14-3-3γ regulates Copine1-mediated neuronal differentiation in HiB5 hippocampal progenitor cells

Yoo, Jae Cheal; Park, Nammi; Lee, Boah; Nashed, Abdullateef; Kim, So Yeon; Lee, Da Yong; Kim, Ajung; Hwang, Eun Mi; Yi, Gwan Su; Park, Jae Yong

doi:10.1016/j.yexcr.2017.04.015

Cited by 8 publications

(8 citation statements)

References 22 publications

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“…Meanwhile, the upregulation of TNF-α influenced the expression of CPNE1 and a positive feedback mechanism existed between CPNE1 and TNF-α. Also, CPNE1 mediated NF-kB signaling by facilitating TNF-α-dependent Inhibitory-κB (IkB) degradation [ 40 , 41 ]. Via interacting directly with p65, CPNE1 lead to shear of p65 N-terminus and terminated the transcription of NF-kB, which in turn inhibited transcriptional activation of NF-kB [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma

Huang

Wang

et al. 2022

Cancer Cell Int

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Background Copine1 (CPNE1), the first discovered CPNE1 family member, participates in the process of carcinogenesis and development of diverse tumors. Our study aimed to investigate the expression and prognostic value of CPNE1 gene in hepatocellular carcinoma (HCC), to explore its functional network in HCC and its effects on biological behaviors. Methods HCCDB, CCLE, HPA and LinkedOmics online databases were used to explore the expression of CPNE1 gene and analyze the co-expression network of CPNE1 in hepatocellular carcinoma. Gene set enrichment analysis (GSEA) was used for GO functional annotation, KEGG pathway enrichment analysis and regulators of CPNE1 networks in LIHC. HepG2 and MHCC-97H cells were selected to construct CPNE1 knockdown cell lines by transfection with siRNA, and Hep3B cell was selected to construct CPNE1 overexpression cell line by transfection with plasmid. The effect of CPNE1 on the proliferation of hepatocellular carcinoma cells was examined by CCK8 assay and clone formation assay; the effect of CPNE1 on the migration ability of hepatocellular carcinoma cells was assessed by cell scratch assay and Transwell cell migration assay; finally, the expression of related signaling pathway proteins was examined by Western Blot. The correlation of CPNE1 expression with immune infiltration and immune checkpoint molecules in HCC tissues was analyzed using TIMER online database and GSEA. Results CPNE1 was highly expressed in HCC tissues and significantly correlated with sex, age, cancer stage and tumor grade. Overall survival (OS) was significantly lower in patients with high CPNE1 expression than in patients with low CPNE1 expression, and CPNE1 could be used as an independent prognostic indicator for HCC. Knockdown of CPNE1 gene inhibited the AKT/P53 pathway, resulting in decreased proliferation, migration and invasion of HCC cells. Overexpression of CPNE1 gene showed the opposite results. The level of CPNE1 expression in HCC was significantly and positively correlated with the level of infiltration of B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells (P < 0.001). GSEA results also showed that CPNE1 of LIHC was involved in some immune response regulating signaling pathways. Conclusions Our study firstly found the expression of CPNE1 was significantly higher in LIHC tissues than in normal liver tissues, and high CPNE1 expression was associated with poor prognosis. In addition, we identified the possible mechanism by which CPNE1 functioned in LIHC. CPNE1 influenced AKT/P53 pathway activation and LIHC cell proliferation and migration. There was a significant correlation between CPNE1 expression and tumor immune infiltration in LIHC.

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Section: Discussionmentioning

confidence: 99%

CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma

Huang

Wang

et al. 2022

Cancer Cell Int

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“…This is achieved by regulating the catenin/Rho GTPase/Limk1/cofilin signaling pathway to promote F-actin formation (47). 14-3-3ɣ regulates Cpne1 to enhance differentiation of hippocampal progenitor cells and neurite formation in these cells (21,48). Map1b plays an important role in axon guidance and axonal branching during the development of the nervous system (49,50).…”

Section: Discussionmentioning

confidence: 99%

Molecular studies into Copine-4 function in Retinal Ganglion Cells

Goel

Aponte

Wistow

et al. 2021

Preprint

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The molecular mechanisms underlying morphological diversity in retinal cell types are poorly understood. We have previously reported that several members of the Copine family of Ca-dependent membrane adaptors are expressed in Retinal Ganglion Cells (RGCs) and transcriptionally regulated by Brn3 transcription factors. Several Copines are enriched in the retina and their over-expression leads to morphological changes reminiscent of neurite formation in HEK293 cells. However, the role of Copines in the retina is largely unknown. Here we focus on Cpne4, a Copine whose expression is restricted to RGCs. Over-expression of Cpne4 in RGCs in vivo led to formation of large varicosities on the dendrites but did not otherwise visibly affect dendrite or axon formation. Protein interactions studies using yeast two hybrid analysis from whole retina cDNA revealed two Cpne4 interacting proteins - HCFC1 and Morn2. Mass Spectrometry analysis of retina lysate pulled down using Cpne4 or its vonWillebrand A (vWA) domain identified a further 207 interacting proteins. Gene Ontology (GO) analysis of Cpne4 interactors suggests its involvement in several metabolic and signaling pathways, including processes related to vesicle trafficking, intracellular membrane bound organelles, and plasma membrane associated structures, including neurites. We conclude that, consistent with its domain structure, Cpne4 may be involved in assembly and trafficking of several membrane associated cell compartments.

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“…This is achieved by regulating the catenin/Rho GTPase/Limk1/cofilin signaling pathway to promote F-actin formation [ 59 ]. 14-3-3ɣ regulates Cpne1 to enhance differentiation of hippocampal progenitor cells and neurite formation in these cells [ 29 , 60 ]. Map1b plays an important role in axon guidance and axonal branching during the development of the nervous system [ 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular studies into cell biological role of Copine-4 in Retinal Ganglion Cells

et al. 2021

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The molecular mechanisms underlying morphological diversity in retinal cell types are poorly understood. We have previously reported that several members of the Copine family of Ca-dependent membrane adaptors are expressed in Retinal Ganglion Cells and transcriptionally regulated by Brn3 transcription factors. Several Copines are enriched in the retina and their over-expression leads to morphological changes -formation of elongated processes-, reminiscent of neurites, in HEK293 cells. However, the role of Copines in the retina is largely unknown. We now investigate Cpne4, a Copine whose expression is restricted to Retinal Ganglion Cells. Over-expression of Cpne4 in RGCs in vivo led to formation of large varicosities on the dendrites but did not otherwise visibly affect dendrite or axon formation. Protein interactions studies using yeast two hybrid analysis from whole retina cDNA revealed two Cpne4 interacting proteins–Host Cell Factor 1 and Morn2. Mass Spectrometry analysis of retina lysate pulled down using Cpne4 or its vonWillebrand A domain showed 207 interacting proteins. A Gene Ontology analysis of the discovered proteins suggests that Cpne4 is involved in several metabolic and signaling pathways in the retina.

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14-3-3γ regulates Copine1-mediated neuronal differentiation in HiB5 hippocampal progenitor cells

Cited by 8 publications

References 22 publications

CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma

CPNE1 is a potential prognostic biomarker, associated with immune infiltrates and promotes progression of hepatocellular carcinoma

Molecular studies into Copine-4 function in Retinal Ganglion Cells

Molecular studies into cell biological role of Copine-4 in Retinal Ganglion Cells

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