143. Studies on phosphorylation. Part XXII. Phosphorylation accompanying the oxidation of quinol phosphates

Mechanisms of Nucleophilic Substitution in Phosphate Esters

“…It is tempting to formulate the first two of these sequences (Eq. 19 and 20) as producing monometaphosphate anion, and evidence consistent with this view has been presented (61).…”

Oxidative Phosphorylation in Mitochondria

“…The model studies of Wang and co-workers (283)(284)(285)304) are attrac- (257) Pi addition to pyridlne :ring of (263) NADH (260)(261)(262) Oxidation of quinol pho:~phates (264,265) Pi addition to 4 position of quinone methines q-oxldation (271) Addition of Pi or thiol II to carbonyl of a quinone-loxidation (272) Pyrophosphates formed (268)(269)(270) in the reaction (265)(266)(267) Pi addition to = CH2 ot quinone methine.q-l-oxidation (273, 274) (275,276) Addition of Pi to quinone methine complexed with non-berne Fe, followed by oxidation (277) Chemi-osmotic coupling (169 Phosphoimidazolc (286,287) …”

The mitochondrial oxidation of quinol monophosphates

“…150°C (decomp.). This product was stable on exposure to air, but impure samples turned pink under these conditions, as described by Clark et al (1961). Attempts to prepare a stable sample of menadiol 4-phosphate failed, even though the best sample had m.p.…”

“…Quinol monophosphates have received considerable attention as possible intermediates in oxidative phosphorylation after the demonstration that under oxidative conditions they function as phosphoryl transfer agents (Clark, Kirby & Todd, 1958;Wieland & Pattermann, 1958Clark, Hutchinson, Kirby & Todd, 1961). The particularly attractive feature of these compounds is their obvious relationship to components of the electrontransport chain of both mammalian and bacterial systems and several theoretical schemes for oxidative phosphorylation incorporating ubiquinol monophosphate or a derivative have appeared in the literature (Chmielewska, 1960;Vilkas & Lederer, 1962;Williams, 1962;Erickson, Wagner & Folkers, 1963).…”