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Bataillon, Michel; Cardinali, Angela; Duchiron, Francis

doi:10.1023/a:1005415207188

Cited by 40 publications

(9 citation statements)

References 13 publications

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“…However, it was higher than 5.5 kDa as observed by Bastawde (4). In contrast, some workers documented a higher molecular weight than that observed in the present study (5,19,20,38,39). Low molecular weight xylanases are preferred for commercial application in paper and pulp industry as they penetrate throughout the pulp fibres more effectively to hydrolyze xylan making the fiber structure more permeable to extraction of lignin by chemicals (21).…”

Section: Molecular Weight Determinationcontrasting

confidence: 54%

“…Further, recovery of the purified enzyme from B. subtilis ASH following one step protocol was better than that obtained by using multistep protocol (1,5,16,24). However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7).…”

Section: Purification Of Xylanasementioning

confidence: 96%

“…Other research groups reported purification of xylanase by employing a combination of two or more different methods including salt fractionation, ion-exchange, gel filtration and hydrophobic interaction chromatography (1,5,7,16,24,29,35,36). The overall purification in the present study using one step was higher than that reported from Bacillus amyloliquefaciens (7) and Bacillus circulans (36) using a multistep sequence of purification.…”

Section: Purification Of Xylanasementioning

confidence: 99%

“…However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7). …”

Section: Purification Of Xylanasementioning

confidence: 99%

See 3 more Smart Citations

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH.Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 °C. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton kinetics towards birch wood xylan with apparent K m 3.33 mg/ml and V max 100 IU/ml. The enzyme was strongly inhibited by Hg 2+ and Cu 2+ while enhanced by Co 2+ and Mn 2+. The purified enzyme could be stored at 4 ºC for six weeks without any loss of catalytic activity. The faster and economical purification of the cellulase-free xylanase from B. subtilis ASH by onestep procedure together with its appreciable stability at high temperature and alkaline pH makes it potentially effective for industrial applications.

show abstract

Section: Molecular Weight Determinationcontrasting

confidence: 54%

Section: Purification Of Xylanasementioning

confidence: 96%

Section: Purification Of Xylanasementioning

confidence: 99%

“…However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7). …”

Section: Purification Of Xylanasementioning

confidence: 99%

See 2 more Smart Citations

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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“…Thermostable xylanases active at alkaline pH are of great importance for application in pulp and paper industry to decrease the consumption of chlorine chemicals [17]. There are reports of isolation of xylan degrading alkali tolerant thermophiles from the hot springs in Bulgaria [12] and Portugal [5]. Bacteria belonging to the genus Bacillus have been largely reported to produce cellulase free xylanases [6,30].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular characterization of thermo-alkali tolerant xylanase producing bacteria from thermal springs of Manikaran

et al. 2010

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One hundred ten alkalo-tolerant thermophilic bacteria were isolated from 17 samples (water and sediment) collected from Manikaran. Of 110 isolates, 70 showed the production of xylanases and were further screened for growth and production of xylanases at different temperature ranging from 40 to 75°C. Eleven isolates that showed growth and xylanase production at temperatures ≥50°C were selected for quantitative estimation in modified Reese mineral liquid medium containing wheat bran. Maximum xylanase activity was produced by isolate H-7 followed by H-9 and R-9 and was statistically superior to other isolates. The microscopic observation showed that the isolates possessed the typical rod with endospore, characteristic of genus Bacillus. The isolates were found to be oxidase and catalase positive. Using BIOLOG Microlog 3 software, the isolates H7, H9 and R9 were identified as Paenibacillus ehemensis, Bacillus cereus/B. thuringiensis and B. subtilis respectively, based on utilization of 95 carbon sources. PCR-RFLP analysis of 16S rDNA indicated that the isolates were genetically different from each other. DNA sequencing of the three isolates and phylogenetic analysis revealed that all the isolates obtained from Manikaran thermal springs showed 97 to 100% similarity with the sequences within the GenBank. The closest phylogenetic neighbours according to the 16S rRNA gene sequence data for the three isolates H-7, H-9, and R-9 were Paenibacillus ehemensis, Bacillus cereus and Bacillus subtilis, respectively.

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Effect of Bacillus circulans D1 Thermostable Xylanase on Biobleaching of Eucalyptus Kraft Pulp

Bocchini

Damiano

Gomes

et al. 2003

Biotechnology for Fuels and Chemicals

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Cited by 40 publications

References 13 publications

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Biochemical and molecular characterization of thermo-alkali tolerant xylanase producing bacteria from thermal springs of Manikaran

Effect of Bacillus circulans D1 Thermostable Xylanase on Biobleaching of Eucalyptus Kraft Pulp

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