149. The Role of the Lymphatic System in the Absorption of Micrurus fulvius Venom

Paniagua, Dayanira; Jiménez, Luis Felipe; Núñez, Camilo Romero; Vergara, Irene; Calderón, Alma Graciela García; Benard, Melisa; Bernas, Michael; Sevcik, Carlos; Witte, Marlys H.; Boyer, Leslie V.; Alagón, Alejandro

doi:10.1016/j.toxicon.2012.04.150

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that toxins can be absorbed through the lymphatics (36). Toxins absorbed in the lymph pool act as a depository providing sustained release of toxin back into the blood stream yielding a complex absorption process (37).…”

Section: Discussionmentioning

confidence: 99%

Time-Resolved MRI Assessment of Convection-Enhanced Delivery by Targeted and Nontargeted Nanoparticles in a Human Glioblastoma Mouse Model

et al. 2019

View full text Add to dashboard Cite

Convection-enhanced delivery (CED) provides direct access of infusates to brain tumors, however, clinical translation of this technology has not been realized because of the inability to accurately visualize infusates in real-time and lack of targeting modalities against diffuse cancer cells. In this study we use time-resolved MRI to reveal the kinetics of CED processes in a glioblastoma (GBM) model using iron oxide nanoparticles (NPs) modified with a glioma targeting ligand, chlorotoxin (CTX). Mice bearing orthotopic human GBM tumors were administered a single dose of targeted CTX-conjugated NP (NPCP-CTX) or non-targeted NP (NPCP) via CED. High resolution T2weighted, T2*-weighted and quantitative T2 MRI was utilized to image NP delivery in real-time and determine the volume of distribution (VD) of NPs at multiple time points over the first 48 h post-CED. GBM specific targeting was evaluated by flow cytometry and intracellular NP localization by histological assessment. NPCP-CTX produced a V D of 121 ± 39 mm 3 at 24 h, a significant increase compared to NPCP, while exhibiting GBM specificity and localization to cell nuclei. Notably, CED of NPCP-CTX resulted in a sustained expansion of VD well after infusion, suggesting a possible active transport mechanism which was further supported by the presence of NPs in endothelial and red blood cells. In summary, we show that time-resolved MRI is a suitable modality to study CED kinetics and CTX-mediated CED facilitates extensive distribution of infusate and specific targeting of tumor cells.

show abstract

Section: Discussionmentioning

confidence: 99%