na/H antiporter 1 (SeNHX1) gene by creating the overexpressing, silencing, and empty vector cells. Survival ratio and Na distribution under salt treatment showed obvious differences in SeNHX1-overexpressing, -silencing, and empty vector cells, indicating the feasibility of this system to analyze gene function. This investigation is enlightening for studies in other non-model plants lacking of self-dependent transformation system.

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Untitled

Cited by 11 publications

References 18 publications

Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review

In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide

Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.

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