14-3-3 Proteins SGF14c and SGF14l Play Critical Roles during Soybean Nodulation

Radwan, Osman; Wu, Xia; Govindarajulu, Manjula; Libault, Marc; Neece, David J.; Oh, Man‐Ho; Berg, R. Howard; Stacey, Gary; Taylor, Christopher G.; Clough, Steven J.

doi:10.1104/pp.112.207027

Cited by 32 publications

(26 citation statements)

References 52 publications

(76 reference statements)

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“…Li and Dhaubhadel [61] identified 18 genes (SGF 14a-r) coding 14-3-3 proteins in the whole soybean genome. Previous studies revealed that two of them (SGF14c and SGF14l) play critical roles in RNS [62] and homologs of SGF14b in L. japonicus were located in the peribacteroid membrane [63]. In our study, we found another two 14-3-3 proteins, Glyma.14G176900 (SGF14k) and Glyma.02G208700 (SGF14g), which are hubs that interacted with B. diazoefficiens to a high degree (Table 3).…”

Section: Resultssupporting

confidence: 56%

Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis

Zhang

Liu

et al. 2018

Preprint

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Although the legume-rhizobium symbiosis is a most important biological process, there is a limited knowledge about the protein interaction network between host and symbiont. Using interolog and domain-based approaches, we constructed an inter-species protein interactome with 5115 protein-protein interactions between 2291 Glycine max and 290 Bradyrhizobium diazoefficiens USDA 110 proteins. The interactome was validated by expression pattern analysis in nodules, GO term semantic similarity, and co-expression analysis. One sub-network was further confirmed using luciferase complementation image assay. In the G. max-B. diazoefficiens interactome, bacterial proteins are mainly ion channel and transporters of carbohydrates and cations, while G. max proteins are mainly involved in the processes of metabolism, signal transduction, and transport. We also identified the top ten highly interacting proteins (hubs) for each of the two species. KEGG pathway analysis for each hub showed that two 14-3-3 proteins (SGF14g and SGF14k) and five heat shock proteins in G. max are possibly involved in symbiosis, and ten hubs in B. diazoefficiens may be important symbiotic effectors. Subnetwork analysis showed that 18 symbiosis-related SNARE proteins may play roles in regulating bacterial ion channels, and SGF14g and SGF14k possibly regulate the rhizobium dicarboxylate transport protein DctA. The predicted interactome and symbiosis proteins provide a valuable basis for understanding the molecular mechanism of root nodule symbiosis in soybean.

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Section: Resultssupporting

confidence: 56%

Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis

Zhang

Liu

et al. 2018

Preprint

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“…It has been proposed that the lipid secondary messengers directly affect the calcium channels present at the nuclear membrane, resulting in the activation of CCaMK (Delmas et al, 2005;Oldroyd and Downie, 2006;Downie, 2014). Additional signaling proteins that have been shown to affect nodule formation include members of the mitogen-activated protein kinase cascade, 14-3-3 proteins, monomeric GTPases of Rab and Rac family, and the heterotrimeric GTP binding proteins (FernandezPascual et al, 2006;Blanco et al, 2009;Chen et al, 2012;Ke et al, 2012;Radwan et al, 2012;. Of these, the components of heterotrimeric G-protein complex are especially interesting as these are traditionally known to interact with the receptors at the plasma membrane and relay the information to intracellular targets in a wide range of signaling pathways in all eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

Choudhury

Pandey

2015

Plant Cell

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ORCID IDs: 0000-0002-6738-3574 (S.R.C.); 0000-0002-5570-3120 (S.P.)Signaling pathways mediated by heterotrimeric G-protein complexes comprising Ga, Gb, and Gg subunits and their regulatory RGS (Regulator of G-protein Signaling) protein are conserved in all eukaryotes. We have shown that the specific Gb and Gg proteins of a soybean (Glycine max) heterotrimeric G-protein complex are involved in regulation of nodulation. We now demonstrate the role of Nod factor receptor 1 (NFR1)-mediated phosphorylation in regulation of the G-protein cycle during nodulation in soybean. We also show that during nodulation, the G-protein cycle is regulated by the activity of RGS proteins. Lower or higher expression of RGS proteins results in fewer or more nodules, respectively. NFR1 interacts with RGS proteins and phosphorylates them. Analysis of phosphorylated RGS protein identifies specific amino acids that, when phosphorylated, result in significantly higher GTPase accelerating activity. These data point to phosphorylation-based regulation of G-protein signaling during nodule development. We propose that active NFR1 receptors phosphorylate and activate RGS proteins, which help maintain the Ga proteins in their inactive, trimeric conformation, resulting in successful nodule development. Alternatively, RGS proteins might also have a direct role in regulating nodulation because overexpression of their phospho-mimic version leads to partial restoration of nodule formation in nod49 mutants.

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“…In EAR, a predicted dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial-like (spot 4606), S-adenosyl-L-homocysteine hydrolase (spot 6501), urease (spot 5805), putative V-type proton ATPase subunit B 1-like (spot 2605), a predicted fructokinase-2-like (spot 2301), NADH-ubiquinone oxidoreductase, putative (spot 6815) and 14-3-3-like protein (spot 3016) were up-regulated. The 14-3-3 proteins act as the regulators for many important biological processes, such as plant metabolism, development, transcription, stress response, and signal transduction (Pandey and Mann 2000;Radwan et al 2012). The study of Pinus contorta roots found that the 14-3-3-like protein was expressed 0-3 days after cutting, but in this study the 14-3-3-like protein was expressed in EAR; this may be because the 14-3-3-like protein performs different functions in different cells.…”

Section: Functional Analysis Of the Identified Proteinsmentioning

confidence: 99%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

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Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

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14-3-3 Proteins SGF14c and SGF14l Play Critical Roles during Soybean Nodulation

Cited by 32 publications

References 52 publications

Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis

Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

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