[145] Plant tyrosinase (polyphenol oxidase)

Dawson, Charles R.; Magee, Richard J.

doi:10.1016/s0076-6879(55)02309-4

Cited by 53 publications

(24 citation statements)

References 9 publications

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“…Em 1896 foi atribuído o nome de tirosinase a enzima presente no cogumelo, sendo assim chamada porque o primeiro substrato usado foi a tirosina [97][98][99][100][101][102][103][104][105][106][107] .…”

Section: Compostos Fenólicosunclassified

“…6) como difenóis (e.g. catecol, L-dopa, dopamina, adrenalina) (Eq.7) [97][98][99][100][101][102][103][104][105][106][107] . É largamente distribuída na natureza e encontrada nos tecidos de kiwi, arroz, abacate, pêra, maçã, morango, uva, folha de espinafre, palmito, banana, batata inglesa, batata doce, pêssego, manga, berinjela, inhame entre outros [47][48][49][50][108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124][125][126] .…”

Section: Compostos Fenólicosunclassified

“…Este escurecimento é causado pela ação dessa enzima com os compostos fenólicos naturais e o oxigênio atmosférico que são oxidados a o-quinona, que em seguida pode se polimerizar e formar as melaninas [101][102][103][104] . Nos tecidos vegetais, a enzima PFO esta localizada nos cloroplastos 103,104 e sua concentração (atividade) no tecido vegetal depende do local do plantio, período da colheita, espécie e do estado de amadurecimento deste, sendo menor em frutos ou vegetais não-amadurecidos 103,104 .…”

Section: Compostos Fenólicosunclassified

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Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Fatibello‐Filho¹,

Vieira²

2002

Quím. Nova

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Section: Compostos Fenólicosunclassified

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Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Fatibello‐Filho¹,

Vieira²

2002

Quím. Nova

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“…A total of 9 sub-samples were thus obtained from one single banana slice. After the cutting operation every part of the banana slice was subjected to the following measurements: -residual moisture content by thermo-gravimetric technique (results expressed on dry weight basis, g H 2 O /g DS ); -reducing sugars content by the DSN colorimetric method (Miller 1959) (results expressed as grams of reducing sugar per mass of dried solid, g sugar /g DS ); -total polyphenols content by Folin-Ciocalteau determination (Singleton et al 1999) (results expressed as milligrams of gallic acid equivalent per mass of dried solid, mg/g DS ); -poly-phenol-oxidase activity (tyrosine activity) by spectrophotometric technique (Dawson & Magee 1955) (results expressed as units -that derives from enzymatic activity -per mass of dried solid, mg/g DS ).…”

Section: Methodsmentioning

confidence: 99%

Microwave assisted drying of banana: effects on reducing sugars and polyphenols contents

Barba¹,

D’Amore²,

Rispoli³

et al. 2014

Czech J. Food Sci.

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Barba A.A., d'Amore M., Rispol M., Marra F., Lamberti G. (2014): Microwave assisted drying of banana: effects on reducing sugars and polyphenols contents. Czech J. Food Sci., 32: 369-375.The effects of microwave assisted drying on banana fruit was evaluated. Water, reducing sugars, and polyphenol contents, as well as poly-phenol-oxidase activity were evaluated along the radial and axial positions in thick slices of banana, according to a properly defined cutting and assaying protocol. The effects of the microwave-assisted drying process were compared to the convective air-assisted drying resulting faster than the conventional process. In particular, the resulting samples were homogeneous in the water content; the contents of reducing sugars were strongly decreased on drying with microwaves; the poly-phenol-oxidase was inactivated by the high temperature produced by the process and thus the polyphenols content remained practically the same as in the fresh product.

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“…Antioxidants are commonly used to deal with problems related to phenolics. Examples include 2 β-mercaptoethanol, ascorbic acid, Bovine Serum Albumin, sodium azide and PVP amongst others (Dawson and Magee, 1995;Clark, 1997).…”

Section: Introductionmentioning

confidence: 99%

A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

Puchooa¹

2004

Afr. J. Biotechnol.

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A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.

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[145] Plant tyrosinase (polyphenol oxidase)

Cited by 53 publications

References 9 publications

Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Microwave assisted drying of banana: effects on reducing sugars and polyphenols contents

A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

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