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Ferenczy, Judith; Szegletes, T.; Bálint, T.; Ábrahám, Magdolna; Nemcsók, J.

doi:10.1023/a:1007701323808

Cited by 15 publications

(2 citation statements)

References 52 publications

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“…Following exposure, the whole brain was dissected out and placed in ice-cold 0.1-M sodium phosphate buffer (pH 8.0). In this study, brain sample was used as in teleost, AChE is maximally distributed in brain (Kopecka, Rybakowas, Barsiene, & Pempkowiak, 2004;Ferenczy, Szegletes, Balint, Abraham, & Nemcsok, 1997). Fish brains were then weighed and homogenized using a glass Teflon homogenizer in homogenization buffer (0.1 M sodium phosphate buffer, 0.1% Triton X-100, and pH 8.0) to achieve a final concentration of 20 mg tissue/ml phosphate buffer.…”

Section: Measurement Of the Ache Activitymentioning

confidence: 99%

Toxic effects of an organophosphate pesticide, envoy 50 SC on the histopathological, hematological, and brain acetylcholinesterase activities in stinging catfish (Heteropneustes fossilis)

Akter

Pervin

Jahan

et al. 2020

JoBAZ

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Background: Freshwater fish in Bangladesh are adversely affected by the washed off pesticides, used in agriculture. The aim of this study was to evaluate the impacts of a commonly used organophosphate pesticide on freshwater stinging catfish, Heteropneustes fossilis, which envisioned that the possible threats might occur by this organophosphate group to other species in the wild. Methodology: To study the potential hazards of Envoy 50 SC on H. fossilis, fry of the fish were exposed to the acute toxicity tests. Changes in the hematological parameters, organ-specific histomorphologies, and brain acetylcholinesterase (AChE) activities were determined by treating the fish with agricultural recommended dose and below that dose of 0.015 and 0.0075 ppm, respectively. Results: LC 50 of Envoy 50 SC for the fish was determined as 0.151 (0.014-0.198) ppm. Pesticide abruptly altered the normal tissue structures of the gill, liver, and kidney. The major alterations included were the gill lamellae missing, gill clubbing, hyperplasia, nuclear hypertrophy, vacuolation, glomerular expansion, increasing diameter of the renal tubules, hemorrhage, necrosis, and pyknosis. In blood cells, changes observed in the peripheral nuclear erythrocyte were large lymphocyte, dead cell, fusion of the cells, binucleated cells, tear-shaped cells, ghost cells, senile cells, and abnormal structures of the cells. Significantly lower (P < 0.05) red blood cell (RBC) count and AChE activities in fish brain due to the pesticide exposure suggested the reasons of abrupt behavior, increased oxygen consumption, and fish mortality at higher concentration of this organophosphate pesticide. Conclusion: The presence of pesticides, even at low concentrations, caused deleterious effects on the earlier life stages of a comparatively harder and robust fish, suggesting a wider range effect on the more sensitive wild life, in particular decrease in survival in their native environment. Therefore, measures should be taken to minimize the risk of contamination of the aquatic environment by such toxic chemicals.

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Section: Measurement Of the Ache Activitymentioning

confidence: 99%

Toxic effects of an organophosphate pesticide, envoy 50 SC on the histopathological, hematological, and brain acetylcholinesterase activities in stinging catfish (Heteropneustes fossilis)

Akter

Pervin

Jahan

et al. 2020

JoBAZ

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“…Following exposure, three fishes were taken from each aquarium (n=9) for each pesticide. As AChE is maximally distributed in the brain of teleost (Kopecka, Rybakowas, Baršiene, & Pempkowiak, 2004;Ferenczy, Szegletes, Bálint, Ábrahám, & Nemcsók, 1997), brain samples were used to measure AChE activity. Brains were dissected and were placed in ice-cold 0.1-M sodium phosphate buffer (pH 8.0).…”

Section: Ache Activity Measurement Of Ops Treated Fishmentioning

confidence: 99%

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Reza¹,

Rakhi²,

Hossen³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Agricultural pesticides, eventually find their ways to aquatic ecosystems by different routes adversely affect the aquatic biota. To determine its potential hazards, acute toxicity tests (LC50) of three commonly used organophosphate pesticides, Envoy 50SC, Samcup 50EC and Dursban 20EC on Labeo rohita and Barbonymus gonionotus were performed. The LC50 (P<0.05) of these pesticides were estimated at 0.110 (0.060-0.199), 0.217 (0.204-0.231) and 0.079 (0.073-0.084) ppm for L. rohita and 0.471 (0.440-0.500), 0.789 (0.754-0.824) and 0.273 (0.260-0.286) ppm for B. gonionotus, respectively. Pesticides abruptly altered the normal structures in various fish organs like gills, kidney and liver. The major alterations included missing gill lamellae, gill clubbing, fungal granuloma, fatty degeneration, lipid droplet formation, degenerating glomeruli and kidney tubules, hyperplasia, hemorrhage, pyknosis, increased number of vacuoles, and necrosis. Envoy 50SC, Samcup 20EC and Dursban 20EC showed significant inhibition on L. rohita AChE activity at 216.7±11.0, 207.3±5.0 and 146.7±5.5 nmol/min/mg protein, respectively. In B. gonionotus, Samcup 20EC and Dursban 20EC showed significant inhibition (P<0.05), which were recorded as 242.0±6.6 and 221.7±60.3 nmol/min/mg protein, respectively. Furthermore, pesticide treated L. rohita showed higher enzymatic inhibition (51.49%) than B. gonionotus (19.60%).

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