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Goka, Kouichi

doi:10.1023/a:1006062807613

Cited by 51 publications

(1 citation statement)

References 19 publications

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“…This mite has developed a high degree of resistance to various acaricides (Aiki et al 2005; Goka 1998; Kuwahara 1982, 1984; Kuwahara et al 1982; Mizutani et al 1988; Osakabe 1968), so it has become increasingly important to find ways to control the species using natural enemies. The phytoseiid mite Neoseiulus womersleyi (Schicha) (Acari: Phytoseiidae) is one of the most important predators of the Kanzawa spider mite (Hamamura 1986), and some N. womersleyi strains resistant to pesticides have been found (Hamamura 1986; Mochizuki 1990, 1994).…”

Section: Introductionmentioning

confidence: 99%

Population structure of the predatory mite Neoseiulus womersleyi in a tea field based on an analysis of microsatellite DNA markers

2010

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The predatory mite Neoseiulus womersleyi (Schicha) (Acari: Phytoseiidae) is an important natural enemy of the Kanzawa spider mite, Tetranychus kanzawaki Kishida (Acari: Tetranychidae), in tea fields. Attraction and preservation of natural enemies by habitat management to reduce the need for acaricide sprays is thought to enhance the activity of N. womersleyi. To better conserve N. womersleyi in the field, however, it is essential to elucidate the population genetic structure of this species. To this end, we developed ten microsatellite DNA markers for N. womersleyi. We then evaluated population structure of N. womersleyi collected from a tea field, where Mexican sunflower, Tithonia rotundifolia (Mill.), was planted to preserve N. womersleyi. Seventy-seven adult females were collected from four sites within 200 m. The fixation indexes FST among subpopulations were not significantly different. The kinship coefficients between individuals did not differ significantly within a site as a function of the sampling dates, but the coefficients gradually decreased with increasing distance. Bayesian clustering analysis revealed that the population consisted of three genetic clusters, and that subpopulations within 100 m, including those collected on T. rotundifolia, were genetically similar to each other. Given the previously observed population dynamics of N. womersleyi, it appears that the area inhabited by a given cluster of the mite did not exceed 100 m. The estimation of population structure using microsatellite markers will provide valuable information in conservation biological control.

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