14 Mating-Type Structure, Function, Regulation and Evolution in the Pezizomycotina

Dyer, Paul S.; Inderbitzin, Patrik; Debuchy, Robert

doi:10.1007/978-3-319-25844-7_14

Cited by 52 publications

(84 citation statements)

References 214 publications

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“…Later work showed that the mating factors actually represent large chromosomal sections conferring mating specificity (Bistis, 1998;Lindegren, 1936b). Also, the two "versions" of these chromosomal sections are markedly different as they are maintained through the suppression of recombination between them (Dyer et al, 2016) and the genes they encode are highly divergent (Butler, 2007;Debuchy et al, 2010). Because the "versions" of the MAT1 locus do not conform to what is generally expected for true alleles (i.e.…”

Section: Hyp Locusmentioning

confidence: 99%

“…individuals carrying either the MAT1-1 or MAT1-2 idiomorph) typically display substantial similarity outside the locus (including the regions immediately flanking it) and an overall lack of sequence similarity in the idiomorphic regions themselves McGuire et al, 2001;Turgeon, 1998). Within this region of dissimilarity, the MAT1-1 idiomorph generally encodes one to three genes, while the MAT1-2 idiomorph often encode a single gene (Butler, 2007;Dyer et al, 2016).…”

Section: Hyp Locusmentioning

confidence: 99%

“…Comparisons of the MAT1 locus across the Pezizomycotina has revealed a high level of positional conservation as it is usually located at similar chromosomal positions (Butler, 2007;Debuchy and Turgeon, 2006;Dyer et al, 2016;Turgeon and Yoder, 2000). The genes immediately flanking the MAT1-1 and MAT1-2 idiomorphs typically include SLA2 that encodes the cytoskeleton assembly control protein and APN2 that encodes AP endonuclease.…”

Section: Hyp Locusmentioning

confidence: 99%

“…Although very elegant, the application of these rules has in some cases proved difficult, often resulting in inconsistent and ambiguous assignment of gene names. Such discrepancies represent significant barriers to meaningful discussions on the origin, functioning and evolutionary history of the genes underlying sexual reproduction in fungi Debuchy and Turgeon, 2006;Dyer et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered

Wilken

Steenkamp

Wingfield

et al. 2017

Fungal Biology Reviews

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Section: Hyp Locusmentioning

confidence: 99%

Section: Hyp Locusmentioning

confidence: 99%

Section: Hyp Locusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered

Wilken

Steenkamp

Wingfield

et al. 2017

Fungal Biology Reviews

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“…The presence of either of two distinct alleles of the MAT locus in each B. bassiana isolate indicates that the species is putatively heterothallic. A new MAT gene was detected next to the MAT 1–2–1 gene which, based on homology to a newly described gene in Villosiclava virens [52], is referred to as MAT 1–2–8 following the nomenclature proposed by Dyer et al [66]. We attempted to sexually cross different isolates of B. bassiana in several combinations to generate fertile fruiting bodies.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

et al. 2016

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BackgroundEntomopathogenic fungi such as Beauveria bassiana are promising biological agents for control of malaria mosquitoes. Indeed, infection with B. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. Natural isolates of B. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. In this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three RNA libraries, and applied a genome comparison approach to uncover genetic mechanisms underpinning virulence.ResultsA high-quality, near-complete genome assembly was achieved for the highly virulent isolate Bb8028, which was compared to the assemblies of the four other isolates. Whole genome analysis showed a high level of genetic diversity between the five isolates (2.85–16.8 SNPs/kb), which grouped into two distinct phylogenetic clusters. Mating type gene analysis revealed the presence of either the MAT1–1–1 or the MAT1–2–1 gene. Moreover, a putative new MAT gene (MAT1-2–8) was detected in the MAT1–2 locus. Comparative genome analysis revealed that Bb8028 contains 163 genes exclusive for this isolate. These unique genes have a tendency to cluster in the genome and to be often located near the telomeres. Among the genes unique to Bb8028 are a Non-Ribosomal Peptide Synthetase (NRPS) secondary metabolite gene cluster, a polyketide synthase (PKS) gene, and five genes with homology to bacterial toxins. A survey of candidate virulence genes for B. bassiana is presented.ConclusionsOur results indicate several genes and molecular processes that may underpin virulence towards mosquitoes. Thus, the genome sequences of five isolates of B. bassiana provide a better understanding of the natural variation in virulence and will offer a major resource for future research on this important biological control agent.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3339-1) contains supplementary material, which is available to authorized users.

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Culturing and Mating of Aspergillus fumigatus

Ashton

Dyer

2019

CP Microbiology

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Aspergillus fumigatus is an opportunistic human fungal pathogen, capable of causing invasive aspergillosis in patients with compromised immune systems. The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in 2009, with methods described to induce mating under laboratory conditions. The presence of a sexual cycle now offers a valuable tool for classical genetic analysis of the fungus, such as allowing determination of whether traits of interest are mono‐ or poly‐genic in nature. For example, the sexual cycle is currently being exploited to determine the genetic basis of traits of medical importance such as resistance to azole antifungals and virulence, and to characterize the genes involved. The sexual cycle can also be used to assess the possibility of gene flow between isolates. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. This unit describes protocols for culturing of A. fumigatus and for inducing sexual reproduction between compatible MAT1‐1 and MAT1‐2 isolates of the species. The unit also provides working methods for harvesting sexual structures, isolating single‐spore progeny and confirming whether sexual recombination has occurred. © The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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14 Mating-Type Structure, Function, Regulation and Evolution in the Pezizomycotina

Cited by 52 publications

References 214 publications

Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered

Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered

Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

Culturing and Mating of Aspergillus fumigatus

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